LOCUS       sequence-352993-       10660 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10660)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10660)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1510
FEATURES             Location/Qualifiers
     source          1..10660
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1190..1199
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    3598..4186
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3651..3671)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4069..4080)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4258..4491
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4569..4690
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4606..4625)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4660..4679
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4730..4865
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4885..4904)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4886..4904)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4914..4931)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4914..4930)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4923..4945)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5072..5527
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5159..5178)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5369..5390
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5553..5657
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5658..5726,5727..6518)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5876..5895)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6689..7277
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7178..7197
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7431..7448
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7565..7586
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7601..7606,7607..7624,7625..7631)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7639..7655
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7644..7666
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7663..7679
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7663..7679
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7698..7718
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7700..7718
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7746..7972
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7973..8153
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8200..8325
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8818..9051
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9236..9280
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9511..9721
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9533..9729
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9580..9715
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9761..10363
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9761..9780)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10257..10277
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10406..10523
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatggc
     1201 gggctggatc caggcccagc agctgcaggg agacgcgctg cgccagatgc aggtgctgta
     1261 cggccagcac ttccccatcg aggtccggca ctacttggcc cagtggattg agagccagcc
     1321 atgggatgcc attgacttgg acaatcccca ggacagagcc caagccaccc agctcctgga
     1381 gggcctggtg caggagctgc agaagaaggc ggagcaccag gtgggggaag atgggttttt
     1441 actgaagatc aagctggggc actacgccac gcagctccag tgcagctctc cggctgggat
     1501 cctggttgac gccatgtccc agaagcacct tcagatcaac cagacatttg aggagctgcg
     1561 actggtcacg caggacacag agaatgagct gaagaaactg cagcagactc aggagtactt
     1621 catcatccag taccaggaga gcctgaggat ccaagctcag tttgcccagc tggcccagct
     1681 gagcccccag gagcgtctga gccgggagac ggccctccag cagaagcagg tgtctctgga
     1741 ggcctggttg cagcgtgagg cacagacact gcagcagtac cgcgtggagc tggccgagaa
     1801 gcaccagaag accctgcagc tgctgcggaa gcagcagacc atcatcctgg atgacgagct
     1861 gatccagtgg aagcggcggc agcagctggc cgggaacggc gggccccccg agggcagcct
     1921 ggacgtgcta cagtcctggt gtgagaagtt ggccgagatc atctggcaga accggcagca
     1981 gatccgcagg gctgagcacc tctgccagca gctgcccatc cccggcccag tggaggagat
     2041 gctggccgag gtcaacgcca ccatcacgga cattatctca gccctggtga ccagcacatt
     2101 catcattgag aagcagcctc ctcaggtcct gaagacccag accaagtttg cagccaccgt
     2161 acgcctgctg gtgggcggga agctgaacgt gcacatgaat cccccccagg tgaaggccac
     2221 catcatcagt gagcagcagg ccaagtctct gcttaaaaat gagaacaccc gcaacgagtg
     2281 cagtggtgag atcctgaaca actgctgcgt gatggagtac caccaagcca cgggcaccct
     2341 cagtgcccac ttcaggaaca tgtcactgaa gaggatcaag cgtgctgacc ggcggggtgc
     2401 agagtccgtg acagaggaga agttcacagt cctgtttgag tctcagttca gtgttggcag
     2461 caatgagctt gtgttccagg tgaagactct gtccctacct gtggttgtca tcgtccacgg
     2521 cagccaggac cacaatgcca cggctactgt gctgtgggac aatgcctttg ctgagccggg
     2581 cagggtgcca tttgccgtgc ctgacaaagt gctgtggccg cagctgtgtg aggcgctcaa
     2641 catgaaattc aaggccgaag tgcagagcaa ccggggcctg accaaggaga acctcgtgtt
     2701 cctggcgcag aaactgttca acaacagcag cagccacctg gaggactaca gtggcctgtc
     2761 cgtgtcctgg tcccagttca acagggagaa cttgccgggc tggaactaca ccttctggca
     2821 gtggtttgac ggggtgatgg aggtgttgaa gaagcaccac aagccccact ggaatgatgg
     2881 ggccatccta ggttttgtga ataagcaaca ggcccacgac ctgctcatca acaagcccga
     2941 cgggaccttc ttgttgcgct ttagtgactc agaaatcggg ggcatcacca tcgcctggaa
     3001 gtttgactcc ccggaacgca acctgtggaa cctgaaacca ttcaccacgc gggatttctc
     3061 catcaggtcc ctggctgacc ggctggggga cctgagctat ctcatctatg tgtttcctga
     3121 ccgccccaag gatgaggtct tctccaagta ctacactcct gtgctggcta aagctgttga
     3181 tggatatgtg aaaccacaga tcaagcaagt ggtccctgag tttgtgaatg catctgcaga
     3241 tgctgggggc agcagcgcca cgtacatgga ccaggccccc tccccagctg tgtgccccca
     3301 ggctccctat aacatgtacc cacagaaccc tgaccatgta ctcgatcagg atggagaatt
     3361 cgacctggat gagaccatgg atgtggccag gcacgtggag gaactcttac gccgaccaat
     3421 ggacagtctt gactcccgcc tctcgccccc tgccggtctt ttcacctctg ccagaggctc
     3481 cctctcatag taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat
     3541 atcttgtgga aaggacgaga gggacaccct agtagagaaa cgacgcgtta agtcgacaat
     3601 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     3661 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     3721 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     3781 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     3841 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     3901 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     3961 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     4021 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     4081 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     4141 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcgtcg actttaagac
     4201 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     4261 aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct
     4321 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     4381 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     4441 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtacgtata
     4501 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
     4561 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     4621 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     4681 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct
     4741 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     4801 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     4861 aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc
     4921 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     4981 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     5041 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
     5101 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     5161 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     5221 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     5281 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     5341 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     5401 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     5461 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
     5521 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     5581 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     5641 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     5701 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     5761 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     5821 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     5881 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     5941 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     6001 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     6061 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     6121 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     6181 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     6241 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     6301 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     6361 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     6421 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     6481 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     6541 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     6601 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     6661 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     6721 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     6781 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     6841 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     6901 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     6961 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     7021 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     7081 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     7141 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     7201 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     7261 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     7321 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     7381 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     7441 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     7501 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     7561 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     7621 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     7681 atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag
     7741 cttaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
     7801 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
     7861 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
     7921 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
     7981 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     8041 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     8101 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
     8161 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
     8221 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
     8281 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
     8341 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
     8401 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
     8461 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
     8521 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
     8581 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
     8641 agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag
     8701 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     8761 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     8821 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     8881 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     8941 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     9001 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     9061 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     9121 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     9181 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     9241 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     9301 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     9361 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     9421 gtttcagacc cacctcccaa ccccgagggg acccatgcat tgcatctcaa ttagtcagca
     9481 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcgtctc
     9541 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     9601 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     9661 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     9721 ttttgcaaaa agctttctag aggtaccacc ggagcttacc atgaccgagt acaagcccac
     9781 ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt
     9841 cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac
     9901 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc
     9961 ggacgacggc gccgccgtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt
    10021 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca
    10081 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac
    10141 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg
    10201 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa
    10261 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg
    10321 accgcgcacc tggtgcatga cccgcaagcc cggtgccagt taacgaattc tagatcttga
    10381 gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg
    10441 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
    10501 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat ccactttggc
    10561 gccggctcga ggcctgcagg tgcaaagatg gataaagttt taaacagaga ggaatctttg
    10621 cagctaatgg accttctagg tcttgaaagg agtgggaatt
//
