LOCUS       sequence-353037-        2602 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2602)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2602)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-AC5
FEATURES             Location/Qualifiers
     source          1..2602
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     438..457
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(664..669,670..686,687..692)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             740..1930
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(1092..1111)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     primer_bind     complement(2104..2123)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2105..2123)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2128..2144)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2128..2144)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      join(2551..2602,1..537)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
       61 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
      121 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
      181 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
      241 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
      301 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
      361 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
      421 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
      481 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
      541 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
      601 tttcctgcgt tatccacaga atcaggggat aacgcaggaa agaacatgaa ttaattctca
      661 tgtttgacag cttatcatcg attagcttta atgcggtagt ttatcacagt taaattgcta
      721 acgcagtcag gcaccgtgta tgaaatctaa caatgcgctc atcgtcatcc tcggcaccgt
      781 caccctggat gctgtaggca taggcttggt tatgccggta ctgccgggcc tcttgcggga
      841 tatcgtccat tccgacagca tcgccagtca ctatggcgtg ctgctagcgc tatatgcgtt
      901 gatgcaattt ctatgcgcac ccgttctcgg agcactgtcc gaccgctttg gccgccgccc
      961 agtcctgctc gcttcgctac ttggagccac tatcgactac gcgatcatgg cgaccacacc
     1021 cgtcctgtgg attctctacg ccggacgcat cgtggccggc atcaccggcg ccacaggtgc
     1081 ggttgctggc gcctatatcg ccgacatcac cgatggggaa gatcgggctc gccacttcgg
     1141 gctcatgagc gcttgtttcg gcgtgggtat ggtggcaggc cccgtggccg ggggactgtt
     1201 gggcgccatc tccttacatg caccattcct tgcggcggcg gtgctcaacg gcctcaacct
     1261 actactgggc tgcttcctaa tgcaggagtc gcataaggga gagcgccgac ccatgccctt
     1321 gagagccttc aacccagtca gctccttccg gtgggcgcgg ggcatgacta tcgtcgccgc
     1381 acttatgact gtcttcttta tcatgcaact cgtaggacag gtgccggcag cgctctgggt
     1441 cattttcggc gaggaccgct ttcgctggag cgcgacgatg atcggcctgt cgcttgcggt
     1501 attcggaatc ttgcacgccc tcgctcaagc cttcgtcact ggtcccgcca ccaaacgttt
     1561 cggcgagaag caggccatta tcgccggcat ggcggccgac gcgctgggct acgtcttgct
     1621 ggcgttcgcg acgcgaggct ggatggcctt ccccattatg attcttctcg cttccggcgg
     1681 catcgggatg cccgcgttgc aggccatgct gtccaggcag gtagatgacg accatcaggg
     1741 acagcttcaa ggatcgctcg cggctcttac cagcctaact tcgatcattg gaccgctgat
     1801 cgtcacggcg atttatgccg cctcggcgag cacatggaac gggttggcat ggattgtagg
     1861 cgccgcccta taccttgtct gcctccccgc gttgcgtcgc ggtgcatgga gccgggccac
     1921 ctcgacctga atggaagccg gcggcacctc gctaacggat tcaccactcc aagaattgga
     1981 gccaatcaat tcttgcggag aactgtgaat gcgcaaacca acccttgatc ggggaagaac
     2041 agtatgtcga gctatttttt gacttactgg ggatcaagcc tgattgggag aaaataaaat
     2101 atcccctata gtgagtcgta ttacatggtc atagctgttt cctggcagct ctggcccgtg
     2161 tctcaaaatc tctgatgtta cattgcacaa gataaaaata tatcatcatg cctcctctag
     2221 accaaagctg gtctcggcaa ttgctagtaa tattggtggc aaacaggctc ttgagacggt
     2281 tcagcgcctc cttccagttc tctgtcaagc ccacggactc accccagatc aagttgtagc
     2341 gattgctagt catgacggtg gcaaacaggc tcttgaaacc gtccaacgcc ttctaccagt
     2401 tctctgtcaa gcccacggac taaccccagc gcaagttgta gcgatttgag accatatctg
     2461 gtcgagccac ccatgaccaa aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc
     2521 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     2581 ctgcttgcaa acaaaaaaac ca
//
