LOCUS       sequence-353128-        8799 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables spatial detection of 
            CRISPR screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8799)
  AUTHORS   Dhainaut M, Rose SA, Akturk G, Wroblewska A, Nielsen SR, Park ES, 
            Buckup M, Roudko V, Pia L, Sweeney R, Le Berichel J, Wilk CM, 
            Bektesevic A, Lee BH, Bhardwaj N, Rahman AH, Baccarini A, Gnjatic S,
            Pe'er D, Merad M, Brown BD
  TITLE     Spatial CRISPR genomics identifies regulators of the tumor 
            microenvironment.
  JOURNAL   Cell. 2022 Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015.
  PUBMED    35290801
REFERENCE   2  (bases 1 to 8799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2022 
            Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015."
COMMENT     Sequence Label: pLKO_HA.Ollas.V5_mCherry-NLS
FEATURES             Location/Qualifiers
     source          1..8799
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        170..274
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(275..343,344..1135)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(493..512)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1306..1894
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1795..1814
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2048..2065
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2182..2203
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2218..2223,2224..2241,2242..2248)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2256..2272
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2261..2283
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2280..2296
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2280..2296
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2315..2335
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2317..2335
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2363..2589
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2590..2770
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2817..2942
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3435..3668
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3853..3897
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        4195..4443
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     4195..4215
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     4366..4385
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        4462..4537
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    4585..4702
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4842..6020
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5073..6011
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5968..5988
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      6069..6078
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6081..6107
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6126..6167
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             6189..6230
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6240..6947
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6385..6403)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6653..6672
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    7017..7605
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7070..7090)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7488..7499)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7680..7913
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7985..8106
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8022..8041)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8076..8095
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8146..8281
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8301..8320)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8302..8320)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8330..8347)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8330..8346)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8339..8361)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      join(8488..8799,1..144)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8575..8594)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     join(8785..8799,1..7)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
       61 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaatttta
      121 acaaaatatt aacgcttaca atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc
      181 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
      241 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
      301 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
      361 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
      421 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
      481 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc aagagcaact
      541 cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
      601 gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
      661 taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
      721 tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
      781 agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa caacgttgcg
      841 caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
      901 ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
      961 tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     1021 agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     1081 tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     1141 agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     1201 gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     1261 gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     1321 tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     1381 gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     1441 accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     1501 accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     1561 gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     1621 ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     1681 atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     1741 gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     1801 cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     1861 gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     1921 gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     1981 tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     2041 cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct
     2101 ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc
     2161 gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt
     2221 acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac
     2281 aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact aaagggaaca
     2341 aaagctggag ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg
     2401 gtaacgatga gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc atggcgattg
     2461 gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc aacagacggg tctgacatgg
     2521 attggacgaa ccactgaatt gccgcattgc agagatattg tatttaagtg cctagctcga
     2581 tacataaacg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     2641 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     2701 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     2761 atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca gaggagctct
     2821 ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg cggcgactgg
     2881 tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg tgcgagagcg
     2941 tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta aggccagggg
     3001 gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta gaacgattcg
     3061 cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg ggacagctac
     3121 aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca gtagcaaccc
     3181 tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta gacaagatag
     3241 aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccgctgat cttcagacct
     3301 ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa agtagtaaaa
     3361 attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca gagagaaaaa
     3421 agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg aagcactatg
     3481 ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg tatagtgcag
     3541 cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca actcacagtc
     3601 tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa
     3661 cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc tgtgccttgg
     3721 aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac ctggatggag
     3781 tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga agaatcgcaa
     3841 aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc aagtttgtgg
     3901 aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat gatagtagga
     3961 ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag agttaggcag
     4021 ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc cgacaggccc
     4081 gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg attagtgaac
     4141 ggatctcgac ggtatcgatc acgagactag tatcgagcgg ccgccccctt caccgagggc
     4201 ctatttccca tgattccttc atatttgcat atacgataca aggctgttag agagataatt
     4261 ggaattaatt tgactgtaaa cacaaagata ttagtacaaa atacgtgacg tagaaagtaa
     4321 taatttcttg ggtagtttgc agttttaaaa ttatgtttta aaatggacta tcatatgctt
     4381 accgtaactt gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgaaac
     4441 accgggtctt cgagaagacc tgttttagag ctagaaatag caagttaaaa taaggctagt
     4501 ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt ttttaattct cgacctcgag
     4561 acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg ggtacagtgc
     4621 aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca
     4681 aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc cactttggcc
     4741 gcggctcgag gcgataagct ttgcaaagat ggataaagtt ttaaacagag aggaatcttt
     4801 gcagctaatg gaccttctag gtcttgaaag gagtgggaat tggctccggt gcccgtcagt
     4861 gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc ggcaattgaa
     4921 ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg tactggctcc
     4981 gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc
     5041 tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc
     5101 ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc tggctgcagt
     5161 acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg aggccttgcg
     5221 cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc
     5281 gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag tctctagcca
     5341 tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt cttgtaaatg
     5401 cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc gacggggccc
     5461 gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg
     5521 gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta
     5581 tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga gcggaaagat
     5641 ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc tcgggagagc
     5701 gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat
     5761 gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg agcttttgga
     5821 gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc cacactgagt
     5881 gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg gaatttgccc
     5941 tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa agtttttttc
     6001 ttccatttca ggtgtcgtga ggaattctgc agtcgatcga cggtaccgcg ggcccgggat
     6061 ccacggtcgc caccatgggc tacccatacg atgtgccaga ttacgctgga ggaggcgggg
     6121 gagggagcgg cttcgccaac gagctgggcc ccaggctgat gggcaagtgc ggaggaggcg
     6181 ggggaggggg caaaccaatt ccaaacccac tgctgggact ggattccaca ggcatgctga
     6241 tggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg cgcttcaagg
     6301 tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag ggcgagggcc
     6361 gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc cccctgccct
     6421 tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac gtgaagcacc
     6481 ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag tgggagcgcg
     6541 tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc ctgcaggacg
     6601 gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac ggccccgtaa
     6661 tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc gaggacggcg
     6721 ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac tacgacgctg
     6781 aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc tacaacgtca
     6841 acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa cagtacgaac
     6901 gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagaaa cggccagccg
     6961 aggatatgga ggaagaacaa gcctttaaac gatctcgata aagcggccgc gtcgacaatc
     7021 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
     7081 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
     7141 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
     7201 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
     7261 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
     7321 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
     7381 gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct
     7441 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
     7501 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg catcttcgcc
     7561 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctggg tacctttaag
     7621 accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa aggggggact
     7681 ggaagggcta attcactccc aacgaagata agatctgctt tttgcttgta ctgggtctct
     7741 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
     7801 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
     7861 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtagtag
     7921 ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat cagagagtga
     7981 gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     8041 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     8101 atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc gcccctaact
     8161 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     8221 gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     8281 ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg
     8341 ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac
     8401 atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac
     8461 agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta agcgcggcgg
     8521 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     8581 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     8641 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     8701 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     8761 cgttggagtc cacgttcttt aatagtggac tcttgttcc
//
