LOCUS       sequence-353045-        2600 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-AC9
FEATURES             Location/Qualifiers
     source          1..2600
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(332..351)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(333..351)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(356..372)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(356..372)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     rep_origin      777..1365
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1266..1285
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        join(1492..1497,1498..1514,1515..1520)
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     CDS             join(1568..2600,1..158)
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     complement(1920..1939)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gcctaacttc gatcattgga ccgctgatcg tcacggcgat ttatgccgcc tcggcgagca
       61 catggaacgg gttggcatgg attgtaggcg ccgccctata ccttgtctgc ctccccgcgt
      121 tgcgtcgcgg tgcatggagc cgggccacct cgacctgaat ggaagccggc ggcacctcgc
      181 taacggattc accactccaa gaattggagc caatcaattc ttgcggagaa ctgtgaatgc
      241 gcaaaccaac ccttgatcgg ggaagaacag tatgtcgagc tattttttga cttactgggg
      301 atcaagcctg attgggagaa aataaaatat cccctatagt gagtcgtatt acatggtcat
      361 agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga
      421 taaaaatata tcatcatgcc tcctctagac caaagctggt ctcgttccaa tattggtggc
      481 aaacaggctc ttgagacggt tcagcgcctc cttccagttc tctgtcaagc ccacggactc
      541 accccagatc aagttgtagc gattgctagt catgacggtg gcaaacaggc tcttgaaacc
      601 gtccaacgcc ttctaccagt tctctgtcaa gcccacggac taaccccagc gcaagttgta
      661 gcgattgcct cttgagacca tatctggtcg agccacccat gaccaaaatc ccttaacgtg
      721 agttacgcgt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
      781 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
      841 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
      901 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
      961 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     1021 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     1081 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     1141 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     1201 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     1261 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     1321 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     1381 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     1441 tccacagaat caggggataa cgcaggaaag aacatgaatt aattctcatg tttgacagct
     1501 tatcatcgat tagctttaat gcggtagttt atcacagtta aattgctaac gcagtcaggc
     1561 accgtgtatg aaatctaaca atgcgctcat cgtcatcctc ggcaccgtca ccctggatgc
     1621 tgtaggcata ggcttggtta tgccggtact gccgggcctc ttgcgggata tcgtccattc
     1681 cgacagcatc gccagtcact atggcgtgct gctagcgcta tatgcgttga tgcaatttct
     1741 atgcgcaccc gttctcggag cactgtccga ccgctttggc cgccgcccag tcctgctcgc
     1801 ttcgctactt ggagccacta tcgactacgc gatcatggcg accacacccg tcctgtggat
     1861 tctctacgcc ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc
     1921 ctatatcgcc gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc
     1981 ttgtttcggc gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc
     2041 cttacatgca ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg
     2101 cttcctaatg caggagtcgc ataagggaga gcgccgaccc atgcccttga gagccttcaa
     2161 cccagtcagc tccttccggt gggcgcgggg catgactatc gtcgccgcac ttatgactgt
     2221 cttctttatc atgcaactcg taggacaggt gccggcagcg ctctgggtca ttttcggcga
     2281 ggaccgcttt cgctggagcg cgacgatgat cggcctgtcg cttgcggtat tcggaatctt
     2341 gcacgccctc gctcaagcct tcgtcactgg tcccgccacc aaacgtttcg gcgagaagca
     2401 ggccattatc gccggcatgg cggccgacgc gctgggctac gtcttgctgg cgttcgcgac
     2461 gcgaggctgg atggccttcc ccattatgat tcttctcgct tccggcggca tcgggatgcc
     2521 cgcgttgcag gccatgctgt ccaggcaggt agatgacgac catcagggac agcttcaagg
     2581 atcgctcgcg gctcttacca
//
