LOCUS       sequence-353014-        8790 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables spatial detection of 
            CRISPR screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8790)
  AUTHORS   Dhainaut M, Rose SA, Akturk G, Wroblewska A, Nielsen SR, Park ES, 
            Buckup M, Roudko V, Pia L, Sweeney R, Le Berichel J, Wilk CM, 
            Bektesevic A, Lee BH, Bhardwaj N, Rahman AH, Baccarini A, Gnjatic S,
            Pe'er D, Merad M, Brown BD
  TITLE     Spatial CRISPR genomics identifies regulators of the tumor 
            microenvironment.
  JOURNAL   Cell. 2022 Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015.
  PUBMED    35290801
REFERENCE   2  (bases 1 to 8790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2022 
            Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015."
COMMENT     Sequence Label: pLKO_HA.HSV.S_mCherry-NLS
FEATURES             Location/Qualifiers
     source          1..8790
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        126..230
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(231..299,300..1091)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(449..468)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1262..1850
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1751..1770
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2004..2021
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2138..2159
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2174..2179,2180..2197,2198..2204)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2212..2228
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2217..2239
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2236..2252
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2236..2252
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2271..2291
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2273..2291
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2319..2545
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2546..2726
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2773..2898
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3391..3624
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3809..3853
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        4151..4399
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     4151..4171
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     4322..4341
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        4418..4493
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    4541..4658
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4798..5976
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5029..5967
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5924..5944
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      6025..6034
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6037..6063
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6082..6114
                     /codon_start=1
                     /product="HSV (herpes simplex virus) epitope tag"
                     /label=HSV tag
                     /translation="QPELAPEDPED"
     CDS             6133..6177
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             6187..6894
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6332..6350)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6600..6619
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    6964..7552
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7017..7037)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7435..7446)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7627..7860
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7932..8053
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7969..7988)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8023..8042
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8093..8228
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8248..8267)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8249..8267)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8277..8294)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8277..8293)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8286..8308)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      join(8435..8790,1..100)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8522..8541)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8732..8753
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
       61 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa
      121 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
      181 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
      241 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
      301 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
      361 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
      421 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
      481 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
      541 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
      601 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
      661 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
      721 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
      781 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
      841 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
      901 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
      961 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     1021 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     1081 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     1141 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     1201 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     1261 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     1321 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     1381 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     1441 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     1501 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     1561 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     1621 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     1681 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     1741 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     1801 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     1861 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     1921 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     1981 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     2041 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     2101 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
     2161 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     2221 gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc
     2281 ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg caatactctt
     2341 gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag agaaaaagca
     2401 ccgtgcatgg cgattggtgg aagtaaggtg gtacgatcgt gccttattag gaaggcaaca
     2461 gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag atattgtatt
     2521 taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct gagcctggga
     2581 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     2641 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     2701 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg
     2761 aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc
     2821 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga
     2881 gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt
     2941 cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg
     3001 gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa
     3061 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat
     3121 aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa
     3181 gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc
     3241 gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg aattatataa
     3301 atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt
     3361 ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc
     3421 agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt
     3481 gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct
     3541 gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag
     3601 atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac
     3661 cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca
     3721 cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt
     3781 aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa
     3841 atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt
     3901 cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt
     3961 gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag
     4021 gggacccgac aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc
     4081 cattcgatta gtgaacggat ctcgacggta tcgatcacga gactagtatc gagcggccgc
     4141 ccccttcacc gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc
     4201 tgttagagag ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac
     4261 gtgacgtaga aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat
     4321 ggactatcat atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt
     4381 gtggaaagga cgaaacaccg ggtcttcgag aagacctgtt ttagagctag aaatagcaag
     4441 ttaaaataag gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcttttttt
     4501 aattctcgac ctcgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga
     4561 ttggggggta cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta
     4621 aagaattaca aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca
     4681 gagatccact ttggccgcgg ctcgaggcga taagctttgc aaagatggat aaagttttaa
     4741 acagagagga atctttgcag ctaatggacc ttctaggtct tgaaaggagt gggaattggc
     4801 tccggtgccc gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga
     4861 ggggtcggca attgaaccgg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat
     4921 gtcgtgtact ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta
     4981 gtcgccgtga acgttctttt tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg
     5041 tgtggttccc gcgggcctgg cctctttacg ggttatggcc cttgcgtgcc ttgaattact
     5101 tccacctggc tgcagtacgt gattcttgat cccgagcttc gggttggaag tgggtgggag
     5161 agttcgaggc cttgcgctta aggagcccct tcgcctcgtg cttgagttga ggcctggcct
     5221 gggcgctggg gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc
     5281 gataagtctc tagccattta aaatttttga tgacctgctg cgacgctttt tttctggcaa
     5341 gatagtcttg taaatgcggg ccaagatctg cacactggta tttcggtttt tggggccgcg
     5401 ggcggcgacg gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg
     5461 cggccaccga gaatcggacg ggggtagtct caagctggcc ggcctgctct ggtgcctggc
     5521 ctcgcgccgc cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt
     5581 gcgtgagcgg aaagatggcc gcttcccggc cctgctgcag ggagctcaaa atggaggacg
     5641 cggcgctcgg gagagcgggc gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc
     5701 tcagccgtcg cttcatgtga ctccacggag taccgggcgc cgtccaggca cctcgattag
     5761 ttctcgagct tttggagtac gtcgtcttta ggttgggggg aggggtttta tgcgatggag
     5821 tttccccaca ctgagtgggt ggagactgaa gttaggccag cttggcactt gatgtaattc
     5881 tccttggaat ttgccctttt tgagtttgga tcttggttca ttctcaagcc tcagacagtg
     5941 gttcaaagtt tttttcttcc atttcaggtg tcgtgaggaa ttctgcagtc gatcgacggt
     6001 accgcgggcc cgggatccac ggtcgccacc atgggctacc catacgatgt gccagattac
     6061 gctggaggag gcgggggagg gcagcccgag ctggcccccg aggaccccga ggacggagga
     6121 ggcgggggag ggaaggagac cgccgccgcc aagttcgaga ggcagcacat ggacagcggc
     6181 atgctgatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc
     6241 ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc
     6301 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc
     6361 ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg
     6421 aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg
     6481 gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg
     6541 caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc
     6601 cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag
     6661 gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac
     6721 gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac
     6781 aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag
     6841 tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta caagaaacgg
     6901 ccagccgagg atatggagga agaacaagcc tttaaacgat ctcgataaag cggccgcgtc
     6961 gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     7021 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     7081 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     7141 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     7201 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     7261 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     7321 ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg
     7381 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     7441 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcat
     7501 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctgggtac
     7561 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     7621 ggggactgga agggctaatt cactcccaac gaagataaga tctgcttttt gcttgtactg
     7681 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     7741 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     7801 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     7861 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
     7921 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     7981 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     8041 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc
     8101 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     8161 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     8221 tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg
     8281 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     8341 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     8401 tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc
     8461 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     8521 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     8581 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     8641 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     8701 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     8761 ctcaacccta tctcggtcta ttcttttgat
//
