LOCUS       sequence-353015-        9817 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9817)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9817)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1583
FEATURES             Location/Qualifiers
     source          1..9817
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    2755..3343
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2808..2828)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3226..3237)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3415..3648
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3726..3847
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3763..3782)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3817..3836
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3887..4022
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4042..4061)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4043..4061)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4071..4088)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4071..4087)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4080..4102)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4229..4684
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4316..4335)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4526..4547
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4710..4814
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4815..4883,4884..5675)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5033..5052)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5846..6434
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6335..6354
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6588..6605
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6722..6743
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6758..6763,6764..6781,6782..6788)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6796..6812
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6801..6823
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6820..6836
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6820..6836
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6855..6875
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6857..6875
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6903..7129
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7130..7310
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7357..7482
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7975..8208
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8393..8437
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8668..8878
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8690..8886
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8737..8872
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8918..9520
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8918..8937)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9414..9434
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9563..9680
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgtg
     1201 gctggggact tcagggaaga gtgggttacc tggacactgc ttagagaatc ctctccagga
     1261 atgccaccca gcacagttag aagaatgggc tctcaaagga atttccaggc ctagtgtaat
     1321 ctcccagccg gagcagaaag aagagccatg ggtcctacca ctccaaaact ttgaggcgag
     1381 gaagatcccg agggaaagcc acacagactg tgagcatcag gtggcaaagc tcaatcagga
     1441 caattctgaa acagcagaac aatgtggaac atcctcagaa aggaccaata aagatctttc
     1501 tcatactctt agttggggag gaaactggga gcaaggccta gaattagaag ggcaacatgg
     1561 aacccttcca ggagagggcc agctggagtc cttttcacag gagagggatt taaacaagct
     1621 cctggatgga tatgtaggag agaagcctat gtgtgcagaa tgcgggaaaa gctttaacca
     1681 gagttcctat ctcataagac acctaagaac ccacactggc gagaggccct atacgtgcat
     1741 tgagtgtggg aaaggcttca aacagagctc agaccttgtc acccatcgca gaacacacac
     1801 aggagagaag ccctaccaat gcaaggggtg tgagaagaaa ttcagcgaca gctcaacact
     1861 catcaaacat cagagaaccc acacagggga gagaccctat gagtgcccag agtgtggaaa
     1921 gacttttggg cggaagccac acctcataat gcaccaaaga acccacacag gcgagaagcc
     1981 ctacgcgtgc ctggaatgtc acaaaagctt cagtcgaagc tcaaatttca tcactcacca
     2041 gaggacccac acaggggtga agccttacag gtgtaatgac tgtggggaga gttttagcca
     2101 gagctcggat ttgattaagc accaacgaac ccacacggga gaacggccct tcaaatgccc
     2161 ggagtgcggg aagggcttca gagatagttc tcattttgta gctcacatga gcactcattc
     2221 aggagagagg cctttcagtt gtcctgactg ccacaaaagc ttcagtcaga gctcacattt
     2281 ggtcacgcac caaagaacac acacaggtga gagacctttt aagtgcgaaa actgtgggaa
     2341 aggattcgcc gacagctccg ccctcattaa gcaccaacga atccacaccg gagaaagacc
     2401 ctacaaatgt ggagagtgtg ggaagagctt caatcagagc tcccacttta ttacccatca
     2461 gcgaatccac ttaggagaca ggccctatcg atgtcctgag tgtggcaaga ccttcaatca
     2521 gcgttcccat ttcctcacac accagagaac gcatacagga gaaaaacctt tccactgtag
     2581 taaatgtaac aagagcttcc gtcagaaagc gcatctttta tgccatcaaa acacccattt
     2641 gatttagtaa tgaactagtc cgtaacttga aagtatttcg atttcttggc tttatatatc
     2701 ttgtggaaag gacgacaacg taatgcggat ccaccacgca cgcgttaagt cgacaatcaa
     2761 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     2821 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     2881 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     2941 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     3001 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     3061 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     3121 actgacaatt ccgtggtgtt gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt
     3181 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     3241 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt
     3301 cgccctcaga cgagtcggat ctccctttgg gccgcctccc cgcgtcgact ttaagaccaa
     3361 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     3421 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt
     3481 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     3541 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     3601 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt acgtatagta
     3661 gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata tcagagagtg
     3721 agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
     3781 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     3841 tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc cgcccctaac
     3901 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     3961 ggccgaggcc gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     4021 cctagggacg tacccaattc gccctatagt gagtcgtatt acgcgcgctc actggccgtc
     4081 gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca
     4141 catccccctt tcgccagctg gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa
     4201 cagttgcgca gcctgaatgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg
     4261 ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca gcgccctagc gcccgctcct
     4321 ttcgctttct tcccttcctt tctcgccacg ttcgccggct ttccccgtca agctctaaat
     4381 cgggggctcc ctttagggtt ccgatttagt gctttacggc acctcgaccc caaaaaactt
     4441 gattagggtg atggttcacg tagtgggcca tcgccctgat agacggtttt tcgccctttg
     4501 acgttggagt ccacgttctt taatagtgga ctcttgttcc aaactggaac aacactcaac
     4561 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
     4621 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
     4681 atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     4741 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     4801 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     4861 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     4921 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     4981 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     5041 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     5101 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     5161 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     5221 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     5281 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     5341 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     5401 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     5461 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     5521 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     5581 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     5641 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     5701 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     5761 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     5821 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     5881 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     5941 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     6001 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     6061 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     6121 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     6181 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     6241 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     6301 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     6361 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     6421 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     6481 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     6541 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     6601 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     6661 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     6721 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     6781 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
     6841 attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt
     6901 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
     6961 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
     7021 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
     7081 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
     7141 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     7201 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     7261 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
     7321 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
     7381 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
     7441 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
     7501 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
     7561 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
     7621 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
     7681 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
     7741 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
     7801 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
     7861 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     7921 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     7981 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     8041 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     8101 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     8161 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     8221 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     8281 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     8341 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     8401 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     8461 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     8521 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     8581 tcagacccac ctcccaaccc cgaggggacc catgcattgc atctcaatta gtcagcaacc
     8641 aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcgtctcaat
     8701 tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     8761 tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     8821 gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     8881 tgcaaaaagc tttctagagg taccaccgga gcttaccatg accgagtaca agcccacggt
     8941 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc
     9001 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga
     9061 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
     9121 cgacggcgcc gccgtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
     9181 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
     9241 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
     9301 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
     9361 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
     9421 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
     9481 gcgcacctgg tgcatgaccc gcaagcccgg tgccagttaa cgaattctag atcttgagac
     9541 aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg tacagtgcag
     9601 gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta caaaaacaaa
     9661 ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagagatcca ctttggcgcc
     9721 ggctcgaggc ctgcaggtgc aaagatggat aaagttttaa acagagagga atctttgcag
     9781 ctaatggacc ttctaggtct tgaaaggagt gggaatt
//
