LOCUS       sequence-353013-        8790 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables spatial detection of 
            CRISPR screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8790)
  AUTHORS   Dhainaut M, Rose SA, Akturk G, Wroblewska A, Nielsen SR, Park ES, 
            Buckup M, Roudko V, Pia L, Sweeney R, Le Berichel J, Wilk CM, 
            Bektesevic A, Lee BH, Bhardwaj N, Rahman AH, Baccarini A, Gnjatic S,
            Pe'er D, Merad M, Brown BD
  TITLE     Spatial CRISPR genomics identifies regulators of the tumor 
            microenvironment.
  JOURNAL   Cell. 2022 Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015.
  PUBMED    35290801
REFERENCE   2  (bases 1 to 8790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2022 
            Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015."
COMMENT     Sequence Label: pLKO_HA.HSV.Ollas_mCherry-NLS
FEATURES             Location/Qualifiers
     source          1..8790
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        77..181
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(182..250,251..1042)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(400..419)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1213..1801
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1702..1721
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1955..1972
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2089..2110
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2125..2130,2131..2148,2149..2155)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2163..2179
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2168..2190
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2187..2203
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2187..2203
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2222..2242
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2224..2242
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2270..2496
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2497..2677
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2724..2849
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3342..3575
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3760..3804
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        4102..4350
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     4102..4122
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     4273..4292
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        4369..4444
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    4492..4609
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4749..5927
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4980..5918
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5875..5895
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      5976..5985
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5988..6014
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6033..6065
                     /codon_start=1
                     /product="HSV (herpes simplex virus) epitope tag"
                     /label=HSV tag
                     /translation="QPELAPEDPED"
     CDS             6084..6125
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             6138..6845
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6283..6301)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6551..6570
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    6915..7503
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6968..6988)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7386..7397)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7578..7811
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7883..8004
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7920..7939)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7974..7993
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8044..8179
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8199..8218)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8200..8218)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8228..8245)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8228..8244)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8237..8259)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      join(8386..8790,1..51)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8473..8492)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8683..8704
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 tttaacaaaa atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac
       61 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
      121 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
      181 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
      241 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
      301 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
      361 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
      421 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
      481 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
      541 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
      601 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
      661 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
      721 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
      781 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
      841 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
      901 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
      961 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
     1021 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
     1081 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
     1141 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
     1201 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
     1261 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
     1321 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
     1381 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
     1441 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
     1501 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
     1561 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
     1621 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
     1681 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
     1741 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
     1801 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
     1861 gttctttcct gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc
     1921 tgataccgct cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga
     1981 agagcgccca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg
     2041 gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta
     2101 gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg
     2161 aattgtgagc ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg
     2221 cgcaattaac cctcactaaa gggaacaaaa gctggagctg caagcttaat gtagtcttat
     2281 gcaatactct tgtagtcttg caacatggta acgatgagtt agcaacatgc cttacaagga
     2341 gagaaaaagc accgtgcatg gcgattggtg gaagtaaggt ggtacgatcg tgccttatta
     2401 ggaaggcaac agacgggtct gacatggatt ggacgaacca ctgaattgcc gcattgcaga
     2461 gatattgtat ttaagtgcct agctcgatac ataaacgggt ctctctggtt agaccagatc
     2521 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     2581 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     2641 ctcagaccct tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga
     2701 aagcgaaagg gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca
     2761 cggcaagagg cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct
     2821 agaaggagag agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat
     2881 gggaaaaaat tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat
     2941 gggcaagcag ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag
     3001 gctgtagaca aatactggga cagctacaac catcccttca gacaggatca gaagaactta
     3061 gatcattata taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag
     3121 acaccaagga agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac
     3181 agcaagcggc cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt
     3241 gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca
     3301 aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg
     3361 ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc
     3421 agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg
     3481 caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg
     3541 gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa
     3601 ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag
     3661 atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta
     3721 atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg
     3781 gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat
     3841 ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta
     3901 ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc
     3961 ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac
     4021 agagacagat ccattcgatt agtgaacgga tctcgacggt atcgatcacg agactagtat
     4081 cgagcggccg cccccttcac cgagggccta tttcccatga ttccttcata tttgcatata
     4141 cgatacaagg ctgttagaga gataattgga attaatttga ctgtaaacac aaagatatta
     4201 gtacaaaata cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta
     4261 tgttttaaaa tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct
     4321 ttatatatct tgtggaaagg acgaaacacc gggtcttcga gaagacctgt tttagagcta
     4381 gaaatagcaa gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg
     4441 gtgctttttt taattctcga cctcgagaca aatggcagta ttcatccaca attttaaaag
     4501 aaaagggggg attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga
     4561 catacaaact aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta
     4621 cagggacagc agagatccac tttggccgcg gctcgaggcg ataagctttg caaagatgga
     4681 taaagtttta aacagagagg aatctttgca gctaatggac cttctaggtc ttgaaaggag
     4741 tgggaattgg ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga
     4801 agttgggggg aggggtcggc aattgaaccg gtgcctagag aaggtggcgc ggggtaaact
     4861 gggaaagtga tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata
     4921 taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg
     4981 taagtgccgt gtgtggttcc cgcgggcctg gcctctttac gggttatggc ccttgcgtgc
     5041 cttgaattac ttccacctgg ctgcagtacg tgattcttga tcccgagctt cgggttggaa
     5101 gtgggtggga gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt gcttgagttg
     5161 aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc
     5221 tcgctgcttt cgataagtct ctagccattt aaaatttttg atgacctgct gcgacgcttt
     5281 ttttctggca agatagtctt gtaaatgcgg gccaagatct gcacactggt atttcggttt
     5341 ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg
     5401 gcctgcgagc gcggccaccg agaatcggac gggggtagtc tcaagctggc cggcctgctc
     5461 tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt
     5521 cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca gggagctcaa
     5581 aatggaggac gcggcgctcg ggagagcggg cgggtgagtc acccacacaa aggaaaaggg
     5641 cctttccgtc ctcagccgtc gcttcatgtg actccacgga gtaccgggcg ccgtccaggc
     5701 acctcgatta gttctcgagc ttttggagta cgtcgtcttt aggttggggg gaggggtttt
     5761 atgcgatgga gtttccccac actgagtggg tggagactga agttaggcca gcttggcact
     5821 tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg atcttggttc attctcaagc
     5881 ctcagacagt ggttcaaagt ttttttcttc catttcaggt gtcgtgagga attctgcagt
     5941 cgatcgacgg taccgcgggc ccgggatcca cggtcgccac catgggctac ccatacgatg
     6001 tgccagatta cgctggagga ggcgggggag ggcagcccga gctggccccc gaggaccccg
     6061 aggacggagg aggcggggga gggagcggct tcgccaacga gctgggcccc aggctgatgg
     6121 gcaagtgcgg catgctgatg gtgagcaagg gcgaggagga taacatggcc atcatcaagg
     6181 agttcatgcg cttcaaggtg cacatggagg gctccgtgaa cggccacgag ttcgagatcg
     6241 agggcgaggg cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca
     6301 agggtggccc cctgcccttc gcctgggaca tcctgtcccc tcagttcatg tacggctcca
     6361 aggcctacgt gaagcacccc gccgacatcc ccgactactt gaagctgtcc ttccccgagg
     6421 gcttcaagtg ggagcgcgtg atgaacttcg aggacggcgg cgtggtgacc gtgacccagg
     6481 actcctccct gcaggacggc gagttcatct acaaggtgaa gctgcgcggc accaacttcc
     6541 cctccgacgg ccccgtaatg cagaagaaga ccatgggctg ggaggcctcc tccgagcgga
     6601 tgtaccccga ggacggcgcc ctgaagggcg agatcaagca gaggctgaag ctgaaggacg
     6661 gcggccacta cgacgctgag gtcaagacca cctacaaggc caagaagccc gtgcagctgc
     6721 ccggcgccta caacgtcaac atcaagttgg acatcacctc ccacaacgag gactacacca
     6781 tcgtggaaca gtacgaacgc gccgagggcc gccactccac cggcggcatg gacgagctgt
     6841 acaagaaacg gccagccgag gatatggagg aagaacaagc ctttaaacga tctcgataaa
     6901 gcggccgcgt cgacaatcaa cctctggatt acaaaatttg tgaaagattg actggtattc
     6961 ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct ttgtatcatg
     7021 ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg ttgctgtctc
     7081 tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg
     7141 acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc gggactttcg
     7201 ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga
     7261 caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct
     7321 ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc ttctgctacg
     7381 tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg gctctgcggc
     7441 ctcttccgca tcttcgcctt cgccctcaga cgagtcggat ctccctttgg gccgcctccc
     7501 cgcctgggta cctttaagac caatgactta caaggcagct gtagatctta gccacttttt
     7561 aaaagaaaag gggggactgg aagggctaat tcactcccaa cgaagataag atctgctttt
     7621 tgcttgtact gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     7681 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     7741 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     7801 aatctctagc agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa
     7861 atgaatatca gagagtgaga ggaacttgtt tattgcagct tataatggtt acaaataaag
     7921 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     7981 gtccaaactc atcaatgtat cttatcatgt ctggctctag ctatcccgcc cctaactccg
     8041 cccatcccgc ccctaactcc gcccagttcc gcccattctc cgccccatgg ctgactaatt
     8101 ttttttattt atgcagaggc cgaggccgcc tcggcctctg agctattcca gaagtagtga
     8161 ggaggctttt ttggaggcct agggacgtac ccaattcgcc ctatagtgag tcgtattacg
     8221 cgcgctcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
     8281 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
     8341 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgggacgcg ccctgtagcg
     8401 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     8461 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     8521 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     8581 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     8641 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     8701 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     8761 tttcggccta ttggttaaaa aatgagctga
//
