LOCUS       sequence-353130-        8799 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables spatial detection of 
            CRISPR screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8799)
  AUTHORS   Dhainaut M, Rose SA, Akturk G, Wroblewska A, Nielsen SR, Park ES, 
            Buckup M, Roudko V, Pia L, Sweeney R, Le Berichel J, Wilk CM, 
            Bektesevic A, Lee BH, Bhardwaj N, Rahman AH, Baccarini A, Gnjatic S,
            Pe'er D, Merad M, Brown BD
  TITLE     Spatial CRISPR genomics identifies regulators of the tumor 
            microenvironment.
  JOURNAL   Cell. 2022 Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015.
  PUBMED    35290801
REFERENCE   2  (bases 1 to 8799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2022 
            Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015."
COMMENT     Sequence Label: pLKO_HA.S.V5_mCherry-NLS
FEATURES             Location/Qualifiers
     source          1..8799
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             join(105..173,174..965)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(323..342)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1136..1724
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1625..1644
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1878..1895
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2012..2033
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2048..2053,2054..2071,2072..2078)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2086..2102
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2091..2113
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2110..2126
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2110..2126
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2145..2165
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2147..2165
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2193..2419
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2420..2600
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2647..2772
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3265..3498
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3683..3727
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        4025..4273
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     4025..4045
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     4196..4215
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        4292..4367
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    4415..4532
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4672..5850
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4903..5841
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5798..5818
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      5899..5908
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5911..5937
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5956..6000
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             6019..6060
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6070..6777
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6215..6233)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6483..6502
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    6847..7435
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6900..6920)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7318..7329)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7510..7743
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7815..7936
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7852..7871)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7906..7925
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7976..8111
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8131..8150)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8132..8150)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8160..8177)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8160..8176)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8169..8191)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8318..8773
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8405..8424)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8615..8636
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        join(8799,1..104)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
       61 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
      121 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
      181 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
      241 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
      301 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
      361 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
      421 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
      481 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
      541 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
      601 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
      661 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
      721 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
      781 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
      841 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
      901 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
      961 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     1021 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     1081 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     1141 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     1201 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     1261 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     1321 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     1381 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     1441 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     1501 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     1561 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     1621 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     1681 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     1741 cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat
     1801 cccctgattc tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca
     1861 gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca
     1921 aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     1981 actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     2041 cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     2101 aatttcacac aggaaacagc tatgaccatg attacgccaa gcgcgcaatt aaccctcact
     2161 aaagggaaca aaagctggag ctgcaagctt aatgtagtct tatgcaatac tcttgtagtc
     2221 ttgcaacatg gtaacgatga gttagcaaca tgccttacaa ggagagaaaa agcaccgtgc
     2281 atggcgattg gtggaagtaa ggtggtacga tcgtgcctta ttaggaaggc aacagacggg
     2341 tctgacatgg attggacgaa ccactgaatt gccgcattgc agagatattg tatttaagtg
     2401 cctagctcga tacataaacg ggtctctctg gttagaccag atctgagcct gggagctctc
     2461 tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt
     2521 agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc
     2581 agtgtggaaa atctctagca gtggcgcccg aacagggact tgaaagcgaa agggaaacca
     2641 gaggagctct ctcgacgcag gactcggctt gctgaagcgc gcacggcaag aggcgagggg
     2701 cggcgactgg tgagtacgcc aaaaattttg actagcggag gctagaagga gagagatggg
     2761 tgcgagagcg tcagtattaa gcgggggaga attagatcgc gatgggaaaa aattcggtta
     2821 aggccagggg gaaagaaaaa atataaatta aaacatatag tatgggcaag cagggagcta
     2881 gaacgattcg cagttaatcc tggcctgtta gaaacatcag aaggctgtag acaaatactg
     2941 ggacagctac aaccatccct tcagacagga tcagaagaac ttagatcatt atataataca
     3001 gtagcaaccc tctattgtgt gcatcaaagg atagagataa aagacaccaa ggaagcttta
     3061 gacaagatag aggaagagca aaacaaaagt aagaccaccg cacagcaagc ggccgctgat
     3121 cttcagacct ggaggaggag atatgaggga caattggaga agtgaattat ataaatataa
     3181 agtagtaaaa attgaaccat taggagtagc acccaccaag gcaaagagaa gagtggtgca
     3241 gagagaaaaa agagcagtgg gaataggagc tttgttcctt gggttcttgg gagcagcagg
     3301 aagcactatg ggcgcagcgt caatgacgct gacggtacag gccagacaat tattgtctgg
     3361 tatagtgcag cagcagaaca atttgctgag ggctattgag gcgcaacagc atctgttgca
     3421 actcacagtc tggggcatca agcagctcca ggcaagaatc ctggctgtgg aaagatacct
     3481 aaaggatcaa cagctcctgg ggatttgggg ttgctctgga aaactcattt gcaccactgc
     3541 tgtgccttgg aatgctagtt ggagtaataa atctctggaa cagatttgga atcacacgac
     3601 ctggatggag tgggacagag aaattaacaa ttacacaagc ttaatacact ccttaattga
     3661 agaatcgcaa aaccagcaag aaaagaatga acaagaatta ttggaattag ataaatgggc
     3721 aagtttgtgg aattggttta acataacaaa ttggctgtgg tatataaaat tattcataat
     3781 gatagtagga ggcttggtag gtttaagaat agtttttgct gtactttcta tagtgaatag
     3841 agttaggcag ggatattcac cattatcgtt tcagacccac ctcccaaccc cgaggggacc
     3901 cgacaggccc gaaggaatag aagaagaagg tggagagaga gacagagaca gatccattcg
     3961 attagtgaac ggatctcgac ggtatcgatc acgagactag tatcgagcgg ccgccccctt
     4021 caccgagggc ctatttccca tgattccttc atatttgcat atacgataca aggctgttag
     4081 agagataatt ggaattaatt tgactgtaaa cacaaagata ttagtacaaa atacgtgacg
     4141 tagaaagtaa taatttcttg ggtagtttgc agttttaaaa ttatgtttta aaatggacta
     4201 tcatatgctt accgtaactt gaaagtattt cgatttcttg gctttatata tcttgtggaa
     4261 aggacgaaac accgggtctt cgagaagacc tgttttagag ctagaaatag caagttaaaa
     4321 taaggctagt ccgttatcaa cttgaaaaag tggcaccgag tcggtgcttt ttttaattct
     4381 cgacctcgag acaaatggca gtattcatcc acaattttaa aagaaaaggg gggattgggg
     4441 ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa actaaagaat
     4501 tacaaaaaca aattacaaaa attcaaaatt ttcgggttta ttacagggac agcagagatc
     4561 cactttggcc gcggctcgag gcgataagct ttgcaaagat ggataaagtt ttaaacagag
     4621 aggaatcttt gcagctaatg gaccttctag gtcttgaaag gagtgggaat tggctccggt
     4681 gcccgtcagt gggcagagcg cacatcgccc acagtccccg agaagttggg gggaggggtc
     4741 ggcaattgaa ccggtgccta gagaaggtgg cgcggggtaa actgggaaag tgatgtcgtg
     4801 tactggctcc gcctttttcc cgagggtggg ggagaaccgt atataagtgc agtagtcgcc
     4861 gtgaacgttc tttttcgcaa cgggtttgcc gccagaacac aggtaagtgc cgtgtgtggt
     4921 tcccgcgggc ctggcctctt tacgggttat ggcccttgcg tgccttgaat tacttccacc
     4981 tggctgcagt acgtgattct tgatcccgag cttcgggttg gaagtgggtg ggagagttcg
     5041 aggccttgcg cttaaggagc cccttcgcct cgtgcttgag ttgaggcctg gcctgggcgc
     5101 tggggccgcc gcgtgcgaat ctggtggcac cttcgcgcct gtctcgctgc tttcgataag
     5161 tctctagcca tttaaaattt ttgatgacct gctgcgacgc tttttttctg gcaagatagt
     5221 cttgtaaatg cgggccaaga tctgcacact ggtatttcgg tttttggggc cgcgggcggc
     5281 gacggggccc gtgcgtccca gcgcacatgt tcggcgaggc ggggcctgcg agcgcggcca
     5341 ccgagaatcg gacgggggta gtctcaagct ggccggcctg ctctggtgcc tggcctcgcg
     5401 ccgccgtgta tcgccccgcc ctgggcggca aggctggccc ggtcggcacc agttgcgtga
     5461 gcggaaagat ggccgcttcc cggccctgct gcagggagct caaaatggag gacgcggcgc
     5521 tcgggagagc gggcgggtga gtcacccaca caaaggaaaa gggcctttcc gtcctcagcc
     5581 gtcgcttcat gtgactccac ggagtaccgg gcgccgtcca ggcacctcga ttagttctcg
     5641 agcttttgga gtacgtcgtc tttaggttgg ggggaggggt tttatgcgat ggagtttccc
     5701 cacactgagt gggtggagac tgaagttagg ccagcttggc acttgatgta attctccttg
     5761 gaatttgccc tttttgagtt tggatcttgg ttcattctca agcctcagac agtggttcaa
     5821 agtttttttc ttccatttca ggtgtcgtga ggaattctgc agtcgatcga cggtaccgcg
     5881 ggcccgggat ccacggtcgc caccatgggc tacccatacg atgtgccaga ttacgctgga
     5941 ggaggcgggg gagggaagga gaccgccgcc gccaagttcg agaggcagca catggacagc
     6001 ggaggaggcg ggggaggggg caaaccaatt ccaaacccac tgctgggact ggattccaca
     6061 ggcatgctga tggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg
     6121 cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag
     6181 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc
     6241 cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac
     6301 gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag
     6361 tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc
     6421 ctgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac
     6481 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc
     6541 gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac
     6601 tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc
     6661 tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa
     6721 cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagaaa
     6781 cggccagccg aggatatgga ggaagaacaa gcctttaaac gatctcgata aagcggccgc
     6841 gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     6901 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     6961 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     7021 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     7081 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     7141 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     7201 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg
     7261 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     7321 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     7381 catcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctggg
     7441 tacctttaag accaatgact tacaaggcag ctgtagatct tagccacttt ttaaaagaaa
     7501 aggggggact ggaagggcta attcactccc aacgaagata agatctgctt tttgcttgta
     7561 ctgggtctct ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc
     7621 cactgcttaa gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt
     7681 tgtgtgactc tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta
     7741 gcagtagtag ttcatgtcat cttattattc agtatttata acttgcaaag aaatgaatat
     7801 cagagagtga gaggaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     7861 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     7921 tcatcaatgt atcttatcat gtctggctct agctatcccg cccctaactc cgcccatccc
     7981 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
     8041 ttatgcagag gccgaggccg cctcggcctc tgagctattc cagaagtagt gaggaggctt
     8101 ttttggaggc ctagggacgt acccaattcg ccctatagtg agtcgtatta cgcgcgctca
     8161 ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc
     8221 cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg caccgatcgc
     8281 ccttcccaac agttgcgcag cctgaatggc gaatgggacg cgccctgtag cggcgcatta
     8341 agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     8401 cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     8461 gctctaaatc gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc
     8521 aaaaaacttg attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     8581 cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     8641 acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     8701 tattggttaa aaaatgagct gatttaacaa aaatttaacg cgaattttaa caaaatatta
     8761 acgcttacaa tttaggtggc acttttcggg gaaatgtgc
//
