LOCUS       sequence-352995-        8781 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables spatial detection of 
            CRISPR screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8781)
  AUTHORS   Dhainaut M, Rose SA, Akturk G, Wroblewska A, Nielsen SR, Park ES, 
            Buckup M, Roudko V, Pia L, Sweeney R, Le Berichel J, Wilk CM, 
            Bektesevic A, Lee BH, Bhardwaj N, Rahman AH, Baccarini A, Gnjatic S,
            Pe'er D, Merad M, Brown BD
  TITLE     Spatial CRISPR genomics identifies regulators of the tumor 
            microenvironment.
  JOURNAL   Cell. 2022 Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015.
  PUBMED    35290801
REFERENCE   2  (bases 1 to 8781)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2022 
            Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015."
COMMENT     Sequence Label: pLKO_C.NWS.VSVg_mCherry-NLS
FEATURES             Location/Qualifiers
     source          1..8781
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     147..166
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    511..1099
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(564..584)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(982..993)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             1174..1407
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1479..1600
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1516..1535)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1570..1589
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1640..1775
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1795..1814)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1796..1814)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1824..1841)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1824..1840)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1833..1855)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1982..2437
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2069..2088)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2279..2300
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2463..2567
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2568..2636,2637..3428)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2786..2805)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3599..4187
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4088..4107
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4341..4358
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4475..4496
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4511..4516,4517..4534,4535..4541)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4549..4565
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4554..4576
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4573..4589
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4573..4589
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4608..4628
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4610..4628
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4656..4882
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4883..5063
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5110..5235
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5728..5961
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6146..6190
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        6488..6736
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     6488..6508
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     6659..6678
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        6755..6830
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    6878..6995
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7135..8313
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          7366..8304
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     8261..8281
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      8362..8371
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8374..8409
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             8431..8454
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             8473..8505
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     CDS             join(8515..8781,1..441)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(8660..8678)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga cggcggcgtg
       61 gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa ggtgaagctg
      121 cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat gggctgggag
      181 gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat caagcagagg
      241 ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta caaggccaag
      301 aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat cacctcccac
      361 aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca ctccaccggc
      421 ggcatggacg agctgtacaa gaaacggcca gccgaggata tggaggaaga acaagccttt
      481 aaacgatctc gataaagcgg ccgcgtcgac aatcaacctc tggattacaa aatttgtgaa
      541 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
      601 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
      661 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
      721 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
      781 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
      841 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
      901 gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat tctgcgcggg
      961 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     1021 ctgccggctc tgcggcctct tccgcatctt cgccttcgcc ctcagacgag tcggatctcc
     1081 ctttgggccg cctccccgcc tgggtacctt taagaccaat gacttacaag gcagctgtag
     1141 atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa
     1201 gataagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
     1261 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     1321 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     1381 tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt
     1441 tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata
     1501 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
     1561 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat
     1621 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
     1681 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
     1741 attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat
     1801 agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     1861 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     1921 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     1981 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
     2041 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
     2101 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
     2161 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     2221 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     2281 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     2341 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     2401 aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg
     2461 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     2521 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     2581 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     2641 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     2701 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     2761 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     2821 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     2881 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     2941 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     3001 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     3061 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     3121 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     3181 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     3241 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     3301 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     3361 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     3421 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     3481 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     3541 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     3601 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     3661 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     3721 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     3781 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     3841 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     3901 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     3961 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     4021 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     4081 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     4141 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     4201 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     4261 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     4321 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     4381 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     4441 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     4501 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     4561 aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc
     4621 actaaaggga acaaaagctg gagctgcaag cttaatgtag tcttatgcaa tactcttgta
     4681 gtcttgcaac atggtaacga tgagttagca acatgcctta caaggagaga aaaagcaccg
     4741 tgcatggcga ttggtggaag taaggtggta cgatcgtgcc ttattaggaa ggcaacagac
     4801 gggtctgaca tggattggac gaaccactga attgccgcat tgcagagata ttgtatttaa
     4861 gtgcctagct cgatacataa acgggtctct ctggttagac cagatctgag cctgggagct
     4921 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     4981 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     5041 gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa
     5101 ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag
     5161 gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat
     5221 gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg
     5281 ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag
     5341 ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata
     5401 ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat
     5461 acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct
     5521 ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct
     5581 gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata
     5641 taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt
     5701 gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc
     5761 aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc
     5821 tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt
     5881 gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata
     5941 cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac
     6001 tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac
     6061 gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat
     6121 tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg
     6181 ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa aattattcat
     6241 aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa
     6301 tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg
     6361 acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat
     6421 tcgattagtg aacggatctc gacggtatcg atcacgagac tagtatcgag cggccgcccc
     6481 cttcaccgag ggcctatttc ccatgattcc ttcatatttg catatacgat acaaggctgt
     6541 tagagagata attggaatta atttgactgt aaacacaaag atattagtac aaaatacgtg
     6601 acgtagaaag taataatttc ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga
     6661 ctatcatatg cttaccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg
     6721 gaaaggacga aacaccgggt cttcgagaag acctgtttta gagctagaaa tagcaagtta
     6781 aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc tttttttaat
     6841 tctcgacctc gagacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
     6901 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
     6961 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
     7021 atccactttg gccgcggctc gaggcgataa gctttgcaaa gatggataaa gttttaaaca
     7081 gagaggaatc tttgcagcta atggaccttc taggtcttga aaggagtggg aattggctcc
     7141 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg
     7201 gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc
     7261 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc
     7321 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggtaag tgccgtgtgt
     7381 ggttcccgcg ggcctggcct ctttacgggt tatggccctt gcgtgccttg aattacttcc
     7441 acctggctgc agtacgtgat tcttgatccc gagcttcggg ttggaagtgg gtgggagagt
     7501 tcgaggcctt gcgcttaagg agccccttcg cctcgtgctt gagttgaggc ctggcctggg
     7561 cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc tgctttcgat
     7621 aagtctctag ccatttaaaa tttttgatga cctgctgcga cgcttttttt ctggcaagat
     7681 agtcttgtaa atgcgggcca agatctgcac actggtattt cggtttttgg ggccgcgggc
     7741 ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg
     7801 ccaccgagaa tcggacgggg gtagtctcaa gctggccggc ctgctctggt gcctggcctc
     7861 gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc accagttgcg
     7921 tgagcggaaa gatggccgct tcccggccct gctgcaggga gctcaaaatg gaggacgcgg
     7981 cgctcgggag agcgggcggg tgagtcaccc acacaaagga aaagggcctt tccgtcctca
     8041 gccgtcgctt catgtgactc cacggagtac cgggcgccgt ccaggcacct cgattagttc
     8101 tcgagctttt ggagtacgtc gtctttaggt tggggggagg ggttttatgc gatggagttt
     8161 ccccacactg agtgggtgga gactgaagtt aggccagctt ggcacttgat gtaattctcc
     8221 ttggaatttg ccctttttga gtttggatct tggttcattc tcaagcctca gacagtggtt
     8281 caaagttttt ttcttccatt tcaggtgtcg tgaggaattc tgcagtcgat cgacggtacc
     8341 gcgggcccgg gatccacggt cgccaccatg ggcgaggacc aggtggaccc caggctgatc
     8401 gacggcaagg gaggaggcgg gggagggaac tggtcccacc cccaattcga gaaaggagga
     8461 ggcgggggag ggtatacaga tatcgaaatg aatagactcg gaaagggcat gctgatggtg
     8521 agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac
     8581 atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc
     8641 tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc
     8701 tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc
     8761 gacatccccg actacttgaa g
//
