LOCUS       sequence-353027-       10843 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10843)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF1634
FEATURES             Location/Qualifiers
     source          1..10843
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1190..1199
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(2608..2619)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     RBS             3036..3044
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     misc_feature    3781..4369
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3834..3854)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4252..4263)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4441..4674
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4752..4873
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4789..4808)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4843..4862
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4913..5048
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5068..5087)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5069..5087)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5097..5114)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5097..5113)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5106..5128)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5255..5710
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5342..5361)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5552..5573
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5736..5840
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5841..5909,5910..6701)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6059..6078)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6872..7460
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7361..7380
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7614..7631
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7748..7769
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7784..7789,7790..7807,7808..7814)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7822..7838
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7827..7849
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7846..7862
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7846..7862
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7881..7901
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7883..7901
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7929..8155
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8156..8336
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8383..8508
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9001..9234
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9419..9463
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9694..9904
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9716..9912
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9763..9898
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9944..10546
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9944..9963)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     10440..10460
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10589..10706
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgga
     1201 gactttgacc tcaaggcatg agaaaagagc tcttcattct caagcctcag ccatttccca
     1261 agatagggag gagaagatca tgtctcagga gccattgagc ttcaaggacg tggctgtggt
     1321 cttcactgag gaggagctag agctgctgga ctctacccag aggcagctgt accaagatgt
     1381 gatgcaggag aatttcagga acctactctc agtgggtgag aggaatcctc tgggagacaa
     1441 gaatggaaag gatacggagt atattcaaga tgaagaatta aggttctttt cacacaaaga
     1501 gctctcctca tgcaaaatct gggaagaggt ggcaggtgaa ttacctggga gccaagactg
     1561 tagagtaaat ctgcaaggaa aagacttcca gttctcagaa gatgctgctc cccatcaagg
     1621 gtgggaagga gcatctacgc cgtgttttcc aattgagaat ttcctggaca gtctacaagg
     1681 ggatgggctt atcggtctag aaaatcaaca gtttccagcc tggagagcta taagaccaat
     1741 ccccattcaa ggatcttggg caaaagcgtt tgtgaaccag ttaggggatg ttcaagaaag
     1801 atgtaaaaat ctcgacacag aagacacagt atataaatgt aactgggatg atgacagctt
     1861 ttgctggata tcttgtcatg ttgatcacag attccctgaa atagacaagc cgtgtggttg
     1921 caataaatgc agaaaagact gcattaaaaa ctctgtactt catcgcatta accctggaga
     1981 gaatggcttg aaaagtaacg aatacagaaa tggcttcagg gacgatgcag accttccccc
     2041 gcatccaaga gtacctttga aagagaaact ctgtcaatat gatgagttta gtgagggctt
     2101 gaggcacagt gcccatctta acagacatca aagagttccc acaggagaga aatctgttaa
     2161 gagtcttgag cgtggtcggg gcgtcagaca gaacacgcac atacgtaacc accccagagc
     2221 ccctgtggga gacatgccct atagatgtga tgtctgtgga aaggggttca ggtataaatc
     2281 ggttcttctt attcatcaag gggtgcacac aggaaggaga ccctataaat gtgaggagtg
     2341 tgggaaggca tttggtcgaa gttcaaacct gcttgtccat cagagggtcc acactggaga
     2401 gaaaccatat aaatgcagcg agtgtgggaa gggcttcagt tacagctcag tgcttcaagt
     2461 ccatcagagg ctgcacacag gggagaagcc ctacacctgc agcgagtgtg gcaaaggctt
     2521 ctgtgccaag tctgcactgc acaaacacca gcacattcac cctggagaaa agccctacag
     2581 ctgtggcgag tgtggaaagg gattcagctg cagctcccac ctcagcagtc atcagaagac
     2641 acacaccggc gagaggccct accagtgtga caagtgtggc aaaggtttca gtcacaactc
     2701 gtaccttcaa gctcaccaga gagttcacat ggggcagcat ctgtacaaat gtaacgtgtg
     2761 tggtaagagt ttcagttaca gctcagggct tctcatgcat cagagactgc acacaggaga
     2821 gaaaccctac aaatgcgagt gcgggaagag ctttggccgg agctccgacc tccacatcca
     2881 tcagagggtc cacacaggag agaaacccta taaatgcagt gagtgtggga agggcttccg
     2941 gcggaattca gaccttcaca gccaccagag ggtccacacg ggagagaggc cctacgtgtg
     3001 tgacgtgtgt gggaagggtt tcatctacag ctccgacctc cttatccatc agagggtcca
     3061 cactggagag aaaccctata aatgtgctga gtgtggcaaa ggcttcagtt acagctcagg
     3121 gcttctcatt caccagagag tccacacagg cgagaaacct tacagatgcc aagagtgcgg
     3181 aaagggcttt aggtgcacat caagccttca caaacatcag cgagtccaca cgggaaaaaa
     3241 gccctatacg tgtgatcagt gtggcaaggg attcagttat ggctctaatc ttcgcaccca
     3301 ccagaggttg cacacaggag agaaacccta cacttgttgt gaatgtggga agggtttcag
     3361 atatggctca ggtctcctta gtcataagag agtgcacact ggcgagaagc catacagatg
     3421 ccacgtgtgt gggaagggct atagtcagag ctcacatctt caaggtcatc agagggtcca
     3481 cactggtgag aaaccctata aatgtgagga gtgtgggaag ggctttggcc gcaactcctg
     3541 tcttcatgtt catcagagag tccacactgg agagaagccc tatacgtgtg gtgtgtgtgg
     3601 gaaaggcttc agttatacct caggtctgcg gaaccaccaa agagtgcatt taggcgagaa
     3661 cccttataag tagtaatgaa ctagtccgta acttgaaagt atttcgattt cttggcttta
     3721 tatatcttgt ggaaaggacg aaaacaggag caatttcatc agacgacgcg ttaagtcgac
     3781 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     3841 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     3901 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     3961 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     4021 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     4081 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     4141 ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc ttggctgctc
     4201 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     4261 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     4321 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcg tcgactttaa
     4381 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     4441 tggaagggct aattcactcc caacgaagac aagatctgct ttttgcttgt actgggtctc
     4501 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     4561 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     4621 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtacgt
     4681 atagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag
     4741 agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     4801 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     4861 tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc
     4921 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
     4981 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
     5041 tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg
     5101 gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt
     5161 gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct
     5221 tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc
     5281 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     5341 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     5401 ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     5461 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     5521 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     5581 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     5641 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     5701 cttacaattt aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     5761 tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     5821 aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     5881 ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     5941 ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     6001 tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     6061 tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac
     6121 actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     6181 gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     6241 acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     6301 gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     6361 acgagcgtga caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg
     6421 gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     6481 ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     6541 gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     6601 cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     6661 agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     6721 catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     6781 tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     6841 cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     6901 gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     6961 taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc
     7021 ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     7081 tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     7141 ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     7201 cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     7261 agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     7321 gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     7381 atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     7441 gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     7501 gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     7561 ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     7621 cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     7681 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca
     7741 acgcaattaa tgtgagttag ctcactcatt aggcacccca ggctttacac tttatgcttc
     7801 cggctcgtat gttgtgtgga attgtgagcg gataacaatt tcacacagga aacagctatg
     7861 accatgatta cgccaagcgc gcaattaacc ctcactaaag ggaacaaaag ctggagctgc
     7921 aagcttaatg tagtcttatg caatactctt gtagtcttgc aacatggtaa cgatgagtta
     7981 gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgc cgattggtgg aagtaaggtg
     8041 gtacgatcgt gccttattag gaaggcaaca gacgggtctg acatggattg gacgaaccac
     8101 tgaattgccg cattgcagag atattgtatt taagtgccta gctcgataca taaacgggtc
     8161 tctctggtta gaccagatct gagcctggga gctctctggc taactaggga acccactgct
     8221 taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga
     8281 ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtgg
     8341 cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg agctctctcg acgcaggact
     8401 cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc gactggtgag tacgccaaaa
     8461 attttgacta gcggaggcta gaaggagaga gatgggtgcg agagcgtcag tattaagcgg
     8521 gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc cagggggaaa gaaaaaatat
     8581 aaattaaaac atatagtatg ggcaagcagg gagctagaac gattcgcagt taatcctggc
     8641 ctgttagaaa catcagaagg ctgtagacaa atactgggac agctacaacc atcccttcag
     8701 acaggatcag aagaacttag atcattatat aatacagtag caaccctcta ttgtgtgcat
     8761 caaaggatag agataaaaga caccaaggaa gctttagaca agatagagga agagcaaaac
     8821 aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc agacctggag gaggagatat
     8881 gagggacaat tggagaagtg aattatataa atataaagta gtaaaaattg aaccattagg
     8941 agtagcaccc accaaggcaa agagaagagt ggtgcagaga gaaaaaagag cagtgggaat
     9001 aggagctttg ttccttgggt tcttgggagc agcaggaagc actatgggcg cagcgtcaat
     9061 gacgctgacg gtacaggcca gacaattatt gtctggtata gtgcagcagc agaacaattt
     9121 gctgagggct attgaggcgc aacagcatct gttgcaactc acagtctggg gcatcaagca
     9181 gctccaggca agaatcctgg ctgtggaaag atacctaaag gatcaacagc tcctggggat
     9241 ttggggttgc tctggaaaac tcatttgcac cactgctgtg ccttggaatg ctagttggag
     9301 taataaatct ctggaacaga tttggaatca cacgacctgg atggagtggg acagagaaat
     9361 taacaattac acaagcttaa tacactcctt aattgaagaa tcgcaaaacc agcaagaaaa
     9421 gaatgaacaa gaattattgg aattagataa atgggcaagt ttgtggaatt ggtttaacat
     9481 aacaaattgg ctgtggtata taaaattatt cataatgata gtaggaggct tggtaggttt
     9541 aagaatagtt tttgctgtac tttctatagt gaatagagtt aggcagggat attcaccatt
     9601 atcgtttcag acccacctcc caaccccgag gggacccatg cattgcatct caattagtca
     9661 gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcgt
     9721 ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg
     9781 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     9841 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     9901 ggcttttgca aaaagctttc tagaggtacc accggagctt accatgaccg agtacaagcc
     9961 cacggtgcgc ctcgccaccc gcgacgacgt ccccagggcc gtacgcaccc tcgccgccgc
    10021 gttcgccgac taccccgcca cgcgccacac cgtcgatccg gaccgccaca tcgagcgggt
    10081 caccgagctg caagaactct tcctcacgcg cgtcgggctc gacatcggca aggtgtgggt
    10141 cgcggacgac ggcgccgccg tggcggtctg gaccacgccg gagagcgtcg aagcgggggc
    10201 ggtgttcgcc gagatcggcc cgcgcatggc cgagttgagc ggttcccggc tggccgcgca
    10261 gcaacagatg gaaggcctcc tggcgccgca ccggcccaag gagcccgcgt ggttcctggc
    10321 caccgtcggc gtctcgcccg accaccaggg caagggtctg ggcagcgccg tcgtgctccc
    10381 cggagtggag gcggccgagc gcgccggggt gcccgccttc ctggagacct ccgcgccccg
    10441 caacctcccc ttctacgagc ggctcggctt caccgtcacc gccgacgtcg aggtgcccga
    10501 aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc agttaacgaa ttctagatct
    10561 tgagacaaat ggcagtattc atccacaatt ttaaaagaaa aggggggatt ggggggtaca
    10621 gtgcagggga aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa
    10681 aacaaattac aaaaattcaa aattttcggg tttattacag ggacagcaga gatccacttt
    10741 ggcgccggct cgaggcctgc aggtgcaaag atggataaag ttttaaacag agaggaatct
    10801 ttgcagctaa tggaccttct aggtcttgaa aggagtggga att
//
