LOCUS       sequence-353010-        8796 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables spatial detection of 
            CRISPR screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8796)
  AUTHORS   Dhainaut M, Rose SA, Akturk G, Wroblewska A, Nielsen SR, Park ES, 
            Buckup M, Roudko V, Pia L, Sweeney R, Le Berichel J, Wilk CM, 
            Bektesevic A, Lee BH, Bhardwaj N, Rahman AH, Baccarini A, Gnjatic S,
            Pe'er D, Merad M, Brown BD
  TITLE     Spatial CRISPR genomics identifies regulators of the tumor 
            microenvironment.
  JOURNAL   Cell. 2022 Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015.
  PUBMED    35290801
REFERENCE   2  (bases 1 to 8796)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2022 
            Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015."
COMMENT     Sequence Label: pLKO_C.VSVg.V5_mCherry-NLS
FEATURES             Location/Qualifiers
     source          1..8796
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        122..226
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(227..295,296..1087)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(445..464)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1258..1846
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1747..1766
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2000..2017
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2134..2155
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2170..2175,2176..2193,2194..2200)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2208..2224
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2213..2235
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2232..2248
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2232..2248
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2267..2287
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2269..2287
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2315..2541
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2542..2722
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2769..2894
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3387..3620
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3805..3849
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        4147..4395
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     4147..4167
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     4318..4337
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        4414..4489
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    4537..4654
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4794..5972
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5025..5963
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5920..5940
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      6021..6030
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6033..6068
                     /codon_start=1
                     /product="epitope tag derived from human Protein C (Stearns
                     et al., 1988)"
                     /label=Protein C tag
                     /translation="EDQVDPRLIDGK"
     CDS             6087..6119
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     CDS             6138..6179
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6189..6896
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6334..6352)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6602..6621
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    6966..7554
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7019..7039)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7437..7448)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7629..7862
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7934..8055
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7971..7990)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8025..8044
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8095..8230
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8250..8269)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8251..8269)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8279..8296)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8279..8295)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8288..8310)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      join(8437..8796,1..96)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8524..8543)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8734..8755
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
       61 acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt
      121 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
      181 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
      241 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
      301 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
      361 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
      421 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
      481 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
      541 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
      601 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
      661 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
      721 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
      781 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
      841 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
      901 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
      961 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     1021 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     1081 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     1141 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     1201 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     1261 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     1321 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     1381 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     1441 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     1501 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     1561 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     1621 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     1681 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     1741 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     1801 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     1861 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt
     1921 atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg
     1981 cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg
     2041 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     2101 cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     2161 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     2221 acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa ttaaccctca
     2281 ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt cttatgcaat actcttgtag
     2341 tcttgcaaca tggtaacgat gagttagcaa catgccttac aaggagagaa aaagcaccgt
     2401 gcatggcgat tggtggaagt aaggtggtac gatcgtgcct tattaggaag gcaacagacg
     2461 ggtctgacat ggattggacg aaccactgaa ttgccgcatt gcagagatat tgtatttaag
     2521 tgcctagctc gatacataaa cgggtctctc tggttagacc agatctgagc ctgggagctc
     2581 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
     2641 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
     2701 tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac
     2761 cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg
     2821 ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg
     2881 ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt
     2941 taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc
     3001 tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac
     3061 tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata
     3121 cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt
     3181 tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg
     3241 atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat
     3301 aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg
     3361 cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca
     3421 ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct
     3481 ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg
     3541 caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac
     3601 ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact
     3661 gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg
     3721 acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt
     3781 gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg
     3841 gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa attattcata
     3901 atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat
     3961 agagttaggc agggatattc accattatcg tttcagaccc acctcccaac cccgagggga
     4021 cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt
     4081 cgattagtga acggatctcg acggtatcga tcacgagact agtatcgagc ggccgccccc
     4141 ttcaccgagg gcctatttcc catgattcct tcatatttgc atatacgata caaggctgtt
     4201 agagagataa ttggaattaa tttgactgta aacacaaaga tattagtaca aaatacgtga
     4261 cgtagaaagt aataatttct tgggtagttt gcagttttaa aattatgttt taaaatggac
     4321 tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct tggctttata tatcttgtgg
     4381 aaaggacgaa acaccgggtc ttcgagaaga cctgttttag agctagaaat agcaagttaa
     4441 aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct ttttttaatt
     4501 ctcgacctcg agacaaatgg cagtattcat ccacaatttt aaaagaaaag gggggattgg
     4561 ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac aaactaaaga
     4621 attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg acagcagaga
     4681 tccactttgg ccgcggctcg aggcgataag ctttgcaaag atggataaag ttttaaacag
     4741 agaggaatct ttgcagctaa tggaccttct aggtcttgaa aggagtggga attggctccg
     4801 gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg gggggagggg
     4861 tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg
     4921 tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt gcagtagtcg
     4981 ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg
     5041 gttcccgcgg gcctggcctc tttacgggtt atggcccttg cgtgccttga attacttcca
     5101 cctggctgca gtacgtgatt cttgatcccg agcttcgggt tggaagtggg tgggagagtt
     5161 cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc
     5221 gctggggccg ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct gctttcgata
     5281 agtctctagc catttaaaat ttttgatgac ctgctgcgac gctttttttc tggcaagata
     5341 gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc ggtttttggg gccgcgggcg
     5401 gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg cgagcgcggc
     5461 caccgagaat cggacggggg tagtctcaag ctggccggcc tgctctggtg cctggcctcg
     5521 cgccgccgtg tatcgccccg ccctgggcgg caaggctggc ccggtcggca ccagttgcgt
     5581 gagcggaaag atggccgctt cccggccctg ctgcagggag ctcaaaatgg aggacgcggc
     5641 gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa aagggccttt ccgtcctcag
     5701 ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc caggcacctc gattagttct
     5761 cgagcttttg gagtacgtcg tctttaggtt ggggggaggg gttttatgcg atggagtttc
     5821 cccacactga gtgggtggag actgaagtta ggccagcttg gcacttgatg taattctcct
     5881 tggaatttgc cctttttgag tttggatctt ggttcattct caagcctcag acagtggttc
     5941 aaagtttttt tcttccattt caggtgtcgt gaggaattct gcagtcgatc gacggtaccg
     6001 cgggcccggg atccacggtc gccaccatgg gcgaggacca ggtggacccc aggctgatcg
     6061 acggcaaggg aggaggcggg ggagggtata cagatatcga aatgaataga ctcggaaagg
     6121 gaggaggcgg gggagggggc aaaccaattc caaacccact gctgggactg gattccacag
     6181 gcatgctgat ggtgagcaag ggcgaggagg ataacatggc catcatcaag gagttcatgc
     6241 gcttcaaggt gcacatggag ggctccgtga acggccacga gttcgagatc gagggcgagg
     6301 gcgagggccg cccctacgag ggcacccaga ccgccaagct gaaggtgacc aagggtggcc
     6361 ccctgccctt cgcctgggac atcctgtccc ctcagttcat gtacggctcc aaggcctacg
     6421 tgaagcaccc cgccgacatc cccgactact tgaagctgtc cttccccgag ggcttcaagt
     6481 gggagcgcgt gatgaacttc gaggacggcg gcgtggtgac cgtgacccag gactcctccc
     6541 tgcaggacgg cgagttcatc tacaaggtga agctgcgcgg caccaacttc ccctccgacg
     6601 gccccgtaat gcagaagaag accatgggct gggaggcctc ctccgagcgg atgtaccccg
     6661 aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact
     6721 acgacgctga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct
     6781 acaacgtcaa catcaagttg gacatcacct cccacaacga ggactacacc atcgtggaac
     6841 agtacgaacg cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagaaac
     6901 ggccagccga ggatatggag gaagaacaag cctttaaacg atctcgataa agcggccgcg
     6961 tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     7021 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     7081 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg
     7141 agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc
     7201 ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc
     7261 tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc
     7321 ggctgttggg cactgacaat tccgtggtgt tgtcggggaa gctgacgtcc tttccatggc
     7381 tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg
     7441 ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc
     7501 atcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcctgggt
     7561 acctttaaga ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa
     7621 ggggggactg gaagggctaa ttcactccca acgaagataa gatctgcttt ttgcttgtac
     7681 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     7741 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     7801 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     7861 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc
     7921 agagagtgag aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat
     7981 cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact
     8041 catcaatgta tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg
     8101 cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt
     8161 tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt
     8221 tttggaggcc tagggacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac
     8281 tggccgtcgt tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc
     8341 ttgcagcaca tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc
     8401 cttcccaaca gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa
     8461 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
     8521 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
     8581 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
     8641 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
     8701 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
     8761 cactcaaccc tatctcggtc tattcttttg atttat
//
