LOCUS       sequence-35300-e         814 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 814)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 814)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: HL 3186 (=HL 716 + pHL 282)
FEATURES             Location/Qualifiers
     source          1..814
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    146..164
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    171..189
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      complement(256..283)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(375..461)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     614..631
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     primer_bind     complement(717..737)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 ggcctcgccc tcgccctcga tctcgaactc gtggccgttc acggagccct ccatgtgcac
       61 cttgaagcgc atgaactcct tgatgatggc catgttatcc tcctcgccct tgctcaccat
      121 tttttttcct ccttacccgg gagtatctct atcactgata gggatgtcaa tctctatcac
      181 tgatagggag gatcctgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaaaga
      241 gtttgtagaa acgcaaaaag gccatccgtc aggatggcct tctgcttaat ttgatgcctg
      301 gcagtttatg gcgggcgtcc tgcccgccac cctccgggcc gttgcttcgc aacgttcaaa
      361 tccgctcccg gcggatttgt cctactcagg agagcgttca ccgacaaaca acagataaaa
      421 cgaaaggccc agtctttcga ctgagccttt cgttttattt gatgcctggc agttccctac
      481 tctcgcatgg ggagacccca cactaccatc ggcgctacgg cgtttcactt ctgagttcgg
      541 catggggtca ggtgggacca ccgcgctact gccgccaggc aaattctgtt ttatcagacc
      601 gcttctgcgt tctgatttaa tctgtatcag gctgaaaatc ttctctcatc cgccaaaaca
      661 gccaagctag cttttatttg tagagctcat ccatgccatg tgtaatccca gcagctgtta
      721 caaactcaag aaggaccatg tggtctctct tttcgttggg atctttcgaa agggcagatt
      781 gtgtggacag gtaatggttg tctggtaaaa ggac
//
