LOCUS       sequence-353005-        8808 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables spatial detection of 
            CRISPR screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8808)
  AUTHORS   Dhainaut M, Rose SA, Akturk G, Wroblewska A, Nielsen SR, Park ES, 
            Buckup M, Roudko V, Pia L, Sweeney R, Le Berichel J, Wilk CM, 
            Bektesevic A, Lee BH, Bhardwaj N, Rahman AH, Baccarini A, Gnjatic S,
            Pe'er D, Merad M, Brown BD
  TITLE     Spatial CRISPR genomics identifies regulators of the tumor 
            microenvironment.
  JOURNAL   Cell. 2022 Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015.
  PUBMED    35290801
REFERENCE   2  (bases 1 to 8808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2022 
            Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015."
COMMENT     Sequence Label: pLKO_C.Ollas.V5_mCherry-NLS
FEATURES             Location/Qualifiers
     source          1..8808
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    228..349
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(265..284)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     319..338
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      389..524
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(544..563)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(545..563)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(573..590)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(573..589)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(582..604)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      731..1186
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(818..837)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1028..1049
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1212..1316
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1317..1385,1386..2177)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1535..1554)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2348..2936
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2837..2856
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3090..3107
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3224..3245
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3260..3265,3266..3283,3284..3290)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3298..3314
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3303..3325
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3322..3338
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3322..3338
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3357..3377
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3359..3377
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        3405..3631
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             3632..3812
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3859..3984
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4477..4710
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4895..4939
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        5237..5485
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     5237..5257
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     5408..5427
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        5504..5579
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    5627..5744
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5884..7062
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6115..7053
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     7010..7030
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      7111..7120
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7123..7158
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             7177..7218
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             7240..7281
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             7291..7998
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(7436..7454)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7704..7723
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    8068..8656
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8121..8141)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8539..8550)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             join(8731..8808,1..156)
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
ORIGIN
        1 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
       61 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
      121 tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta
      181 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg
      241 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
      301 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc
      361 tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
      421 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
      481 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat
      541 tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac
      601 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
      661 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
      721 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
      781 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
      841 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
      901 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
      961 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     1021 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     1081 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     1141 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc
     1201 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     1261 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     1321 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     1381 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     1441 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     1501 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     1561 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     1621 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     1681 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     1741 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     1801 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     1861 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     1921 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     1981 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     2041 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     2101 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     2161 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     2221 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     2281 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     2341 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     2401 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     2461 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     2521 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     2581 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     2641 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     2701 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     2761 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     2821 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     2881 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     2941 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     3001 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     3061 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     3121 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     3181 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     3241 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     3301 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa
     3361 ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt cttatgcaat
     3421 actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac aaggagagaa
     3481 aaagcaccgt gcatggcgat tggtggaagt aaggtggtac gatcgtgcct tattaggaag
     3541 gcaacagacg ggtctgacat ggattggacg aaccactgaa ttgccgcatt gcagagatat
     3601 tgtatttaag tgcctagctc gatacataaa cgggtctctc tggttagacc agatctgagc
     3661 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     3721 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     3781 acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg
     3841 aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca
     3901 agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag
     3961 gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa
     4021 aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca
     4081 agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt
     4141 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
     4201 ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc
     4261 aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa
     4321 gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt
     4381 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag
     4441 aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt
     4501 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca
     4561 attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca
     4621 gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt
     4681 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat
     4741 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg
     4801 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
     4861 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
     4921 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
     4981 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
     5041 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
     5101 cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga
     5161 cagatccatt cgattagtga acggatctcg acggtatcga tcacgagact agtatcgagc
     5221 ggccgccccc ttcaccgagg gcctatttcc catgattcct tcatatttgc atatacgata
     5281 caaggctgtt agagagataa ttggaattaa tttgactgta aacacaaaga tattagtaca
     5341 aaatacgtga cgtagaaagt aataatttct tgggtagttt gcagttttaa aattatgttt
     5401 taaaatggac tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct tggctttata
     5461 tatcttgtgg aaaggacgaa acaccgggtc ttcgagaaga cctgttttag agctagaaat
     5521 agcaagttaa aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct
     5581 ttttttaatt ctcgacctcg agacaaatgg cagtattcat ccacaatttt aaaagaaaag
     5641 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     5701 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     5761 acagcagaga tccactttgg ccgcggctcg aggcgataag ctttgcaaag atggataaag
     5821 ttttaaacag agaggaatct ttgcagctaa tggaccttct aggtcttgaa aggagtggga
     5881 attggctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg
     5941 gggggagggg tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa
     6001 agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt
     6061 gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggtaagt
     6121 gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt atggcccttg cgtgccttga
     6181 attacttcca cctggctgca gtacgtgatt cttgatcccg agcttcgggt tggaagtggg
     6241 tgggagagtt cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc
     6301 tggcctgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct
     6361 gctttcgata agtctctagc catttaaaat ttttgatgac ctgctgcgac gctttttttc
     6421 tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc ggtttttggg
     6481 gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg
     6541 cgagcgcggc caccgagaat cggacggggg tagtctcaag ctggccggcc tgctctggtg
     6601 cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg caaggctggc ccggtcggca
     6661 ccagttgcgt gagcggaaag atggccgctt cccggccctg ctgcagggag ctcaaaatgg
     6721 aggacgcggc gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa aagggccttt
     6781 ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc caggcacctc
     6841 gattagttct cgagcttttg gagtacgtcg tctttaggtt ggggggaggg gttttatgcg
     6901 atggagtttc cccacactga gtgggtggag actgaagtta ggccagcttg gcacttgatg
     6961 taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct caagcctcag
     7021 acagtggttc aaagtttttt tcttccattt caggtgtcgt gaggaattct gcagtcgatc
     7081 gacggtaccg cgggcccggg atccacggtc gccaccatgg gcgaggacca ggtggacccc
     7141 aggctgatcg acggcaaggg aggaggcggg ggagggagcg gcttcgccaa cgagctgggc
     7201 cccaggctga tgggcaagtg cggaggaggc gggggagggg gcaaaccaat tccaaaccca
     7261 ctgctgggac tggattccac aggcatgctg atggtgagca agggcgagga ggataacatg
     7321 gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt gaacggccac
     7381 gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag
     7441 ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc ccctcagttc
     7501 atgtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta cttgaagctg
     7561 tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg
     7621 accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt gaagctgcgc
     7681 ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg ctgggaggcc
     7741 tcctccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa gcagaggctg
     7801 aagctgaagg acggcggcca ctacgacgct gaggtcaaga ccacctacaa ggccaagaag
     7861 cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt tggacatcac ctcccacaac
     7921 gaggactaca ccatcgtgga acagtacgaa cgcgccgagg gccgccactc caccggcggc
     7981 atggacgagc tgtacaagaa acggccagcc gaggatatgg aggaagaaca agcctttaaa
     8041 cgatctcgat aaagcggccg cgtcgacaat caacctctgg attacaaaat ttgtgaaaga
     8101 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     8161 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     8221 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     8281 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     8341 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     8401 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     8461 aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     8521 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     8581 ccggctctgc ggcctcttcc gcatcttcgc cttcgccctc agacgagtcg gatctccctt
     8641 tgggccgcct ccccgcctgg gtacctttaa gaccaatgac ttacaaggca gctgtagatc
     8701 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagat
     8761 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatct
//
