LOCUS       sequence-35302-e         887 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: HL 3152 (=HL 716 + pHL 1056)
FEATURES             Location/Qualifiers
     source          1..887
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    214..230
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        288..392
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(611..630)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 gttcttctcc tttacgcatg ctatcatgaa ctttcagcgt attctgactc ataaggtggc
       61 tcctacccgt gatcccttga cggaacattc aagcaaaagc ctggttccgc cgatttatcg
      121 ctgcggcaaa taacgcagcg ggtttacgga tttccccttg taacgaattt caaaatgcaa
      181 gcgtgttgaa ctggttccgg tgtcgacagt atcttgttat ccgctcacaa tgtcaatgtt
      241 atccgctcac atttatgacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
      301 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
      361 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
      421 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
      481 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
      541 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
      601 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
      661 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
      721 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
      781 acactgcggc caacttactt ctgacaacga tcggaggacc gaaaggagct aaccgctttt
      841 ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaacc
//
