LOCUS       sequence-353031-        2602 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-AC3
FEATURES             Location/Qualifiers
     source          1..2602
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(240..259)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(241..259)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(264..280)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(264..280)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      687..1275
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1176..1195
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(1402..1407,1408..1424,1425..1430)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             join(1478..2602,1..66)
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(1830..1849)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg ggccacctcg
       61 acctgaatgg aagccggcgg cacctcgcta acggattcac cactccaaga attggagcca
      121 atcaattctt gcggagaact gtgaatgcgc aaaccaaccc ttgatcgggg aagaacagta
      181 tgtcgagcta ttttttgact tactggggat caagcctgat tgggagaaaa taaaatatcc
      241 cctatagtga gtcgtattac atggtcatag ctgtttcctg gcagctctgg cccgtgtctc
      301 aaaatctctg atgttacatt gcacaagata aaaatatatc atcatgcctc ctctagacca
      361 aagctggtct cactacaact tgcgctgggg ttagtccgtg ggcttgacag agaactggta
      421 gaaggcgttg gacggtttca agagcctgtt tgccaccgtc atgactagca atcgctacaa
      481 cttgatctgg ggtgagtccg tgggcttgac agagaactgg aaggaggcgc tgaaccgtct
      541 caagagcctg tttgccacca atattactag caatcgctac cacgagacca tatctggtcg
      601 agccacccat gaccaaaatc ccttaacgtg agttacgcgt cgttccactg agcgtcagac
      661 cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc
      721 ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca
      781 actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgttcttcta
      841 gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct
      901 ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg
      961 gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc
     1021 acacagccca gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta
     1081 tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg
     1141 gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt
     1201 cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg
     1261 cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg
     1321 ccttttgctc acatgttctt tcctgcgtta tccacagaat caggggataa cgcaggaaag
     1381 aacatgaatt aattctcatg tttgacagct tatcatcgat tagctttaat gcggtagttt
     1441 atcacagtta aattgctaac gcagtcaggc accgtgtatg aaatctaaca atgcgctcat
     1501 cgtcatcctc ggcaccgtca ccctggatgc tgtaggcata ggcttggtta tgccggtact
     1561 gccgggcctc ttgcgggata tcgtccattc cgacagcatc gccagtcact atggcgtgct
     1621 gctagcgcta tatgcgttga tgcaatttct atgcgcaccc gttctcggag cactgtccga
     1681 ccgctttggc cgccgcccag tcctgctcgc ttcgctactt ggagccacta tcgactacgc
     1741 gatcatggcg accacacccg tcctgtggat tctctacgcc ggacgcatcg tggccggcat
     1801 caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc gacatcaccg atggggaaga
     1861 tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc gtgggtatgg tggcaggccc
     1921 cgtggccggg ggactgttgg gcgccatctc cttacatgca ccattccttg cggcggcggt
     1981 gctcaacggc ctcaacctac tactgggctg cttcctaatg caggagtcgc ataagggaga
     2041 gcgccgaccc atgcccttga gagccttcaa cccagtcagc tccttccggt gggcgcgggg
     2101 catgactatc gtcgccgcac ttatgactgt cttctttatc atgcaactcg taggacaggt
     2161 gccggcagcg ctctgggtca ttttcggcga ggaccgcttt cgctggagcg cgacgatgat
     2221 cggcctgtcg cttgcggtat tcggaatctt gcacgccctc gctcaagcct tcgtcactgg
     2281 tcccgccacc aaacgtttcg gcgagaagca ggccattatc gccggcatgg cggccgacgc
     2341 gctgggctac gtcttgctgg cgttcgcgac gcgaggctgg atggccttcc ccattatgat
     2401 tcttctcgct tccggcggca tcgggatgcc cgcgttgcag gccatgctgt ccaggcaggt
     2461 agatgacgac catcagggac agcttcaagg atcgctcgcg gctcttacca gcctaacttc
     2521 gatcattgga ccgctgatcg tcacggcgat ttatgccgcc tcggcgagca catggaacgg
     2581 gttggcatgg attgtaggcg cc
//
