LOCUS       sequence-353047-       10360 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10360)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 10360)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF0269
FEATURES             Location/Qualifiers
     source          1..10360
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    3298..3886
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3351..3371)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3769..3780)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3958..4191
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4269..4390
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4306..4325)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4360..4379
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4430..4565
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4585..4604)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4586..4604)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4614..4631)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4614..4630)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4623..4645)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4772..5227
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4859..4878)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5069..5090
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5253..5357
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5358..5426,5427..6218)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5576..5595)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6389..6977
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6878..6897
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7131..7148
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7265..7286
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7301..7306,7307..7324,7325..7331)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7339..7355
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7344..7366
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7363..7379
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7363..7379
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7398..7418
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7400..7418
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7446..7672
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7673..7853
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7900..8025
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8518..8751
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8936..8980
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        9211..9421
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        9233..9429
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      9280..9415
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9461..10063
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(9461..9480)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9957..9977
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    10106..10223
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgca
     1201 acgggcaaaa actgaactgt tcaggcttca gattgtaact gacgatctga ggaaaaatga
     1261 gatgttttca cctcctgtga gcagtgggaa aaatggacca acttctttgg caagtggaca
     1321 ttttactggc tcaaatgtag aagacagaag tagctcaggg tcctggggga atggaggaca
     1381 tccaagcccg tccaggaact atggagatgg gactccctat gaccacatga ccagcaggga
     1441 ccttgggtca catgacaatc tctctccacc ttttgtcaat tccagaatac aaagtaaaac
     1501 agaaaggggc tcatactcat cttatgggag agaatcaaac ttacagggtt gccaccagca
     1561 gagtctcctt ggaggtgaca tggatatggg caacccagga accctttcgc ccaccaaacc
     1621 tggttcccag tactatcagt attctagcaa taatccccga aggaggcctc ttcacagtag
     1681 tgccatggag gtacagacaa agaaagttcg aaaagttcct ccaggtttgc catcttcagt
     1741 ctatgctcca tcagcaagca ctgccgacta caatagggac tcgccaggct atccttcctc
     1801 caaaccagca accagcactt tccctagctc cttcttcatg caagatggcc atcacagcag
     1861 tgacccttgg agctcctcca gtgggatgaa tcagcctggc tatgcaggaa tgttgggcaa
     1921 ctcttctcat attccacagt ccagcagcta ctgtagcctg catccacatg aacgtttgag
     1981 ctatccatca cactcctcag cagacatcaa ttccagtctt cctccgatgt ccactttcca
     2041 tcgtagtggt acaaaccatt acagcacctc ttcctgtacg cctcctgcca acgggacaga
     2101 cagtataatg gcaaatagag gaagcggggc agccggcagc tcccagactg gagatgctct
     2161 ggggaaagca cttgcttcga tctattctcc agatcacact aacaacagct tttcatcaaa
     2221 cccttcaact cctgttggct ctcctccatc tctctcaggc acagctgttt ggtctagaaa
     2281 tggaggacag gcctcatcgt ctcctaatta tgaaggaccc ttacactctt tgcaaagccg
     2341 aattgaagat cgtttagaaa gactggatga tgctattcat gttctccgga accatgcagt
     2401 gggcccatcc acagctatgc ctggtggtca tggggacatg catggaatca ttggaccttc
     2461 tcataatgga gccatgggtg gtctgggctc agggtatgga accggccttc tttcagccaa
     2521 cagacattca ctcatggtgg ggacccatcg tgaagatggc gtggccctga gaggcagcca
     2581 ttctcttctg ccaaaccagg ttccggttcc acagcttcct gtccagtctg cgacttcccc
     2641 tgacctgaac ccaccccagg acccttacag aggcatgcca ccaggactac aggggcagag
     2701 tgtctcctct ggcagctctg agatcaaatc cgatgacgag ggtgatgaga acctgcaaga
     2761 cacgaaatct tcggaggaca agaaattaga tgacgacaag aaggatatca aatcaattac
     2821 tagcaataat gacgatgagg acctgacacc agagcagaag gcagagcgtg agaaggagcg
     2881 gaggatggcc aacaatgccc gagagcgtct gcgggtccgt gacatcaacg aggctttcaa
     2941 agagctcggc cgcatggtgc agctccacct caagagtgac aagccccaga ccaagctcct
     3001 gatcctccac caggcggtgg ccgtcatcct cagtctggag cagcaagtcc gagaaaggaa
     3061 tctgaatccg aaagctgcgt gtctgaaaag aagggaggaa gagaaggtgt cctcagagcc
     3121 tccccctctc tccttggccg gcccacaccc tggaatggga gacgcatcga atcacatggg
     3181 acagatgtag taatgaacta gtccgtaact tgaaagtatt tcgatttctt ggctttatat
     3241 atcttgtgga aaggacgagt tacagtcgtc gaagttcaaa gcacgcgtta agtcgacaat
     3301 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     3361 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     3421 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     3481 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     3541 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     3601 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     3661 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     3721 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     3781 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     3841 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcgtcg actttaagac
     3901 caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg
     3961 aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact gggtctctct
     4021 ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc
     4081 ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg
     4141 gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc agtacgtata
     4201 gtagttcatg tcatcttatt attcagtatt tataacttgc aaagaaatga atatcagaga
     4261 gtgagaggaa cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa
     4321 atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca
     4381 atgtatctta tcatgtctgg ctctagctat cccgccccta actccgccca tcccgcccct
     4441 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     4501 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
     4561 aggcctaggg acgtacccaa ttcgccctat agtgagtcgt attacgcgcg ctcactggcc
     4621 gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca
     4681 gcacatcccc ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
     4741 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
     4801 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
     4861 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
     4921 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
     4981 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
     5041 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
     5101 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
     5161 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
     5221 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
     5281 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
     5341 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
     5401 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
     5461 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
     5521 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
     5581 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
     5641 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
     5701 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
     5761 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
     5821 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
     5881 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
     5941 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
     6001 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
     6061 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
     6121 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
     6181 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
     6241 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
     6301 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
     6361 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
     6421 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
     6481 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
     6541 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
     6601 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
     6661 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
     6721 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
     6781 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
     6841 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
     6901 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
     6961 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
     7021 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
     7081 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
     7141 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
     7201 ttcattaatg cagctggcac gacaggtttc ccgactggaa agcgggcagt gagcgcaacg
     7261 caattaatgt gagttagctc actcattagg caccccaggc tttacacttt atgcttccgg
     7321 ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca cacaggaaac agctatgacc
     7381 atgattacgc caagcgcgca attaaccctc actaaaggga acaaaagctg gagctgcaag
     7441 cttaatgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
     7501 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
     7561 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
     7621 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
     7681 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     7741 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     7801 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
     7861 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
     7921 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
     7981 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
     8041 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
     8101 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
     8161 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
     8221 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
     8281 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
     8341 agtaagacca ccgcacagca agcggccgct gatcttcaga cctggaggag gagatatgag
     8401 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     8461 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     8521 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     8581 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     8641 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     8701 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     8761 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     8821 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     8881 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     8941 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     9001 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     9061 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     9121 gtttcagacc cacctcccaa ccccgagggg acccatgcat tgcatctcaa ttagtcagca
     9181 accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcgtctc
     9241 aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     9301 agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     9361 gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     9421 ttttgcaaaa agctttctag aggtaccacc ggagcttacc atgaccgagt acaagcccac
     9481 ggtgcgcctc gccacccgcg acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt
     9541 cgccgactac cccgccacgc gccacaccgt cgatccggac cgccacatcg agcgggtcac
     9601 cgagctgcaa gaactcttcc tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc
     9661 ggacgacggc gccgccgtgg cggtctggac cacgccggag agcgtcgaag cgggggcggt
     9721 gttcgccgag atcggcccgc gcatggccga gttgagcggt tcccggctgg ccgcgcagca
     9781 acagatggaa ggcctcctgg cgccgcaccg gcccaaggag cccgcgtggt tcctggccac
     9841 cgtcggcgtc tcgcccgacc accagggcaa gggtctgggc agcgccgtcg tgctccccgg
     9901 agtggaggcg gccgagcgcg ccggggtgcc cgccttcctg gagacctccg cgccccgcaa
     9961 cctccccttc tacgagcggc tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg
    10021 accgcgcacc tggtgcatga cccgcaagcc cggtgccagt taacgaattc tagatcttga
    10081 gacaaatggc agtattcatc cacaatttta aaagaaaagg ggggattggg gggtacagtg
    10141 caggggaaag aatagtagac ataatagcaa cagacataca aactaaagaa ttacaaaaac
    10201 aaattacaaa aattcaaaat tttcgggttt attacaggga cagcagagat ccactttggc
    10261 gccggctcga ggcctgcagg tgcaaagatg gataaagttt taaacagaga ggaatctttg
    10321 cagctaatgg accttctagg tcttgaaagg agtgggaatt
//
