LOCUS       sequence-353020-        8784 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables spatial detection of 
            CRISPR screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8784)
  AUTHORS   Dhainaut M, Rose SA, Akturk G, Wroblewska A, Nielsen SR, Park ES, 
            Buckup M, Roudko V, Pia L, Sweeney R, Le Berichel J, Wilk CM, 
            Bektesevic A, Lee BH, Bhardwaj N, Rahman AH, Baccarini A, Gnjatic S,
            Pe'er D, Merad M, Brown BD
  TITLE     Spatial CRISPR genomics identifies regulators of the tumor 
            microenvironment.
  JOURNAL   Cell. 2022 Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015.
  PUBMED    35290801
REFERENCE   2  (bases 1 to 8784)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2022 
            Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015."
COMMENT     Sequence Label: pLKO_HA.NWS.Ollas_mCherry-NLS
FEATURES             Location/Qualifiers
     source          1..8784
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     210..227
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    344..365
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(380..385,386..403,404..410)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    418..434
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     423..445
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     442..458
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     442..458
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     477..497
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        479..497
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        525..751
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             752..932
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    979..1104
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1597..1830
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2015..2059
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        2357..2605
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2357..2377
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2528..2547
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        2624..2699
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    2747..2864
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        3004..4182
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          3235..4173
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     4130..4150
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      4231..4240
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4243..4269
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4291..4314
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             4333..4374
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             4387..5094
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(4532..4550)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     4800..4819
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    5164..5752
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5217..5237)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5635..5646)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5827..6060
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6132..6253
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6169..6188)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6223..6242
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6293..6428
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6448..6467)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6449..6467)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6477..6494)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6477..6493)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6486..6508)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6635..7090
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6722..6741)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6932..6953
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7116..7220
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7221..7289,7290..8081)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7439..7458)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(8252..8784,1..56)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8741..8760
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
       61 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
      121 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
      181 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
      241 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
      301 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
      361 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
      421 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa
      481 ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt cttatgcaat
      541 actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac aaggagagaa
      601 aaagcaccgt gcatggcgat tggtggaagt aaggtggtac gatcgtgcct tattaggaag
      661 gcaacagacg ggtctgacat ggattggacg aaccactgaa ttgccgcatt gcagagatat
      721 tgtatttaag tgcctagctc gatacataaa cgggtctctc tggttagacc agatctgagc
      781 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
      841 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
      901 acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg
      961 aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca
     1021 agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag
     1081 gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa
     1141 aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca
     1201 agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt
     1261 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
     1321 ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc
     1381 aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa
     1441 gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt
     1501 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag
     1561 aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt
     1621 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca
     1681 attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca
     1741 gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt
     1801 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat
     1861 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg
     1921 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
     1981 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
     2041 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
     2101 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
     2161 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
     2221 cccgagggga cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga
     2281 cagatccatt cgattagtga acggatctcg acggtatcga tcacgagact agtatcgagc
     2341 ggccgccccc ttcaccgagg gcctatttcc catgattcct tcatatttgc atatacgata
     2401 caaggctgtt agagagataa ttggaattaa tttgactgta aacacaaaga tattagtaca
     2461 aaatacgtga cgtagaaagt aataatttct tgggtagttt gcagttttaa aattatgttt
     2521 taaaatggac tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct tggctttata
     2581 tatcttgtgg aaaggacgaa acaccgggtc ttcgagaaga cctgttttag agctagaaat
     2641 agcaagttaa aataaggcta gtccgttatc aacttgaaaa agtggcaccg agtcggtgct
     2701 ttttttaatt ctcgacctcg agacaaatgg cagtattcat ccacaatttt aaaagaaaag
     2761 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2821 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2881 acagcagaga tccactttgg ccgcggctcg aggcgataag ctttgcaaag atggataaag
     2941 ttttaaacag agaggaatct ttgcagctaa tggaccttct aggtcttgaa aggagtggga
     3001 attggctccg gtgcccgtca gtgggcagag cgcacatcgc ccacagtccc cgagaagttg
     3061 gggggagggg tcggcaattg aaccggtgcc tagagaaggt ggcgcggggt aaactgggaa
     3121 agtgatgtcg tgtactggct ccgccttttt cccgagggtg ggggagaacc gtatataagt
     3181 gcagtagtcg ccgtgaacgt tctttttcgc aacgggtttg ccgccagaac acaggtaagt
     3241 gccgtgtgtg gttcccgcgg gcctggcctc tttacgggtt atggcccttg cgtgccttga
     3301 attacttcca cctggctgca gtacgtgatt cttgatcccg agcttcgggt tggaagtggg
     3361 tgggagagtt cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc
     3421 tggcctgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct
     3481 gctttcgata agtctctagc catttaaaat ttttgatgac ctgctgcgac gctttttttc
     3541 tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc ggtttttggg
     3601 gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg
     3661 cgagcgcggc caccgagaat cggacggggg tagtctcaag ctggccggcc tgctctggtg
     3721 cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg caaggctggc ccggtcggca
     3781 ccagttgcgt gagcggaaag atggccgctt cccggccctg ctgcagggag ctcaaaatgg
     3841 aggacgcggc gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa aagggccttt
     3901 ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc caggcacctc
     3961 gattagttct cgagcttttg gagtacgtcg tctttaggtt ggggggaggg gttttatgcg
     4021 atggagtttc cccacactga gtgggtggag actgaagtta ggccagcttg gcacttgatg
     4081 taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct caagcctcag
     4141 acagtggttc aaagtttttt tcttccattt caggtgtcgt gaggaattct gcagtcgatc
     4201 gacggtaccg cgggcccggg atccacggtc gccaccatgg gctacccata cgatgtgcca
     4261 gattacgctg gaggaggcgg gggagggaac tggtcccacc cccaattcga gaaaggagga
     4321 ggcgggggag ggagcggctt cgccaacgag ctgggcccca ggctgatggg caagtgcggc
     4381 atgctgatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc
     4441 ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc
     4501 gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc
     4561 ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg
     4621 aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg
     4681 gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg
     4741 caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc
     4801 cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag
     4861 gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac
     4921 gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac
     4981 aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag
     5041 tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta caagaaacgg
     5101 ccagccgagg atatggagga agaacaagcc tttaaacgat ctcgataaag cggccgcgtc
     5161 gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     5221 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     5281 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     5341 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     5401 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     5461 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     5521 ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg
     5581 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     5641 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcat
     5701 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctgggtac
     5761 ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg
     5821 ggggactgga agggctaatt cactcccaac gaagataaga tctgcttttt gcttgtactg
     5881 ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac
     5941 tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt
     6001 gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca
     6061 gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
     6121 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     6181 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     6241 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc
     6301 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     6361 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     6421 tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg
     6481 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     6541 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     6601 tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc
     6661 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     6721 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     6781 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     6841 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     6901 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     6961 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     7021 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     7081 cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     7141 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     7201 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     7261 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     7321 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     7381 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     7441 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     7501 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     7561 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     7621 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     7681 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     7741 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     7801 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     7861 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     7921 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     7981 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     8041 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     8101 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     8161 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     8221 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     8281 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     8341 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     8401 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     8461 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     8521 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     8581 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     8641 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     8701 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     8761 atagtcctgt cgggtttcgc cacc
//
