LOCUS       sequence-353003-        8799 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables spatial detection of 
            CRISPR screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8799)
  AUTHORS   Dhainaut M, Rose SA, Akturk G, Wroblewska A, Nielsen SR, Park ES, 
            Buckup M, Roudko V, Pia L, Sweeney R, Le Berichel J, Wilk CM, 
            Bektesevic A, Lee BH, Bhardwaj N, Rahman AH, Baccarini A, Gnjatic S,
            Pe'er D, Merad M, Brown BD
  TITLE     Spatial CRISPR genomics identifies regulators of the tumor 
            microenvironment.
  JOURNAL   Cell. 2022 Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015.
  PUBMED    35290801
REFERENCE   2  (bases 1 to 8799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2022 
            Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015."
COMMENT     Sequence Label: pLKO_C.Ollas.VSVg_mCherry-NLS
FEATURES             Location/Qualifiers
     source          1..8799
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     134..153
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     387..404
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    521..542
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(557..562,563..580,581..587)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    595..611
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     600..622
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     619..635
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     619..635
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     654..674
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        656..674
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        702..928
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             929..1109
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1156..1281
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1774..2007
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2192..2236
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2534..2782
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2534..2554
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2705..2724
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        2801..2876
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    2924..3041
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3181..4359
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3412..4350
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4307..4327
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4408..4417
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4420..4455
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             4474..4515
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             4537..4569
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     CDS             4579..5286
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4724..4742)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4992..5011
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    5356..5944
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5409..5429)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5827..5838)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6019..6252
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6324..6445
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6361..6380)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6415..6434
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6485..6620
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6640..6659)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6641..6659)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6669..6686)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6669..6685)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6678..6700)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6827..7282
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6914..6933)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7124..7145
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7308..7412
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7413..7481,7482..8273)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7631..7650)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(8444..8799,1..233)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
       61 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
      121 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
      181 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
      241 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
      301 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
      361 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
      421 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
      481 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      541 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      601 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta
      661 accctcacta aagggaacaa aagctggagc tgcaagctta atgtagtctt atgcaatact
      721 cttgtagtct tgcaacatgg taacgatgag ttagcaacat gccttacaag gagagaaaaa
      781 gcaccgtgca tggcgattgg tggaagtaag gtggtacgat cgtgccttat taggaaggca
      841 acagacgggt ctgacatgga ttggacgaac cactgaattg ccgcattgca gagatattgt
      901 atttaagtgc ctagctcgat acataaacgg gtctctctgg ttagaccaga tctgagcctg
      961 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     1021 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     1081 cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa
     1141 gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga
     1201 ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag
     1261 agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa
     1321 attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc
     1381 agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga
     1441 caaatactgg gacagctaca accatccctt cagacaggat cagaagaact tagatcatta
     1501 tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag
     1561 gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg
     1621 gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata
     1681 taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag
     1741 agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg
     1801 agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt
     1861 attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca
     1921 tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga
     1981 aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg
     2041 caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa
     2101 tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct taatacactc
     2161 cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga
     2221 taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt
     2281 attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat
     2341 agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc
     2401 gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag
     2461 atccattcga ttagtgaacg gatctcgacg gtatcgatca cgagactagt atcgagcggc
     2521 cgcccccttc accgagggcc tatttcccat gattccttca tatttgcata tacgatacaa
     2581 ggctgttaga gagataattg gaattaattt gactgtaaac acaaagatat tagtacaaaa
     2641 tacgtgacgt agaaagtaat aatttcttgg gtagtttgca gttttaaaat tatgttttaa
     2701 aatggactat catatgctta ccgtaacttg aaagtatttc gatttcttgg ctttatatat
     2761 cttgtggaaa ggacgaaaca ccgggtcttc gagaagacct gttttagagc tagaaatagc
     2821 aagttaaaat aaggctagtc cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt
     2881 tttaattctc gacctcgaga caaatggcag tattcatcca caattttaaa agaaaagggg
     2941 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     3001 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
     3061 gcagagatcc actttggccg cggctcgagg cgataagctt tgcaaagatg gataaagttt
     3121 taaacagaga ggaatctttg cagctaatgg accttctagg tcttgaaagg agtgggaatt
     3181 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
     3241 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
     3301 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
     3361 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
     3421 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
     3481 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
     3541 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
     3601 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
     3661 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
     3721 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
     3781 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
     3841 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
     3901 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
     3961 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
     4021 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
     4081 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
     4141 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     4201 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     4261 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     4321 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gaattctgca gtcgatcgac
     4381 ggtaccgcgg gcccgggatc cacggtcgcc accatgggcg aggaccaggt ggaccccagg
     4441 ctgatcgacg gcaagggagg aggcggggga gggagcggct tcgccaacga gctgggcccc
     4501 aggctgatgg gcaagtgcgg aggaggcggg ggagggtata cagatatcga aatgaataga
     4561 ctcggaaagg gcatgctgat ggtgagcaag ggcgaggagg ataacatggc catcatcaag
     4621 gagttcatgc gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc
     4681 gagggcgagg gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc
     4741 aagggtggcc ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc
     4801 aaggcctacg tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag
     4861 ggcttcaagt gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag
     4921 gactcctccc tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc
     4981 ccctccgacg gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg
     5041 atgtaccccg aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac
     5101 ggcggccact acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg
     5161 cccggcgcct acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc
     5221 atcgtggaac agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg
     5281 tacaagaaac ggccagccga ggatatggag gaagaacaag cctttaaacg atctcgataa
     5341 agcggccgcg tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     5401 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     5461 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     5521 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     5581 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     5641 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     5701 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc
     5761 tttccatggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     5821 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     5881 cctcttccgc atcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     5941 ccgcctgggt acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt
     6001 taaaagaaaa ggggggactg gaagggctaa ttcactccca acgaagataa gatctgcttt
     6061 ttgcttgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     6121 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     6181 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     6241 aaatctctag cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga
     6301 aatgaatatc agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa
     6361 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     6421 tgtccaaact catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc
     6481 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
     6541 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
     6601 aggaggcttt tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac
     6661 gcgcgctcac tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa
     6721 cttaatcgcc ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc
     6781 accgatcgcc cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc
     6841 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
     6901 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
     6961 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
     7021 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
     7081 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
     7141 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
     7201 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattttaac
     7261 aaaatattaa cgcttacaat ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     7321 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     7381 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     7441 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     7501 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     7561 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     7621 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     7681 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     7741 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     7801 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     7861 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     7921 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     7981 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     8041 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     8101 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     8161 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     8221 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     8281 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     8341 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     8401 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     8461 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     8521 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     8581 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     8641 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     8701 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     8761 gaacgggggg ttcgtgcaca cagcccagct tggagcgaa
//
