LOCUS       sequence-352992-       10099 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10099)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10099)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0213
FEATURES             Location/Qualifiers
     source          1..10099
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     RBS             1694..1702
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     misc_feature    3037..3625
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3090..3110)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3508..3519)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3697..3930
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4008..4129
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4045..4064)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4099..4118
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4169..4304
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4324..4343)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4325..4343)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4353..4370)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4353..4369)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4362..4384)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4511..4966
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4598..4617)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4808..4829
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4992..5096
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5097..5165,5166..5957)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5315..5334)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6128..6716
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6617..6636
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6870..6887
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7004..7025
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7040..7045,7046..7063,7064..7070)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7078..7094
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7083..7105
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7102..7118
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7102..7118
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7137..7157
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7139..7157
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7185..7411
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7412..7592
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7639..7764
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8257..8490
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8675..8719
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8950..9160
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8972..9168
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9019..9154
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9200..9802
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9200..9219)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9696..9716
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9845..9962
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgat
     1201 gacacctcaa gttatcactc cccagcaaat gcagcagatc ctccagcaac aagtgctgag
     1261 ccctcagcag ctccaggttc tcctccagca gcagcaggcc ctcatgcttc aacagcagca
     1321 gcttcaagag ttttataaaa aacaacagga acagttgcag cttcaacttt tacaacaaca
     1381 acatgctgga aaacagccta aagagcaaca gcaggtggct acccagcagt tggcttttca
     1441 gcagcagctt ttacagatgc agcagttaca gcagcagcac ctcctgtctt tgcagcgcca
     1501 aggccttctg acaattcagc ccgggcagcc tgcccttccc cttcaacctc ttgctcaagg
     1561 catgattcca acagaactgc agcagctctg gaaagaagtg acaagtgctc atactgcaga
     1621 agaaaccaca ggcaacaatc acagcagttt ggatctgacc acgacatgtg tctcctcctc
     1681 tgcaccttcc aagacctcct taataatgaa cccacatgcc tctaccaatg gacagctctc
     1741 agtccacact cccaaaaggg aaagtttgtc ccatgaggag cacccccata gccatcctct
     1801 ctatggacat ggtgtatgca agtggccagg ctgtgaagca gtgtgcgaag atttccaatc
     1861 atttctaaaa catctcaaca gtgagcatgc gctggacgat agaagtacag cccaatgtag
     1921 agtacaaatg caggttgtac agcagttaga gctacagctt gcaaaagaca aagaacgcct
     1981 gcaagccatg atgacccacc tgcatgtgaa gtctacagaa cccaaagccg cccctcagcc
     2041 cttgaatctg gtatcaagtg tcactctctc caagtccgca tcggaggctt ctccacagag
     2101 cttacctcat actccaacga ccccaaccgc ccccctgact cccgtcaccc aaggcccctc
     2161 tgtcatcaca accaccagca tgcacacggt gggacccatc cgcaggcggt actcagacaa
     2221 atacaacgtg cccatttcgt cagcagatat tgcgcagaac caagaatttt ataagaacgc
     2281 agaagttaga ccaccattta catatgcatc tttaattagg caggccattc tcgaatctcc
     2341 agaaaagcag ctaacactaa atgagatcta taactggttc acacgaatgt ttgcttactt
     2401 ccgacgcaac gcggccacgt ggaagaatgc agtgcgtcat aatcttagtc ttcacaagtg
     2461 ttttgtgcga gtagaaaacg ttaaaggggc agtatggaca gtggatgaag tagaattcca
     2521 aaaacgaagg ccacaaaaga tcagtggtaa cccttccctt attaaaaaca tgcagagcag
     2581 ccacgcctac tgcacacctc tcaatgcagc tttacaggct tcaatggctg agaatagtat
     2641 acctctatac actaccgctt ccatgggaaa tcccactctg ggcaacttag ccagcgcaat
     2701 acgggaagag ctgaacgggg caatggagca taccaacagc aacgagagtg acagcagtcc
     2761 aggcagatct cctatgcaag ccgtgcatcc tgtacacgtc aaagaagagc ccctcgatcc
     2821 agaggaagct gaagggcccc tgtccttagt gacaacagcc aaccacagtc cagattttga
     2881 ccatgacaga gattacgaag atgaaccagt aaacgaggac atggagtagt aatgaactag
     2941 tccgtaactt gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgattg
     3001 gtcctatctt atgacgttac gacgcgttaa gtcgacaatc aacctctgga ttacaaaatt
     3061 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     3121 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     3181 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     3241 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     3301 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     3361 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     3421 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
     3481 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     3541 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     3601 atctcccttt gggccgcctc cccgcgtcga ctttaagacc aatgacttac aaggcagctg
     3661 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     3721 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     3781 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     3841 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     3901 ccttttagtc agtgtggaaa atctctagca gtacgtatag tagttcatgt catcttatta
     3961 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg
     4021 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     4081 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc
     4141 tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     4201 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     4261 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat
     4321 tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac
     4381 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     4441 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     4501 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     4561 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     4621 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     4681 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     4741 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     4801 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     4861 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     4921 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc
     4981 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     5041 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     5101 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     5161 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     5221 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     5281 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     5341 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     5401 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     5461 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     5521 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     5581 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     5641 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     5701 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     5761 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     5821 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     5881 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     5941 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     6001 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     6061 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     6121 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     6181 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     6241 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     6301 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     6361 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     6421 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     6481 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     6541 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     6601 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     6661 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     6721 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     6781 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     6841 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     6901 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     6961 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     7021 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     7081 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa
     7141 ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt cttatgcaat
     7201 actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac aaggagagaa
     7261 aaagcaccgt gcatgccgat tggtggaagt aaggtggtac gatcgtgcct tattaggaag
     7321 gcaacagacg ggtctgacat ggattggacg aaccactgaa ttgccgcatt gcagagatat
     7381 tgtatttaag tgcctagctc gatacataaa cgggtctctc tggttagacc agatctgagc
     7441 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     7501 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     7561 acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg
     7621 aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca
     7681 agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag
     7741 gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa
     7801 aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca
     7861 agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt
     7921 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
     7981 ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc
     8041 aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa
     8101 gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt
     8161 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag
     8221 aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt
     8281 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca
     8341 attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca
     8401 gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt
     8461 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat
     8521 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg
     8581 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
     8641 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
     8701 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
     8761 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
     8821 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
     8881 cccgagggga cccatgcatt gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     8941 ggctccccag caggcagaag tatgcaaagc atgcgtctca attagtcagc aaccatagtc
     9001 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     9061 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     9121 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctttctaga
     9181 ggtaccaccg gagcttacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga
     9241 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
     9301 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
     9361 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgccgtggc
     9421 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
     9481 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
     9541 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
     9601 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
     9661 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
     9721 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
     9781 ccgcaagccc ggtgccagtt aacgaattct agatcttgag acaaatggca gtattcatcc
     9841 acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
     9901 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt
     9961 ttcgggttta ttacagggac agcagagatc cactttggcg ccggctcgag gcctgcaggt
    10021 gcaaagatgg ataaagtttt aaacagagag gaatctttgc agctaatgga ccttctaggt
    10081 cttgaaagga gtgggaatt
//
