LOCUS       sequence-353016-        8778 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables spatial detection of 
            CRISPR screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8778)
  AUTHORS   Dhainaut M, Rose SA, Akturk G, Wroblewska A, Nielsen SR, Park ES, 
            Buckup M, Roudko V, Pia L, Sweeney R, Le Berichel J, Wilk CM, 
            Bektesevic A, Lee BH, Bhardwaj N, Rahman AH, Baccarini A, Gnjatic S,
            Pe'er D, Merad M, Brown BD
  TITLE     Spatial CRISPR genomics identifies regulators of the tumor 
            microenvironment.
  JOURNAL   Cell. 2022 Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015.
  PUBMED    35290801
REFERENCE   2  (bases 1 to 8778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2022 
            Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015."
COMMENT     Sequence Label: pLKO_HA.HSV.VSVg_mCherry-NLS
FEATURES             Location/Qualifiers
     source          1..8778
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    97..118
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(133..138,139..156,157..163)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    171..187
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     176..198
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     195..211
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     195..211
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     230..250
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        232..250
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        278..504
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             505..685
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    732..857
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1350..1583
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1768..1812
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2110..2358
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2110..2130
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2281..2300
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        2377..2452
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    2500..2617
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2757..3935
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2988..3926
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3883..3903
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      3984..3993
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             3996..4022
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4041..4073
                     /codon_start=1
                     /product="HSV (herpes simplex virus) epitope tag"
                     /label=HSV tag
                     /translation="QPELAPEDPED"
     CDS             4092..4124
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     CDS             4134..4841
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4279..4297)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4547..4566
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    4911..5499
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4964..4984)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5382..5393)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5574..5807
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5879..6000
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5916..5935)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5970..5989
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6040..6175
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6195..6214)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6196..6214)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6224..6241)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6224..6240)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6233..6255)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6382..6837
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6469..6488)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6679..6700
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6863..6967
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6968..7036,7037..7828)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7186..7205)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7999..8587
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8488..8507
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8741..8758
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
       61 tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
      121 ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
      181 ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg caattaaccc
      241 tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc aatactcttg
      301 tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga gaaaaagcac
      361 cgtgcatggc gattggtgga agtaaggtgg tacgatcgtg ccttattagg aaggcaacag
      421 acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga tattgtattt
      481 aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg agcctgggag
      541 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
      601 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
      661 tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga
      721 aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg
      781 aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag aaggagagag
      841 atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg gaaaaaattc
      901 ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg gcaagcaggg
      961 agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc tgtagacaaa
     1021 tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga tcattatata
     1081 atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac accaaggaag
     1141 ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag caagcggccg
     1201 ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga attatataaa
     1261 tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa gagaagagtg
     1321 gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt cttgggagca
     1381 gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag acaattattg
     1441 tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca acagcatctg
     1501 ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc tgtggaaaga
     1561 tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact catttgcacc
     1621 actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat ttggaatcac
     1681 acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat acactcctta
     1741 attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga attagataaa
     1801 tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat aaaattattc
     1861 ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact ttctatagtg
     1921 aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc aaccccgagg
     1981 ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag agacagatcc
     2041 attcgattag tgaacggatc tcgacggtat cgatcacgag actagtatcg agcggccgcc
     2101 cccttcaccg agggcctatt tcccatgatt ccttcatatt tgcatatacg atacaaggct
     2161 gttagagaga taattggaat taatttgact gtaaacacaa agatattagt acaaaatacg
     2221 tgacgtagaa agtaataatt tcttgggtag tttgcagttt taaaattatg ttttaaaatg
     2281 gactatcata tgcttaccgt aacttgaaag tatttcgatt tcttggcttt atatatcttg
     2341 tggaaaggac gaaacaccgg gtcttcgaga agacctgttt tagagctaga aatagcaagt
     2401 taaaataagg ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gcttttttta
     2461 attctcgacc tcgagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
     2521 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
     2581 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
     2641 agatccactt tggccgcggc tcgaggcgat aagctttgca aagatggata aagttttaaa
     2701 cagagaggaa tctttgcagc taatggacct tctaggtctt gaaaggagtg ggaattggct
     2761 ccggtgcccg tcagtgggca gagcgcacat cgcccacagt ccccgagaag ttggggggag
     2821 gggtcggcaa ttgaaccggt gcctagagaa ggtggcgcgg ggtaaactgg gaaagtgatg
     2881 tcgtgtactg gctccgcctt tttcccgagg gtgggggaga accgtatata agtgcagtag
     2941 tcgccgtgaa cgttcttttt cgcaacgggt ttgccgccag aacacaggta agtgccgtgt
     3001 gtggttcccg cgggcctggc ctctttacgg gttatggccc ttgcgtgcct tgaattactt
     3061 ccacctggct gcagtacgtg attcttgatc ccgagcttcg ggttggaagt gggtgggaga
     3121 gttcgaggcc ttgcgcttaa ggagcccctt cgcctcgtgc ttgagttgag gcctggcctg
     3181 ggcgctgggg ccgccgcgtg cgaatctggt ggcaccttcg cgcctgtctc gctgctttcg
     3241 ataagtctct agccatttaa aatttttgat gacctgctgc gacgcttttt ttctggcaag
     3301 atagtcttgt aaatgcgggc caagatctgc acactggtat ttcggttttt ggggccgcgg
     3361 gcggcgacgg ggcccgtgcg tcccagcgca catgttcggc gaggcggggc ctgcgagcgc
     3421 ggccaccgag aatcggacgg gggtagtctc aagctggccg gcctgctctg gtgcctggcc
     3481 tcgcgccgcc gtgtatcgcc ccgccctggg cggcaaggct ggcccggtcg gcaccagttg
     3541 cgtgagcgga aagatggccg cttcccggcc ctgctgcagg gagctcaaaa tggaggacgc
     3601 ggcgctcggg agagcgggcg ggtgagtcac ccacacaaag gaaaagggcc tttccgtcct
     3661 cagccgtcgc ttcatgtgac tccacggagt accgggcgcc gtccaggcac ctcgattagt
     3721 tctcgagctt ttggagtacg tcgtctttag gttgggggga ggggttttat gcgatggagt
     3781 ttccccacac tgagtgggtg gagactgaag ttaggccagc ttggcacttg atgtaattct
     3841 ccttggaatt tgcccttttt gagtttggat cttggttcat tctcaagcct cagacagtgg
     3901 ttcaaagttt ttttcttcca tttcaggtgt cgtgaggaat tctgcagtcg atcgacggta
     3961 ccgcgggccc gggatccacg gtcgccacca tgggctaccc atacgatgtg ccagattacg
     4021 ctggaggagg cgggggaggg cagcccgagc tggcccccga ggaccccgag gacggaggag
     4081 gcgggggagg gtatacagat atcgaaatga atagactcgg aaagggcatg ctgatggtga
     4141 gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca
     4201 tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct
     4261 acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct
     4321 gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg
     4381 acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga
     4441 acttcgagga cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt
     4501 tcatctacaa ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga
     4561 agaagaccat gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga
     4621 agggcgagat caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca
     4681 agaccaccta caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca
     4741 agttggacat cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg
     4801 agggccgcca ctccaccggc ggcatggacg agctgtacaa gaaacggcca gccgaggata
     4861 tggaggaaga acaagccttt aaacgatctc gataaagcgg ccgcgtcgac aatcaacctc
     4921 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     4981 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     5041 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     5101 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     5161 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     5221 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     5281 acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg
     5341 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     5401 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcatctt cgccttcgcc
     5461 ctcagacgag tcggatctcc ctttgggccg cctccccgcc tgggtacctt taagaccaat
     5521 gacttacaag gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg
     5581 gctaattcac tcccaacgaa gataagatct gctttttgct tgtactgggt ctctctggtt
     5641 agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca
     5701 ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa
     5761 ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg
     5821 tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa
     5881 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
     5941 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
     6001 tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc
     6061 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     6121 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggg
     6181 acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac
     6241 aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     6301 ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     6361 gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg
     6421 tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt
     6481 tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc
     6541 tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg
     6601 gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg
     6661 agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct
     6721 cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg ttaaaaaatg
     6781 agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt acaatttagg
     6841 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     6901 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     6961 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     7021 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     7081 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     7141 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     7201 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     7261 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     7321 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     7381 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     7441 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     7501 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     7561 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     7621 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     7681 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     7741 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     7801 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     7861 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     7921 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     7981 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     8041 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     8101 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     8161 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     8221 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     8281 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     8341 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     8401 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     8461 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     8521 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     8581 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     8641 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga
     8701 gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga
     8761 agcggaagag cgcccaat
//
