LOCUS       sequence-353126-        8802 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables spatial detection of 
            CRISPR screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8802)
  AUTHORS   Dhainaut M, Rose SA, Akturk G, Wroblewska A, Nielsen SR, Park ES, 
            Buckup M, Roudko V, Pia L, Sweeney R, Le Berichel J, Wilk CM, 
            Bektesevic A, Lee BH, Bhardwaj N, Rahman AH, Baccarini A, Gnjatic S,
            Pe'er D, Merad M, Brown BD
  TITLE     Spatial CRISPR genomics identifies regulators of the tumor 
            microenvironment.
  JOURNAL   Cell. 2022 Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015.
  PUBMED    35290801
REFERENCE   2  (bases 1 to 8802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2022 
            Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015."
COMMENT     Sequence Label: pLKO_HA.Ollas.S_mCherry-NLS
FEATURES             Location/Qualifiers
     source          1..8802
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        97..201
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(202..270,271..1062)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(420..439)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1233..1821
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1722..1741
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1975..1992
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2109..2130
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2145..2150,2151..2168,2169..2175)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2183..2199
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2188..2210
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2207..2223
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2207..2223
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2242..2262
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2244..2262
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2290..2516
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2517..2697
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2744..2869
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3362..3595
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3780..3824
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        4122..4370
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     4122..4142
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     4293..4312
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        4389..4464
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    4512..4629
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4769..5947
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5000..5938
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5895..5915
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      5996..6005
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6008..6034
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6053..6094
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             6116..6160
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             6170..6877
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6315..6333)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6583..6602
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    6947..7535
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7000..7020)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7418..7429)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7610..7843
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7915..8036
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7952..7971)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8006..8025
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8076..8211
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8231..8250)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8232..8250)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8260..8277)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8260..8276)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8269..8291)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      join(8418..8802,1..71)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8505..8524)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8715..8736
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
       61 gcttacaatt taggtggcac ttttcgggga aatgtgcgcg gaacccctat ttgtttattt
      121 ttctaaatac attcaaatat gtatccgctc atgagacaat aaccctgata aatgcttcaa
      181 taatattgaa aaaggaagag tatgagtatt caacatttcc gtgtcgccct tattcccttt
      241 tttgcggcat tttgccttcc tgtttttgct cacccagaaa cgctggtgaa agtaaaagat
      301 gctgaagatc agttgggtgc acgagtgggt tacatcgaac tggatctcaa cagcggtaag
      361 atccttgaga gttttcgccc cgaagaacgt tttccaatga tgagcacttt taaagttctg
      421 ctatgtggcg cggtattatc ccgtattgac gccgggcaag agcaactcgg tcgccgcata
      481 cactattctc agaatgactt ggttgagtac tcaccagtca cagaaaagca tcttacggat
      541 ggcatgacag taagagaatt atgcagtgct gccataacca tgagtgataa cactgcggcc
      601 aacttacttc tgacaacgat cggaggaccg aaggagctaa ccgctttttt gcacaacatg
      661 ggggatcatg taactcgcct tgatcgttgg gaaccggagc tgaatgaagc cataccaaac
      721 gacgagcgtg acaccacgat gcctgtagca atggcaacaa cgttgcgcaa actattaact
      781 ggcgaactac ttactctagc ttcccggcaa caattaatag actggatgga ggcggataaa
      841 gttgcaggac cacttctgcg ctcggccctt ccggctggct ggtttattgc tgataaatct
      901 ggagccggtg agcgtgggtc tcgcggtatc attgcagcac tggggccaga tggtaagccc
      961 tcccgtatcg tagttatcta cacgacgggg agtcaggcaa ctatggatga acgaaataga
     1021 cagatcgctg agataggtgc ctcactgatt aagcattggt aactgtcaga ccaagtttac
     1081 tcatatatac tttagattga tttaaaactt catttttaat ttaaaaggat ctaggtgaag
     1141 atcctttttg ataatctcat gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg
     1201 tcagaccccg tagaaaagat caaaggatct tcttgagatc ctttttttct gcgcgtaatc
     1261 tgctgcttgc aaacaaaaaa accaccgcta ccagcggtgg tttgtttgcc ggatcaagag
     1321 ctaccaactc tttttccgaa ggtaactggc ttcagcagag cgcagatacc aaatactgtt
     1381 cttctagtgt agccgtagtt aggccaccac ttcaagaact ctgtagcacc gcctacatac
     1441 ctcgctctgc taatcctgtt accagtggct gctgccagtg gcgataagtc gtgtcttacc
     1501 gggttggact caagacgata gttaccggat aaggcgcagc ggtcgggctg aacggggggt
     1561 tcgtgcacac agcccagctt ggagcgaacg acctacaccg aactgagata cctacagcgt
     1621 gagctatgag aaagcgccac gcttcccgaa gggagaaagg cggacaggta tccggtaagc
     1681 ggcagggtcg gaacaggaga gcgcacgagg gagcttccag ggggaaacgc ctggtatctt
     1741 tatagtcctg tcgggtttcg ccacctctga cttgagcgtc gatttttgtg atgctcgtca
     1801 ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt cctggccttt
     1861 tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt ggataaccgt
     1921 attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga gcgcagcgag
     1981 tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc cgcgcgttgg
     2041 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     2101 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
     2161 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg aaacagctat
     2221 gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa gctggagctg
     2281 caagcttaat gtagtcttat gcaatactct tgtagtcttg caacatggta acgatgagtt
     2341 agcaacatgc cttacaagga gagaaaaagc accgtgcatg gcgattggtg gaagtaaggt
     2401 ggtacgatcg tgccttatta ggaaggcaac agacgggtct gacatggatt ggacgaacca
     2461 ctgaattgcc gcattgcaga gatattgtat ttaagtgcct agctcgatac ataaacgggt
     2521 ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg aacccactgc
     2581 ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg
     2641 actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc tctagcagtg
     2701 gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc gacgcaggac
     2761 tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga gtacgccaaa
     2821 aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca gtattaagcg
     2881 ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa agaaaaaata
     2941 taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag ttaatcctgg
     3001 cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac catcccttca
     3061 gacaggatca gaagaactta gatcattata taatacagta gcaaccctct attgtgtgca
     3121 tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg aagagcaaaa
     3181 caaaagtaag accaccgcac agcaagcggc cgctgatctt cagacctgga ggaggagata
     3241 tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag
     3301 gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa
     3361 taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa
     3421 tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt
     3481 tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc
     3541 agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga
     3601 tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga
     3661 gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa
     3721 ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa
     3781 agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca
     3841 taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt
     3901 taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat
     3961 tatcgtttca gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag
     4021 aagaaggtgg agagagagac agagacagat ccattcgatt agtgaacgga tctcgacggt
     4081 atcgatcacg agactagtat cgagcggccg cccccttcac cgagggccta tttcccatga
     4141 ttccttcata tttgcatata cgatacaagg ctgttagaga gataattgga attaatttga
     4201 ctgtaaacac aaagatatta gtacaaaata cgtgacgtag aaagtaataa tttcttgggt
     4261 agtttgcagt tttaaaatta tgttttaaaa tggactatca tatgcttacc gtaacttgaa
     4321 agtatttcga tttcttggct ttatatatct tgtggaaagg acgaaacacc gggtcttcga
     4381 gaagacctgt tttagagcta gaaatagcaa gttaaaataa ggctagtccg ttatcaactt
     4441 gaaaaagtgg caccgagtcg gtgctttttt taattctcga cctcgagaca aatggcagta
     4501 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
     4561 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
     4621 caaaattttc gggtttatta cagggacagc agagatccac tttggccgcg gctcgaggcg
     4681 ataagctttg caaagatgga taaagtttta aacagagagg aatctttgca gctaatggac
     4741 cttctaggtc ttgaaaggag tgggaattgg ctccggtgcc cgtcagtggg cagagcgcac
     4801 atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaaccg gtgcctagag
     4861 aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc tttttcccga
     4921 gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt ttcgcaacgg
     4981 gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg gcctctttac
     5041 gggttatggc ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg tgattcttga
     5101 tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt aaggagcccc
     5161 ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg tgcgaatctg
     5221 gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt aaaatttttg
     5281 atgacctgct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg gccaagatct
     5341 gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg cgtcccagcg
     5401 cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac gggggtagtc
     5461 tcaagctggc cggcctgctc tggtgcctgg cctcgcgccg ccgtgtatcg ccccgccctg
     5521 ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc cgcttcccgg
     5581 ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg cgggtgagtc
     5641 acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg actccacgga
     5701 gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta cgtcgtcttt
     5761 aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg tggagactga
     5821 agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt ttgagtttgg
     5881 atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc catttcaggt
     5941 gtcgtgagga attctgcagt cgatcgacgg taccgcgggc ccgggatcca cggtcgccac
     6001 catgggctac ccatacgatg tgccagatta cgctggagga ggcgggggag ggagcggctt
     6061 cgccaacgag ctgggcccca ggctgatggg caagtgcgga ggaggcgggg gagggaagga
     6121 gaccgccgcc gccaagttcg agaggcagca catggacagc ggcatgctga tggtgagcaa
     6181 gggcgaggag gataacatgg ccatcatcaa ggagttcatg cgcttcaagg tgcacatgga
     6241 gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc gcccctacga
     6301 gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct tcgcctggga
     6361 catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc ccgccgacat
     6421 ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg tgatgaactt
     6481 cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg gcgagttcat
     6541 ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa tgcagaagaa
     6601 gaccatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggcg ccctgaaggg
     6661 cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgctg aggtcaagac
     6721 cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca acatcaagtt
     6781 ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac gcgccgaggg
     6841 ccgccactcc accggcggca tggacgagct gtacaagaaa cggccagccg aggatatgga
     6901 ggaagaacaa gcctttaaac gatctcgata aagcggccgc gtcgacaatc aacctctgga
     6961 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
     7021 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
     7081 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
     7141 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
     7201 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
     7261 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
     7321 ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct gtgttgccac
     7381 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
     7441 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg catcttcgcc ttcgccctca
     7501 gacgagtcgg atctcccttt gggccgcctc cccgcctggg tacctttaag accaatgact
     7561 tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta
     7621 attcactccc aacgaagata agatctgctt tttgcttgta ctgggtctct ctggttagac
     7681 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
     7741 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
     7801 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat
     7861 cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg
     7921 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     7981 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     8041 gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     8101 ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     8161 cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt
     8221 acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg
     8281 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
     8341 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
     8401 cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     8461 tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     8521 cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     8581 tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     8641 tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     8701 cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     8761 ctattctttt gatttataag ggattttgcc gatttcggcc ta
//
