LOCUS       sequence-353002-       10294 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10294)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10294)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0258
FEATURES             Location/Qualifiers
     source          1..10294
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    3232..3820
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3285..3305)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3703..3714)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3892..4125
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4203..4324
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4240..4259)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4294..4313
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4364..4499
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4519..4538)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4520..4538)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4548..4565)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4548..4564)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4557..4579)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4706..5161
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4793..4812)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5003..5024
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5187..5291
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5292..5360,5361..6152)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5510..5529)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6323..6911
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6812..6831
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7065..7082
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7199..7220
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7235..7240,7241..7258,7259..7265)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7273..7289
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7278..7300
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7297..7313
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7297..7313
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7332..7352
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7334..7352
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7380..7606
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7607..7787
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7834..7959
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8452..8685
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8870..8914
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9145..9355
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9167..9363
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9214..9349
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9395..9997
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9395..9414)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9891..9911
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10040..10157
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgtt
     1201 ttcacctcct gtgagcagtg ggaaaaatgg accaacttct ttggcaagtg gacattttac
     1261 tggctcaaat gtagaagaca gaagtagctc agggtcctgg gggaatggag gacatccaag
     1321 cccgtccagg aactatggag atgggactcc ctatgaccac atgaccagca gggaccttgg
     1381 gtcacatgac aatctctctc caccttttgt caattccaga atacaaagta aaacagaaag
     1441 gggctcatac tcatcttatg ggagagaatc aaacttacag ggttgccacc agagtctcct
     1501 tggaggtgac atggatatgg gcaacccagg aaccctttcg cccaccaaac ctggttccca
     1561 gtactatcag tattctagca ataatccccg aaggaggcct cttcacagta gtgccatgga
     1621 ggtacagaca aagaaagttc gaaaagttcc tccaggtttg ccatcttcag tctatgctcc
     1681 atcagcaagc actgccgact acaataggga ctcgccaggc tatccttcct ccaaaccagc
     1741 aaccagcact ttccctagct ccttcttcat gcaagatggc catcacagca gtgacccttg
     1801 gagctcctcc agtgggatga atcagcctgg ctatgcagga atgttgggca actcttctca
     1861 tattccacag tccagcagct actgtagcct gcatccacat gaacgtttga gctatccatc
     1921 acactcctca gcagacatca attccagtct tcctccgatg tccactttcc atcgtagtgg
     1981 tacaaaccat tacagcacct cttcctgtac gcctcctgcc aacgggacag acagtataat
     2041 ggcaaataga ggaagcgggg cagccggcag ctcccagact ggagatgctc tggggaaagc
     2101 acttgcttcg atctattctc cagatcacac taacaacagc ttttcatcaa acccttcaac
     2161 tcctgttggc tctcctccat ctctctcagc aggcacagct gtttggtcta gaaatggagg
     2221 acaggcctca tcgtctccta attatgaagg acccttacac tctttgcaaa gccgaattga
     2281 agatcgttta gaaagactgg atgatgctat tcatgttctc cggaaccatg cagtgggccc
     2341 atccacagct atgcctggtg gtcatgggga catgcatgga atcattggac cttctcataa
     2401 tggagccatg ggtggtctgg gctcagggta tggaaccggc cttctttcag ccaacagaca
     2461 ttcactcatg gtggggaccc atcgtgaaga tggcgtggcc ctgagaggca gccattctct
     2521 tctgccaaac caggttccgg ttccacagct tcctgtccag tctgcgactt cccctgacct
     2581 gaacccaccc caggaccctt acagaggcat gccaccagga ctacaggggc agagtgtctc
     2641 ctctggcagc tctgagatca aatccgatga cgagggtgat gagaacctgc aagacacgaa
     2701 atcttcggag gacaagaaat tagatgacga caagaaggat atcaaatcaa ttactagcaa
     2761 taatgacgat gaggacctga caccagagca gaaggcagag cgtgagaagg agcggaggat
     2821 ggccaacaat gcccgagagc gtctgcgggt ccgtgacatc aacgaggctt tcaaagagct
     2881 cggccgcatg gtgcagctcc acctcaagag tgacaagccc cagaccaagc tcctgatcct
     2941 ccaccaggcg gtggccgtca tcctcagtct ggagcagcaa gtccgagaaa ggaatctgaa
     3001 tccgaaagct gcgtgtctga aaagaaggga ggaagagaag gtgtcctcag agcctccccc
     3061 tctctccttg gccggcccac accctggaat gggagacgca tcgaatcaca tgggacagat
     3121 gtagtaatga actagtccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg
     3181 tggaaaggac gacgcctgct cgggcgtgta gtcgcaacgc gttaagtcga caatcaacct
     3241 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     3301 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     3361 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     3421 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     3481 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     3541 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     3601 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
     3661 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     3721 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
     3781 cctcagacga gtcggatctc cctttgggcc gcctccccgc gtcgacttta agaccaatga
     3841 cttacaaggc agctgtagat cttagccact ttttaaaaga aaagggggga ctggaagggc
     3901 taattcactc ccaacgaaga caagatctgc tttttgcttg tactgggtct ctctggttag
     3961 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     4021 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     4081 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtacg tatagtagtt
     4141 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
     4201 ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     4261 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     4321 cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc
     4381 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     4441 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     4501 agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt
     4561 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     4621 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     4681 ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt
     4741 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     4801 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     4861 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     4921 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     4981 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     5041 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     5101 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
     5161 taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac
     5221 attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa
     5281 aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat
     5341 tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc
     5401 agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga
     5461 gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg
     5521 cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc
     5581 agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag
     5641 taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc
     5701 tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg
     5761 taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg
     5821 acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac
     5881 ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac
     5941 cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg
     6001 agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg
     6061 tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg
     6121 agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac
     6181 tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg
     6241 ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg
     6301 tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc
     6361 aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc
     6421 tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt
     6481 agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc
     6541 taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact
     6601 caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac
     6661 agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag
     6721 aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg
     6781 gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg
     6841 tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga
     6901 gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt
     6961 ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct
     7021 ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg
     7081 aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
     7141 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
     7201 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
     7261 tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt
     7321 acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat
     7381 gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc
     7441 cttacaagga gagaaaaagc accgtgcatg ccgattggtg gaagtaaggt ggtacgatcg
     7501 tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc
     7561 gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt
     7621 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     7681 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     7741 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac
     7801 agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct
     7861 gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact
     7921 agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt
     7981 agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa
     8041 catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa
     8101 acatcagaag gctgtagaca aatactggga cagctacaac catcccttca gacaggatca
     8161 gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata
     8221 gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag
     8281 accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa
     8341 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
     8401 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
     8461 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
     8521 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
     8581 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
     8641 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
     8701 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
     8761 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
     8821 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
     8881 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
     8941 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
     9001 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
     9061 gacccacctc ccaaccccga ggggacccat gcattgcatc tcaattagtc agcaaccagg
     9121 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgcg tctcaattag
     9181 tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc gcccagttcc
     9241 gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc cgaggccgcc
     9301 tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct aggcttttgc
     9361 aaaaagcttt ctagaggtac caccggagct taccatgacc gagtacaagc ccacggtgcg
     9421 cctcgccacc cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga
     9481 ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct
     9541 gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga
     9601 cggcgccgcc gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc
     9661 cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat
     9721 ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg
     9781 cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga
     9841 ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc
     9901 cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg
     9961 cacctggtgc atgacccgca agcccggtgc cagttaacga attctagatc ttgagacaaa
    10021 tggcagtatt catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg
    10081 aaagaatagt agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta
    10141 caaaaattca aaattttcgg gtttattaca gggacagcag agatccactt tggcgccggc
    10201 tcgaggcctg caggtgcaaa gatggataaa gttttaaaca gagaggaatc tttgcagcta
    10261 atggaccttc taggtcttga aaggagtggg aatt
//
