LOCUS       sequence-353025-        2605 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2605)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2605)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-AC2
FEATURES             Location/Qualifiers
     source          1..2605
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     243..262
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(470..475,476..492,493..498)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             546..1736
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(898..917)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(1910..1929)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1911..1929)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1934..1950)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1934..1950)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      join(2359..2605,1..343)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
       61 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
      121 gaactgagat acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag
      181 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
      241 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
      301 cgatttttgt gatgctcgtc agggggggcg gagcctatgg aaaaacgcca gcaacgcggc
      361 ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
      421 cacagaatca ggggataacg caggaaagaa catgaattaa ttctcatgtt tgacagctta
      481 tcatcgatta gctttaatgc ggtagtttat cacagttaaa ttgctaacgc agtcaggcac
      541 cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg caccgtcacc ctggatgctg
      601 taggcatagg cttggttatg ccggtactgc cgggcctctt gcgggatatc gtccattccg
      661 acagcatcgc cagtcactat ggcgtgctgc tagcgctata tgcgttgatg caatttctat
      721 gcgcacccgt tctcggagca ctgtccgacc gctttggccg ccgcccagtc ctgctcgctt
      781 cgctacttgg agccactatc gactacgcga tcatggcgac cacacccgtc ctgtggattc
      841 tctacgccgg acgcatcgtg gccggcatca ccggcgccac aggtgcggtt gctggcgcct
      901 atatcgccga catcaccgat ggggaagatc gggctcgcca cttcgggctc atgagcgctt
      961 gtttcggcgt gggtatggtg gcaggccccg tggccggggg actgttgggc gccatctcct
     1021 tacatgcacc attccttgcg gcggcggtgc tcaacggcct caacctacta ctgggctgct
     1081 tcctaatgca ggagtcgcat aagggagagc gccgacccat gcccttgaga gccttcaacc
     1141 cagtcagctc cttccggtgg gcgcggggca tgactatcgt cgccgcactt atgactgtct
     1201 tctttatcat gcaactcgta ggacaggtgc cggcagcgct ctgggtcatt ttcggcgagg
     1261 accgctttcg ctggagcgcg acgatgatcg gcctgtcgct tgcggtattc ggaatcttgc
     1321 acgccctcgc tcaagccttc gtcactggtc ccgccaccaa acgtttcggc gagaagcagg
     1381 ccattatcgc cggcatggcg gccgacgcgc tgggctacgt cttgctggcg ttcgcgacgc
     1441 gaggctggat ggccttcccc attatgattc ttctcgcttc cggcggcatc gggatgcccg
     1501 cgttgcaggc catgctgtcc aggcaggtag atgacgacca tcagggacag cttcaaggat
     1561 cgctcgcggc tcttaccagc ctaacttcga tcattggacc gctgatcgtc acggcgattt
     1621 atgccgcctc ggcgagcaca tggaacgggt tggcatggat tgtaggcgcc gccctatacc
     1681 ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg ggccacctcg acctgaatgg
     1741 aagccggcgg cacctcgcta acggattcac cactccaaga attggagcca atcaattctt
     1801 gcggagaact gtgaatgcgc aaaccaaccc ttgatcgggg aagaacagta tgtcgagcta
     1861 ttttttgact tactggggat caagcctgat tgggagaaaa taaaatatcc cctatagtga
     1921 gtcgtattac atggtcatag ctgtttcctg gcagctctgg cccgtgtctc aaaatctctg
     1981 atgttacatt gcacaagata aaaatatatc atcatgcctc ctctagacca aagctggtct
     2041 caaccacttg cgctggggtt agtccgtggg cttgacagag aactggtaga aggcgttgga
     2101 cggtttcaag agcctgtttg ccaccgtcat gactagcaat cgctacaact tgatctgggg
     2161 tgagtccgtg ggcttgacag agaactggaa ggaggcgctg aaccgtctca agagcctgtt
     2221 tgccaccaat attactagca atcgctacaa cctgcgagac catatctggt cgagccaccc
     2281 atgaccaaaa tcccttaacg tgagttacgc gtcgttccac tgagcgtcag accccgtaga
     2341 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     2401 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     2461 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     2521 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     2581 cctgttacca gtggctgctg ccagt
//
