LOCUS       sequence-353129-        8790 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables spatial detection of 
            CRISPR screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8790)
  AUTHORS   Dhainaut M, Rose SA, Akturk G, Wroblewska A, Nielsen SR, Park ES, 
            Buckup M, Roudko V, Pia L, Sweeney R, Le Berichel J, Wilk CM, 
            Bektesevic A, Lee BH, Bhardwaj N, Rahman AH, Baccarini A, Gnjatic S,
            Pe'er D, Merad M, Brown BD
  TITLE     Spatial CRISPR genomics identifies regulators of the tumor 
            microenvironment.
  JOURNAL   Cell. 2022 Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015.
  PUBMED    35290801
REFERENCE   2  (bases 1 to 8790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2022 
            Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015."
COMMENT     Sequence Label: pLKO_HA.S.VSVg_mCherry-NLS
FEATURES             Location/Qualifiers
     source          1..8790
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        150..254
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(255..323,324..1115)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(473..492)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1286..1874
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1775..1794
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     2028..2045
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2162..2183
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2198..2203,2204..2221,2222..2228)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2236..2252
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2241..2263
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2260..2276
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2260..2276
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2295..2315
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2297..2315
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2343..2569
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2570..2750
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2797..2922
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3415..3648
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3833..3877
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        4175..4423
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     4175..4195
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     4346..4365
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        4442..4517
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    4565..4682
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4822..6000
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          5053..5991
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5948..5968
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      6049..6058
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6061..6087
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6106..6150
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             6169..6201
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
     CDS             6211..6918
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6356..6374)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6624..6643
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    6988..7576
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7041..7061)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7459..7470)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7651..7884
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7956..8077
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7993..8012)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8047..8066
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8117..8252
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8272..8291)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8273..8291)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8301..8318)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8301..8317)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8310..8332)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      join(8459..8790,1..124)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8546..8565)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8756..8777
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 cctatctcgg tctattcttt tgatttataa gggattttgc cgatttcggc ctattggtta
       61 aaaaatgagc tgatttaaca aaaatttaac gcgaatttta acaaaatatt aacgcttaca
      121 atttaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
      181 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
      241 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
      301 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
      361 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
      421 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
      481 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
      541 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
      601 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
      661 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
      721 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
      781 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
      841 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
      901 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
      961 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     1021 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     1081 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     1141 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     1201 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     1261 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     1321 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     1381 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     1441 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     1501 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     1561 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     1621 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     1681 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     1741 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     1801 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     1861 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     1921 cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     1981 cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     2041 gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc
     2101 attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag cgcaacgcaa
     2161 ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg cttccggctc
     2221 gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc tatgaccatg
     2281 attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctgcaagctt
     2341 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
     2401 tgccttacaa ggagagaaaa agcaccgtgc atggcgattg gtggaagtaa ggtggtacga
     2461 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
     2521 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
     2581 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     2641 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     2701 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
     2761 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
     2821 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
     2881 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
     2941 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
     3001 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
     3061 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
     3121 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
     3181 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
     3241 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
     3301 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     3361 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     3421 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     3481 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     3541 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     3601 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     3661 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     3721 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     3781 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     3841 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     3901 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     3961 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     4021 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     4081 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgatc
     4141 acgagactag tatcgagcgg ccgccccctt caccgagggc ctatttccca tgattccttc
     4201 atatttgcat atacgataca aggctgttag agagataatt ggaattaatt tgactgtaaa
     4261 cacaaagata ttagtacaaa atacgtgacg tagaaagtaa taatttcttg ggtagtttgc
     4321 agttttaaaa ttatgtttta aaatggacta tcatatgctt accgtaactt gaaagtattt
     4381 cgatttcttg gctttatata tcttgtggaa aggacgaaac accgggtctt cgagaagacc
     4441 tgttttagag ctagaaatag caagttaaaa taaggctagt ccgttatcaa cttgaaaaag
     4501 tggcaccgag tcggtgcttt ttttaattct cgacctcgag acaaatggca gtattcatcc
     4561 acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
     4621 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt
     4681 ttcgggttta ttacagggac agcagagatc cactttggcc gcggctcgag gcgataagct
     4741 ttgcaaagat ggataaagtt ttaaacagag aggaatcttt gcagctaatg gaccttctag
     4801 gtcttgaaag gagtgggaat tggctccggt gcccgtcagt gggcagagcg cacatcgccc
     4861 acagtccccg agaagttggg gggaggggtc ggcaattgaa ccggtgccta gagaaggtgg
     4921 cgcggggtaa actgggaaag tgatgtcgtg tactggctcc gcctttttcc cgagggtggg
     4981 ggagaaccgt atataagtgc agtagtcgcc gtgaacgttc tttttcgcaa cgggtttgcc
     5041 gccagaacac aggtaagtgc cgtgtgtggt tcccgcgggc ctggcctctt tacgggttat
     5101 ggcccttgcg tgccttgaat tacttccacc tggctgcagt acgtgattct tgatcccgag
     5161 cttcgggttg gaagtgggtg ggagagttcg aggccttgcg cttaaggagc cccttcgcct
     5221 cgtgcttgag ttgaggcctg gcctgggcgc tggggccgcc gcgtgcgaat ctggtggcac
     5281 cttcgcgcct gtctcgctgc tttcgataag tctctagcca tttaaaattt ttgatgacct
     5341 gctgcgacgc tttttttctg gcaagatagt cttgtaaatg cgggccaaga tctgcacact
     5401 ggtatttcgg tttttggggc cgcgggcggc gacggggccc gtgcgtccca gcgcacatgt
     5461 tcggcgaggc ggggcctgcg agcgcggcca ccgagaatcg gacgggggta gtctcaagct
     5521 ggccggcctg ctctggtgcc tggcctcgcg ccgccgtgta tcgccccgcc ctgggcggca
     5581 aggctggccc ggtcggcacc agttgcgtga gcggaaagat ggccgcttcc cggccctgct
     5641 gcagggagct caaaatggag gacgcggcgc tcgggagagc gggcgggtga gtcacccaca
     5701 caaaggaaaa gggcctttcc gtcctcagcc gtcgcttcat gtgactccac ggagtaccgg
     5761 gcgccgtcca ggcacctcga ttagttctcg agcttttgga gtacgtcgtc tttaggttgg
     5821 ggggaggggt tttatgcgat ggagtttccc cacactgagt gggtggagac tgaagttagg
     5881 ccagcttggc acttgatgta attctccttg gaatttgccc tttttgagtt tggatcttgg
     5941 ttcattctca agcctcagac agtggttcaa agtttttttc ttccatttca ggtgtcgtga
     6001 ggaattctgc agtcgatcga cggtaccgcg ggcccgggat ccacggtcgc caccatgggc
     6061 tacccatacg atgtgccaga ttacgctgga ggaggcgggg gagggaagga gaccgccgcc
     6121 gccaagttcg agaggcagca catggacagc ggaggaggcg ggggagggta tacagatatc
     6181 gaaatgaata gactcggaaa gggcatgctg atggtgagca agggcgagga ggataacatg
     6241 gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt gaacggccac
     6301 gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag
     6361 ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc ccctcagttc
     6421 atgtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta cttgaagctg
     6481 tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg
     6541 accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt gaagctgcgc
     6601 ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg ctgggaggcc
     6661 tcctccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa gcagaggctg
     6721 aagctgaagg acggcggcca ctacgacgct gaggtcaaga ccacctacaa ggccaagaag
     6781 cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt tggacatcac ctcccacaac
     6841 gaggactaca ccatcgtgga acagtacgaa cgcgccgagg gccgccactc caccggcggc
     6901 atggacgagc tgtacaagaa acggccagcc gaggatatgg aggaagaaca agcctttaaa
     6961 cgatctcgat aaagcggccg cgtcgacaat caacctctgg attacaaaat ttgtgaaaga
     7021 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     7081 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     7141 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     7201 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     7261 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     7321 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     7381 aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     7441 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     7501 ccggctctgc ggcctcttcc gcatcttcgc cttcgccctc agacgagtcg gatctccctt
     7561 tgggccgcct ccccgcctgg gtacctttaa gaccaatgac ttacaaggca gctgtagatc
     7621 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagat
     7681 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     7741 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     7801 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     7861 agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat
     7921 aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg
     7981 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     8041 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc
     8101 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     8161 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     8221 ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt
     8281 gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     8341 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     8401 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac
     8461 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     8521 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     8581 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     8641 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     8701 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     8761 ctcttgttcc aaactggaac aacactcaac
//
