LOCUS       sequence-353030-       14047 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14047)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 14047)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2325
FEATURES             Location/Qualifiers
     source          1..14047
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1190..1199
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             complement(2769..2783)
                     /codon_start=1
                     /product="tag for protein cleavage using Ni2+ ion (Dang et 
                     al., 2019)"
                     /label=SNAC tag
                     /translation="GSHHW"
     misc_feature    6985..7573
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7038..7058)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7456..7467)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7645..7878
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7956..8077
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7993..8012)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8047..8066
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8117..8252
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8272..8291)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8273..8291)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8301..8318)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8301..8317)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8310..8332)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8459..8914
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8546..8565)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8756..8777
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        8940..9044
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9045..9113,9114..9905)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9263..9282)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10076..10664
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10565..10584
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     10818..10835
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    10952..10973
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(10988..10993,10994..11011,11012..11018)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11026..11042
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11031..11053
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11050..11066
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11050..11066
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11085..11105
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11087..11105
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        11133..11359
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             11360..11540
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    11587..11712
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    12205..12438
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             12623..12667
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        12898..13108
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        12920..13116
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      12967..13102
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             13148..13750
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(13148..13167)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     13644..13664
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    13793..13910
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatggg
     1201 acccggctgc gatttgctgc tgcggacagc agctaccatc actgctgccg ccatcatgtc
     1261 agacacggac agcgacgaag attccgctgg aggcggccca ttttctttag cgggtttcct
     1321 tttcggcaac atcaatggag ccgggcagct ggagggggaa agcgtcttgg atgatgaatg
     1381 taagaagcac ttggcaggct tgggggcttt ggggctgggc agcctgatca ctgaactcac
     1441 ggcaaatgaa gaattgaccg ggactgacgg tgccttggta aatgatgaag ggtgggttag
     1501 gagtacagaa gatgctgtgg actattcaga catcaatgag gtggcagaag atgaaagccg
     1561 aagataccag cagacgatgg ggagcttgca gcccctttgc cactcagatt atgatgaaga
     1621 tgactatgat gctgattgtg aagacattga ttgcaagttg atgcctcctc cacctccacc
     1681 cccgggacca atgaagaagg ataaggacca ggattctatt actggtgtgt ctgaaaatgg
     1741 agaaggcatc atcttgccct ccatcattgc cccttcctct ttggcctcag agaaagtgga
     1801 cttcagtagt tcctctgact cagaatctga gatgggacct caggaagcaa cacaggcaga
     1861 atctgaagat ggaaagctga cccttccatt ggctgggatt atgcagcatg atgccaccaa
     1921 gctgttgcca agtgtcacag aactttttcc agaatttcga cctggaaagg tgttacgttt
     1981 tctacgtctt tttggaccag ggaagaatgt cccatctgtt tggcggagtg ctcggagaaa
     2041 gaggaagaag aagcaccgtg agctgataca ggaagagcag atccaggagg tggagtgctc
     2101 agtagaatca gaagtcagcc agaagtcttt gtggaactac gactacgctc caccaccacc
     2161 tccagagcag tgtctctctg atgatgaaat cacgatgatg gctcctgtgg agtccaaatt
     2221 ttcccaatca actggagata tagataaagt gacagatacc aaaccaagag tggctgagtg
     2281 gcgttatggg cctgcccgac tgtggtatga tatgctgggt gtccctgaag atggcagtgg
     2341 gtttgactat ggcttcaaac tgagaaagac agaacatgaa cctgtgataa aatctagaat
     2401 gatagaggaa tttaggaaac ttgaggaaaa caatggcact gatcttctgg ctgatgaaaa
     2461 cttcctgatg gtgacacagc tgcattggga ggatgatatc atctgggatg gggaggatgt
     2521 caaacacaaa gggacaaaac ctcagcgtgc aagcctggca ggctggcttc cttctagcat
     2581 gactaggaat gcgatggctt acaatgttca gcaaggtttt gcagccactc ttgatgatga
     2641 caaaccttgg tactccattt ttcccattga caatgaggat ctggtatatg gacgctggga
     2701 ggacaatatc atttgggatg ctcaggccat gccccggctg ttggaacctc ctgttttgac
     2761 acttgatccc aatgatgaga acctcatttt ggaaattcct gatgagaagg aagaggccac
     2821 ctctaactcc ccctccaagg agagtaagaa ggaatcatct ctgaagaaga gtcgaattct
     2881 cttagggaaa acaggagtca tcaaggagga accacagcag aacatgtctc agccagaagt
     2941 gaaagatcca tggaatctct ccaatgatga gtattattat cccaagcaac agggtcttcg
     3001 aggcaccttt ggagggaata ttatccagca ttcaattcct gctgtggaat tacggcagcc
     3061 cttctttccc acccacatgg ggcccatcaa actccggcag ttccatcgcc cacctctgaa
     3121 aaagtactca tttggtgcac tttctcagcc aggtccccac tcagtccaac ctttgctaaa
     3181 gcacatcaaa aaaaaggcca agatgagaga acaagagagg caagcttcag gtggtggaga
     3241 gatgtttttt atgcgcacac ctcaggacct cacaggcaaa gatggtgatc ttattcttgc
     3301 agaatatagt gaggaaaatg gacccttaat gatgcaggtt ggcatggcaa ccaagataaa
     3361 gaactattat aaacggaaac ctggaaaaga tcctggagca ccagattgta aatatgggga
     3421 aactgtttac tgccatacat ctcctttcct gggttctctc catcctggcc aattgctgca
     3481 agcatttgag aacaaccttt ttcgtgctcc aatttatctt cataagatgc cagaaactga
     3541 tttcttgatc attcggacaa gacagggtta ctatattcgg gaattagtgg atatttttgt
     3601 ggttggccag cagtgtccct tgtttgaagt tcctgggcct aactccaaaa gggccaatac
     3661 gcatattcga gactttctac aggtttttat ttaccgcctt ttctggaaaa gtaaagatcg
     3721 gccacggagg atacgaatgg aagatataaa aaaagccttt ccttcccatt cagaaagcag
     3781 catccggaag aggctaaagc tctgcgctga cttcaaacgc acagggatgg actcaaactg
     3841 gtgggtgctt aagtctgatt ttcgtttacc aacggaagaa gagatcagag ctatggtgtc
     3901 accagagcag tgctgtgctt attatagcat gatagctgca gagcaacgac tgaaggatgc
     3961 tggctatggt gagaaatcct tttttgctcc agaagaagaa aatgaggaag atttccagat
     4021 gaagattgat gatgaagttc gcactgcccc ttggaacacc acaagggcct tcattgctgc
     4081 catgaagggc aagtgtctgc tagaggtgac tggggtggca gatcccacgg ggtgtggtga
     4141 aggattctcc tatgtgaaga ttccaaacaa accaacacag cagaaggatg ataaagaacc
     4201 gcagccagtg aagaagacag tgacaggaac agatgcagac cttcgtcgcc tttccctgaa
     4261 aaatgccaag caacttctac gtaaatttgg tgtgcctgag gaagagatta aaaagttgtc
     4321 ccgctgggaa gtgattgatg tggtgcgcac aatgtcaaca gaacaggctc gttctggaga
     4381 ggggcccatg agtaaatttg cccgtggatc aaggttttct gtggctgagc atcaagagcg
     4441 ttacaaagag gaatgtcagc gcatctttga cctacagaac aaggttctgt catcaactga
     4501 agtcttatca actgacacag acagcagctc agctgaagat agtgactttg aagaaatggg
     4561 aaagaacatt gagaacatgt tgcagaacaa gaaaaccagc tctcagcttt cacgtgaacg
     4621 ggaggaacag gagcggaagg aactacagcg aatgctactg gcagcaggct cagcagcatc
     4681 cggaaacaat cacagagatg atgacacagc ttccgtgact agccttaact cttctgccac
     4741 tggacgctgt ctcaagattt atcgcacgtt tcgagatgaa gaggggaaag agtatgttcg
     4801 ctgtgagaca gtccgaaaac cagctgtcat tgatgcctat gtgcgcatac ggactacaaa
     4861 agatgaggaa ttcattcgaa aatttgccct ttttgatgaa caacatcggg aagagatgcg
     4921 aaaagaacgg cggaggattc aagagcaact gaggcggctt aagaggaacc aggaaaagga
     4981 gaagcttaag ggtcctcctg agaagaagcc caagaaaatg aaggagcgtc ctgacctaaa
     5041 actgaaatgt ggggcatgtg gtgccattgg acacatgagg actaacaaat tctgccccct
     5101 ctattatcaa acaaatgcgc caccttccaa ccctgttgcc atgacagaag aacaggagga
     5161 ggagttggaa aagacagtca ttcataatga taatgaagaa cttatcaagg ttgaagggac
     5221 caaaattgtc ttggggaaac agctaattga gagtgcggat gaggttcgca gaaaatctct
     5281 ggttctcaag tttcctaaac agcagcttcc tccaaagaag aaacggcgag ttggaaccac
     5341 tgttcactgt gactatttga atagacctca taagtccatc caccggcgcc gcacagaccc
     5401 tatggtgacg ctgtcgtcca tcttggagtc tatcatcaat gacatgagag atcttccaaa
     5461 tacataccct ttccacactc cagtcaatgc aaaggttgta aaggactact acaaaatcat
     5521 cactcggcca atggacctac aaacactccg cgaaaacgtg cgtaaacgcc tctacccatc
     5581 tcgggaagag ttcagagagc atctggagct aattgtgaaa aatagtgcaa cctacaatgg
     5641 gccaaaacac tcattgactc agatctctca atccatgctg gatctctgtg atgaaaaact
     5701 caaagagaaa gaagacaaat tagctcgctt agagaaagct atcaacccct tgctggatga
     5761 tgatgaccaa gtggcgtttt ctttcattct ggacaacatt gtcacccaga aaatgatggc
     5821 agttccagat tcttggccat ttcatcaccc agttaataag aaatttgttc cagattatta
     5881 caaagtgatt gtcaatccaa tggatttaga gaccatacgt aagaacatct ccaagcacaa
     5941 gtatcagagt cgggagagct ttctggatga tgtaaacctt attctggcca acagtgttaa
     6001 gtataatgga cctgagagtc agtatactaa gactgcccag gagattgtga acgtctgtta
     6061 ccagacattg actgagtatg atgaacattt gactcaactt gagaaggata tttgtactgc
     6121 taaagaagca gctttggagg aagcagaatt agaaagcctg gacccaatga ccccagggcc
     6181 ctacacgcct cagcctcctg atttgtatga taccaacaca tccctcagta tgtctcgaga
     6241 tgcctctgta tttcaagatg agagcaatat gtctgtcttg gatattccca gtgccactcc
     6301 agaaaagcag gtaacacagg aaggtgaaga tggagatggt gatcttgcag atgaagagga
     6361 aggaactgta caacagcctc aagccagtgt cctgtatgag gatttgctta tgtctgaagg
     6421 agaagatgat gaggaagatg ctgggagtga tgaagaagga gacaatcctt tctctgctat
     6481 ccagctgagt gaaagtggaa gtgactctga tgtgggatct ggtggaataa gacccaaaca
     6541 accccgcatg cttcaggaga acacaaggat ggacatggaa aatgaagaaa gcatgatgtc
     6601 ctatgaggga gacggtgggg aggcttccca tggtttggag gatagcaaca tcagttatgg
     6661 gagctatgag gagcctgatc ccaagtcgaa cacccaagac acaagcttca gcagcatcgg
     6721 tgggtatgag gtatcagagg aggaagaaga tgaggaggag gaagagcagc gctctgggcc
     6781 gagcgtacta agccaggtcc acctgtcaga ggacgaggag gacagtgagg atttccactc
     6841 cattgctggg gacagtgact tggactctga tgaatagtaa tgaactagtc cgtaacttga
     6901 aagtatttcg atttcttggc tttatatatc ttgtggaaag gacgacgtcg aaaccgttca
     6961 acggaaggaa cgcgttaagt cgacaatcaa cctctggatt acaaaatttg tgaaagattg
     7021 actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc tttaatgcct
     7081 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
     7141 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
     7201 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
     7261 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
     7321 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaaa
     7381 tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
     7441 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
     7501 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
     7561 gccgcctccc cgcgtcgact ttaagaccaa tgacttacaa ggcagctgta gatcttagcc
     7621 actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc
     7681 tgctttttgc ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg
     7741 gctaactagg gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag
     7801 tgtgtgcccg tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag
     7861 tgtggaaaat ctctagcagt acgtatagta gttcatgtca tcttattatt cagtatttat
     7921 aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg
     7981 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     8041 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc
     8101 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     8161 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     8221 ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt
     8281 gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     8341 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     8401 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac
     8461 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     8521 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     8581 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     8641 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     8701 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     8761 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     8821 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     8881 gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc
     8941 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     9001 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     9061 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     9121 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     9181 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     9241 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     9301 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     9361 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     9421 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     9481 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     9541 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     9601 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     9661 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     9721 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     9781 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     9841 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     9901 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     9961 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
    10021 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
    10081 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
    10141 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
    10201 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
    10261 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
    10321 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
    10381 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
    10441 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
    10501 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
    10561 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
    10621 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
    10681 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
    10741 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
    10801 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
    10861 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
    10921 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
    10981 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
    11041 aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact
    11101 aaagggaaca aaagctggag ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc
    11161 ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc
    11221 atgccgattg gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc aacagacggg
    11281 tctgacatgg attggacgaa ccactgaatt gccgcattgc agagatattg tatttaagtg
    11341 cctagctcga tacataaacg ggtctctctg gttagaccag atctgagcct gggagctctc
    11401 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
    11461 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
    11521 agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca
    11581 gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg
    11641 cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg
    11701 tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta
    11761 aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta
    11821 gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg
    11881 ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca
    11941 gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta
    12001 gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccgctgat
    12061 cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa
    12121 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca
    12181 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg
    12241 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg
    12301 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca
    12361 actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct
    12421 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc
    12481 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac
    12541 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga
    12601 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc
    12661 aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat
    12721 gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag
    12781 agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc
    12841 catgcattgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg ctccccagca
    12901 ggcagaagta tgcaaagcat gcgtctcaat tagtcagcaa ccatagtccc gcccctaact
    12961 ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta
    13021 atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt ccagaagtag
    13081 tgaggaggct tttttggagg cctaggcttt tgcaaaaagc tttctagagg taccaccgga
    13141 gcttaccatg accgagtaca agcccacggt gcgcctcgcc acccgcgacg acgtccccag
    13201 ggccgtacgc accctcgccg ccgcgttcgc cgactacccc gccacgcgcc acaccgtcga
    13261 tccggaccgc cacatcgagc gggtcaccga gctgcaagaa ctcttcctca cgcgcgtcgg
    13321 gctcgacatc ggcaaggtgt gggtcgcgga cgacggcgcc gccgtggcgg tctggaccac
    13381 gccggagagc gtcgaagcgg gggcggtgtt cgccgagatc ggcccgcgca tggccgagtt
    13441 gagcggttcc cggctggccg cgcagcaaca gatggaaggc ctcctggcgc cgcaccggcc
    13501 caaggagccc gcgtggttcc tggccaccgt cggcgtctcg cccgaccacc agggcaaggg
    13561 tctgggcagc gccgtcgtgc tccccggagt ggaggcggcc gagcgcgccg gggtgcccgc
    13621 cttcctggag acctccgcgc cccgcaacct ccccttctac gagcggctcg gcttcaccgt
    13681 caccgccgac gtcgaggtgc ccgaaggacc gcgcacctgg tgcatgaccc gcaagcccgg
    13741 tgccagttaa cgaattctag atcttgagac aaatggcagt attcatccac aattttaaaa
    13801 gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag
    13861 acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt
    13921 acagggacag cagagatcca ctttggcgcc ggctcgaggc ctgcaggtgc aaagatggat
    13981 aaagttttaa acagagagga atctttgcag ctaatggacc ttctaggtct tgaaaggagt
    14041 gggaatt
//
