LOCUS       sequence-353006-        8799 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables spatial detection of 
            CRISPR screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8799)
  AUTHORS   Dhainaut M, Rose SA, Akturk G, Wroblewska A, Nielsen SR, Park ES, 
            Buckup M, Roudko V, Pia L, Sweeney R, Le Berichel J, Wilk CM, 
            Bektesevic A, Lee BH, Bhardwaj N, Rahman AH, Baccarini A, Gnjatic S,
            Pe'er D, Merad M, Brown BD
  TITLE     Spatial CRISPR genomics identifies regulators of the tumor 
            microenvironment.
  JOURNAL   Cell. 2022 Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015.
  PUBMED    35290801
REFERENCE   2  (bases 1 to 8799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2022 
            Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015."
COMMENT     Sequence Label: pLKO_C.S.VSVg_mCherry-NLS
FEATURES             Location/Qualifiers
     source          1..8799
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(93..161,162..953)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(311..330)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1124..1712
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1613..1632
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1866..1883
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2000..2021
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2036..2041,2042..2059,2060..2066)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2074..2090
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2079..2101
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2098..2114
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2098..2114
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2133..2153
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2135..2153
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2181..2407
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2408..2588
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2635..2760
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3253..3486
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3671..3715
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        4013..4261
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     4013..4033
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     4184..4203
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        4280..4355
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    4403..4520
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4660..5838
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4891..5829
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5786..5806
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      5887..5896
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5899..5934
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             5953..5997
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             6016..6048
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     CDS             6058..6765
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6203..6221)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6471..6490
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    6835..7423
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6888..6908)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7306..7317)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7498..7731
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7803..7924
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7840..7859)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7894..7913
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7964..8099
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8119..8138)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8120..8138)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8148..8165)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8148..8164)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8157..8179)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8306..8761
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8393..8412)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8603..8624
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        join(8787..8799,1..92)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
       61 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
      121 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
      181 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
      241 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
      301 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
      361 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
      421 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
      481 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
      541 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
      601 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
      661 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
      721 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
      781 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
      841 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
      901 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
      961 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     1021 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     1081 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     1141 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     1201 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     1261 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     1321 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     1381 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     1441 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     1501 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     1561 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     1621 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     1681 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     1741 tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc cctgattctg
     1801 tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg
     1861 agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc
     1921 ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg
     1981 gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac
     2041 actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag
     2101 gaaacagcta tgaccatgat tacgccaagc gcgcaattaa ccctcactaa agggaacaaa
     2161 agctggagct gcaagcttaa tgtagtctta tgcaatactc ttgtagtctt gcaacatggt
     2221 aacgatgagt tagcaacatg ccttacaagg agagaaaaag caccgtgcat ggcgattggt
     2281 ggaagtaagg tggtacgatc gtgccttatt aggaaggcaa cagacgggtc tgacatggat
     2341 tggacgaacc actgaattgc cgcattgcag agatattgta tttaagtgcc tagctcgata
     2401 cataaacggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     2461 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     2521 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     2581 ctctagcagt ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct
     2641 cgacgcagga ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg
     2701 agtacgccaa aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc
     2761 agtattaagc gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga
     2821 aagaaaaaat ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca
     2881 gttaatcctg gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa
     2941 ccatcccttc agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc
     3001 tattgtgtgc atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag
     3061 gaagagcaaa acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg
     3121 aggaggagat atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat
     3181 tgaaccatta ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag
     3241 agcagtggga ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg
     3301 cgcagcgtca atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca
     3361 gcagaacaat ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg
     3421 gggcatcaag cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca
     3481 gctcctgggg atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa
     3541 tgctagttgg agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg
     3601 ggacagagaa attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa
     3661 ccagcaagaa aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa
     3721 ttggtttaac ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg
     3781 cttggtaggt ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg
     3841 atattcacca ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga
     3901 aggaatagaa gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg
     3961 atctcgacgg tatcgatcac gagactagta tcgagcggcc gcccccttca ccgagggcct
     4021 atttcccatg attccttcat atttgcatat acgatacaag gctgttagag agataattgg
     4081 aattaatttg actgtaaaca caaagatatt agtacaaaat acgtgacgta gaaagtaata
     4141 atttcttggg tagtttgcag ttttaaaatt atgttttaaa atggactatc atatgcttac
     4201 cgtaacttga aagtatttcg atttcttggc tttatatatc ttgtggaaag gacgaaacac
     4261 cgggtcttcg agaagacctg ttttagagct agaaatagca agttaaaata aggctagtcc
     4321 gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt ttaattctcg acctcgagac
     4381 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
     4441 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     4501 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca ctttggccgc
     4561 ggctcgaggc gataagcttt gcaaagatgg ataaagtttt aaacagagag gaatctttgc
     4621 agctaatgga ccttctaggt cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg
     4681 gcagagcgca catcgcccac agtccccgag aagttggggg gaggggtcgg caattgaacc
     4741 ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc
     4801 ctttttcccg agggtggggg agaaccgtat ataagtgcag tagtcgccgt gaacgttctt
     4861 tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct
     4921 ggcctcttta cgggttatgg cccttgcgtg ccttgaatta cttccacctg gctgcagtac
     4981 gtgattcttg atcccgagct tcgggttgga agtgggtggg agagttcgag gccttgcgct
     5041 taaggagccc cttcgcctcg tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc
     5101 gtgcgaatct ggtggcacct tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt
     5161 taaaattttt gatgacctgc tgcgacgctt tttttctggc aagatagtct tgtaaatgcg
     5221 ggccaagatc tgcacactgg tatttcggtt tttggggccg cgggcggcga cggggcccgt
     5281 gcgtcccagc gcacatgttc ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga
     5341 cgggggtagt ctcaagctgg ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc
     5401 gccccgccct gggcggcaag gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg
     5461 ccgcttcccg gccctgctgc agggagctca aaatggagga cgcggcgctc gggagagcgg
     5521 gcgggtgagt cacccacaca aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt
     5581 gactccacgg agtaccgggc gccgtccagg cacctcgatt agttctcgag cttttggagt
     5641 acgtcgtctt taggttgggg ggaggggttt tatgcgatgg agtttcccca cactgagtgg
     5701 gtggagactg aagttaggcc agcttggcac ttgatgtaat tctccttgga atttgccctt
     5761 tttgagtttg gatcttggtt cattctcaag cctcagacag tggttcaaag tttttttctt
     5821 ccatttcagg tgtcgtgagg aattctgcag tcgatcgacg gtaccgcggg cccgggatcc
     5881 acggtcgcca ccatgggcga ggaccaggtg gaccccaggc tgatcgacgg caagggagga
     5941 ggcgggggag ggaaggagac cgccgccgcc aagttcgaga ggcagcacat ggacagcgga
     6001 ggaggcgggg gagggtatac agatatcgaa atgaatagac tcggaaaggg catgctgatg
     6061 gtgagcaagg gcgaggagga taacatggcc atcatcaagg agttcatgcg cttcaaggtg
     6121 cacatggagg gctccgtgaa cggccacgag ttcgagatcg agggcgaggg cgagggccgc
     6181 ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc cctgcccttc
     6241 gcctgggaca tcctgtcccc tcagttcatg tacggctcca aggcctacgt gaagcacccc
     6301 gccgacatcc ccgactactt gaagctgtcc ttccccgagg gcttcaagtg ggagcgcgtg
     6361 atgaacttcg aggacggcgg cgtggtgacc gtgacccagg actcctccct gcaggacggc
     6421 gagttcatct acaaggtgaa gctgcgcggc accaacttcc cctccgacgg ccccgtaatg
     6481 cagaagaaga ccatgggctg ggaggcctcc tccgagcgga tgtaccccga ggacggcgcc
     6541 ctgaagggcg agatcaagca gaggctgaag ctgaaggacg gcggccacta cgacgctgag
     6601 gtcaagacca cctacaaggc caagaagccc gtgcagctgc ccggcgccta caacgtcaac
     6661 atcaagttgg acatcacctc ccacaacgag gactacacca tcgtggaaca gtacgaacgc
     6721 gccgagggcc gccactccac cggcggcatg gacgagctgt acaagaaacg gccagccgag
     6781 gatatggagg aagaacaagc ctttaaacga tctcgataaa gcggccgcgt cgacaatcaa
     6841 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     6901 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     6961 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     7021 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     7081 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     7141 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     7201 actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt
     7261 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     7321 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgca tcttcgcctt
     7381 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcctgggta cctttaagac
     7441 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     7501 aagggctaat tcactcccaa cgaagataag atctgctttt tgcttgtact gggtctctct
     7561 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     7621 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     7681 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtagtagtt
     7741 catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca gagagtgaga
     7801 ggaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     7861 caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     7921 cttatcatgt ctggctctag ctatcccgcc cctaactccg cccatcccgc ccctaactcc
     7981 gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     8041 cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     8101 agggacgtac ccaattcgcc ctatagtgag tcgtattacg cgcgctcact ggccgtcgtt
     8161 ttacaacgtc gtgactggga aaaccctggc gttacccaac ttaatcgcct tgcagcacat
     8221 ccccctttcg ccagctggcg taatagcgaa gaggcccgca ccgatcgccc ttcccaacag
     8281 ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg gcgcattaag cgcggcgggt
     8341 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     8401 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     8461 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     8521 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     8581 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     8641 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     8701 aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt
     8761 taggtggcac ttttcgggga aatgtgcgcg gaaccccta
//
