LOCUS       sequence-352998-        8811 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables spatial detection of 
            CRISPR screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8811)
  AUTHORS   Dhainaut M, Rose SA, Akturk G, Wroblewska A, Nielsen SR, Park ES, 
            Buckup M, Roudko V, Pia L, Sweeney R, Le Berichel J, Wilk CM, 
            Bektesevic A, Lee BH, Bhardwaj N, Rahman AH, Baccarini A, Gnjatic S,
            Pe'er D, Merad M, Brown BD
  TITLE     Spatial CRISPR genomics identifies regulators of the tumor 
            microenvironment.
  JOURNAL   Cell. 2022 Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015.
  PUBMED    35290801
REFERENCE   2  (bases 1 to 8811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2022 
            Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015."
COMMENT     Sequence Label: pLKO_C.Ollas.S_mCherry-NLS
FEATURES             Location/Qualifiers
     source          1..8811
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      6..141
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(161..180)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(162..180)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(190..207)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(190..206)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(199..221)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      348..803
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(435..454)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     645..666
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        829..933
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(934..1002,1003..1794)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1152..1171)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1965..2553
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2454..2473
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2707..2724
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2841..2862
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2877..2882,2883..2900,2901..2907)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2915..2931
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2920..2942
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2939..2955
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2939..2955
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2974..2994
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2976..2994
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        3022..3248
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             3249..3429
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3476..3601
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4094..4327
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4512..4556
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        4854..5102
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     4854..4874
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     5025..5044
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        5121..5196
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    5244..5361
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5501..6679
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5732..6670
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     6627..6647
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      6728..6737
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6740..6775
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             6794..6835
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             6857..6901
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             6911..7618
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(7056..7074)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7324..7343
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    7688..8276
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7741..7761)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8159..8170)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             8351..8584
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8656..8777
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8693..8712)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8747..8766
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
ORIGIN
        1 cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa
       61 ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt
      121 gaggaggctt ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta
      181 cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
      241 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
      301 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag
      361 cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag
      421 cgccctagcg cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt
      481 tccccgtcaa gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca
      541 cctcgacccc aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata
      601 gacggttttt cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca
      661 aactggaaca acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc
      721 gatttcggcc tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa
      781 caaaatatta acgcttacaa tttaggtggc acttttcggg gaaatgtgcg cggaacccct
      841 atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
      901 taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
      961 cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     1021 aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     1081 aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     1141 tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca agagcaactc
     1201 ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     1261 catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     1321 aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     1381 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     1441 gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac aacgttgcgc
     1501 aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     1561 gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     1621 gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     1681 gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     1741 gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     1801 gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     1861 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     1921 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     1981 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     2041 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     2101 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     2161 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     2221 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     2281 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     2341 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     2401 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     2461 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     2521 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     2581 ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     2641 gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     2701 gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc caatacgcaa accgcctctc
     2761 cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg
     2821 ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta
     2881 cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca
     2941 ggaaacagct atgaccatga ttacgccaag cgcgcaatta accctcacta aagggaacaa
     3001 aagctggagc tgcaagctta atgtagtctt atgcaatact cttgtagtct tgcaacatgg
     3061 taacgatgag ttagcaacat gccttacaag gagagaaaaa gcaccgtgca tggcgattgg
     3121 tggaagtaag gtggtacgat cgtgccttat taggaaggca acagacgggt ctgacatgga
     3181 ttggacgaac cactgaattg ccgcattgca gagatattgt atttaagtgc ctagctcgat
     3241 acataaacgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     3301 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     3361 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     3421 tctctagcag tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc
     3481 tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt
     3541 gagtacgcca aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt
     3601 cagtattaag cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg
     3661 aaagaaaaaa tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc
     3721 agttaatcct ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca
     3781 accatccctt cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct
     3841 ctattgtgtg catcaaagga tagagataaa agacaccaag gaagctttag acaagataga
     3901 ggaagagcaa aacaaaagta agaccaccgc acagcaagcg gccgctgatc ttcagacctg
     3961 gaggaggaga tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa
     4021 ttgaaccatt aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa
     4081 gagcagtggg aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg
     4141 gcgcagcgtc aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc
     4201 agcagaacaa tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct
     4261 ggggcatcaa gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac
     4321 agctcctggg gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga
     4381 atgctagttg gagtaataaa tctctggaac agatttggaa tcacacgacc tggatggagt
     4441 gggacagaga aattaacaat tacacaagct taatacactc cttaattgaa gaatcgcaaa
     4501 accagcaaga aaagaatgaa caagaattat tggaattaga taaatgggca agtttgtgga
     4561 attggtttaa cataacaaat tggctgtggt atataaaatt attcataatg atagtaggag
     4621 gcttggtagg tttaagaata gtttttgctg tactttctat agtgaataga gttaggcagg
     4681 gatattcacc attatcgttt cagacccacc tcccaacccc gaggggaccc gacaggcccg
     4741 aaggaataga agaagaaggt ggagagagag acagagacag atccattcga ttagtgaacg
     4801 gatctcgacg gtatcgatca cgagactagt atcgagcggc cgcccccttc accgagggcc
     4861 tatttcccat gattccttca tatttgcata tacgatacaa ggctgttaga gagataattg
     4921 gaattaattt gactgtaaac acaaagatat tagtacaaaa tacgtgacgt agaaagtaat
     4981 aatttcttgg gtagtttgca gttttaaaat tatgttttaa aatggactat catatgctta
     5041 ccgtaacttg aaagtatttc gatttcttgg ctttatatat cttgtggaaa ggacgaaaca
     5101 ccgggtcttc gagaagacct gttttagagc tagaaatagc aagttaaaat aaggctagtc
     5161 cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt tttaattctc gacctcgaga
     5221 caaatggcag tattcatcca caattttaaa agaaaagggg ggattggggg gtacagtgca
     5281 ggggaaagaa tagtagacat aatagcaaca gacatacaaa ctaaagaatt acaaaaacaa
     5341 attacaaaaa ttcaaaattt tcgggtttat tacagggaca gcagagatcc actttggccg
     5401 cggctcgagg cgataagctt tgcaaagatg gataaagttt taaacagaga ggaatctttg
     5461 cagctaatgg accttctagg tcttgaaagg agtgggaatt ggctccggtg cccgtcagtg
     5521 ggcagagcgc acatcgccca cagtccccga gaagttgggg ggaggggtcg gcaattgaac
     5581 cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt gatgtcgtgt actggctccg
     5641 cctttttccc gagggtgggg gagaaccgta tataagtgca gtagtcgccg tgaacgttct
     5701 ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc gtgtgtggtt cccgcgggcc
     5761 tggcctcttt acgggttatg gcccttgcgt gccttgaatt acttccacct ggctgcagta
     5821 cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg gagagttcga ggccttgcgc
     5881 ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg cctgggcgct ggggccgccg
     5941 cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct ttcgataagt ctctagccat
     6001 ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg caagatagtc ttgtaaatgc
     6061 gggccaagat ctgcacactg gtatttcggt ttttggggcc gcgggcggcg acggggcccg
     6121 tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga gcgcggccac cgagaatcgg
     6181 acgggggtag tctcaagctg gccggcctgc tctggtgcct ggcctcgcgc cgccgtgtat
     6241 cgccccgccc tgggcggcaa ggctggcccg gtcggcacca gttgcgtgag cggaaagatg
     6301 gccgcttccc ggccctgctg cagggagctc aaaatggagg acgcggcgct cgggagagcg
     6361 ggcgggtgag tcacccacac aaaggaaaag ggcctttccg tcctcagccg tcgcttcatg
     6421 tgactccacg gagtaccggg cgccgtccag gcacctcgat tagttctcga gcttttggag
     6481 tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg gagtttcccc acactgagtg
     6541 ggtggagact gaagttaggc cagcttggca cttgatgtaa ttctccttgg aatttgccct
     6601 ttttgagttt ggatcttggt tcattctcaa gcctcagaca gtggttcaaa gtttttttct
     6661 tccatttcag gtgtcgtgag gaattctgca gtcgatcgac ggtaccgcgg gcccgggatc
     6721 cacggtcgcc accatgggcg aggaccaggt ggaccccagg ctgatcgacg gcaagggagg
     6781 aggcggggga gggagcggct tcgccaacga gctgggcccc aggctgatgg gcaagtgcgg
     6841 aggaggcggg ggagggaagg agaccgccgc cgccaagttc gagaggcagc acatggacag
     6901 cggcatgctg atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat
     6961 gcgcttcaag gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga
     7021 gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg
     7081 ccccctgccc ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta
     7141 cgtgaagcac cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa
     7201 gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc
     7261 cctgcaggac ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga
     7321 cggccccgta atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc
     7381 cgaggacggc gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca
     7441 ctacgacgct gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc
     7501 ctacaacgtc aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga
     7561 acagtacgaa cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaagaa
     7621 acggccagcc gaggatatgg aggaagaaca agcctttaaa cgatctcgat aaagcggccg
     7681 cgtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     7741 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     7801 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     7861 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     7921 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     7981 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     8041 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg
     8101 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     8161 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     8221 gcatcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg
     8281 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     8341 aaggggggac tggaagggct aattcactcc caacgaagat aagatctgct ttttgcttgt
     8401 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     8461 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     8521 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     8581 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
     8641 tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     8701 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     8761 ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact c
//
