LOCUS       sequence-353131-        8787 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables spatial detection of 
            CRISPR screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8787)
  AUTHORS   Dhainaut M, Rose SA, Akturk G, Wroblewska A, Nielsen SR, Park ES, 
            Buckup M, Roudko V, Pia L, Sweeney R, Le Berichel J, Wilk CM, 
            Bektesevic A, Lee BH, Bhardwaj N, Rahman AH, Baccarini A, Gnjatic S,
            Pe'er D, Merad M, Brown BD
  TITLE     Spatial CRISPR genomics identifies regulators of the tumor 
            microenvironment.
  JOURNAL   Cell. 2022 Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015.
  PUBMED    35290801
REFERENCE   2  (bases 1 to 8787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2022 
            Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015."
COMMENT     Sequence Label: pLKO_HA.VSVg.V5_mCherry-NLS
FEATURES             Location/Qualifiers
     source          1..8787
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             14..55
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             65..772
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(210..228)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     478..497
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    842..1430
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(895..915)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(1313..1324)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             1505..1738
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1810..1931
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1847..1866)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1901..1920
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1971..2106
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(2126..2145)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2127..2145)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2155..2172)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2155..2171)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2164..2186)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2313..2768
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2400..2419)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2610..2631
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2794..2898
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2899..2967,2968..3759)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3117..3136)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3930..4518
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4419..4438
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4672..4689
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4806..4827
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4842..4847,4848..4865,4866..4872)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4880..4896
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4885..4907
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4904..4920
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4904..4920
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4939..4959
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4941..4959
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4987..5213
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             5214..5394
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5441..5566
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6059..6292
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6477..6521
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        6819..7067
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     6819..6839
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     6990..7009
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        7086..7161
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    7209..7326
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7466..8644
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          7697..8635
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     8592..8612
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      8693..8702
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8705..8731
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             8750..8782
                     /codon_start=1
                     /product="epitope tag from vesicular stomatitis virus G 
                     protein"
                     /label=VSV-G tag
                     /translation="YTDIEMNRLGK"
ORIGIN
        1 aggcggggga gggggcaaac caattccaaa cccactgctg ggactggatt ccacaggcat
       61 gctgatggtg agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt
      121 caaggtgcac atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga
      181 gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct
      241 gcccttcgcc tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa
      301 gcaccccgcc gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga
      361 gcgcgtgatg aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca
      421 ggacggcgag ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc
      481 cgtaatgcag aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga
      541 cggcgccctg aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga
      601 cgctgaggtc aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa
      661 cgtcaacatc aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta
      721 cgaacgcgcc gagggccgcc actccaccgg cggcatggac gagctgtaca agaaacggcc
      781 agccgaggat atggaggaag aacaagcctt taaacgatct cgataaagcg gccgcgtcga
      841 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
      901 tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg
      961 tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt
     1021 gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac
     1081 tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc
     1141 tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct
     1201 gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct
     1261 cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct
     1321 caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcatct
     1381 tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc ctgggtacct
     1441 ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg
     1501 ggactggaag ggctaattca ctcccaacga agataagatc tgctttttgc ttgtactggg
     1561 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     1621 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     1681 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     1741 agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag
     1801 agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca
     1861 aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc
     1921 aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc atcccgcccc
     1981 taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt tttatttatg
     2041 cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga ggcttttttg
     2101 gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc gctcactggc
     2161 cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta atcgccttgc
     2221 agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     2281 ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc
     2341 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     2401 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     2461 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     2521 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     2581 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     2641 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     2701 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct
     2761 tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     2821 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     2881 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     2941 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     3001 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     3061 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     3121 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     3181 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     3241 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     3301 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     3361 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     3421 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     3481 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     3541 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     3601 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     3661 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     3721 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     3781 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     3841 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     3901 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     3961 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     4021 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     4081 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     4141 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     4201 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     4261 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     4321 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     4381 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     4441 agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     4501 gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc
     4561 tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga taaccgtatt
     4621 accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg cagcgagtca
     4681 gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg
     4741 attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac
     4801 gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg
     4861 gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac
     4921 catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct ggagctgcaa
     4981 gcttaatgta gtcttatgca atactcttgt agtcttgcaa catggtaacg atgagttagc
     5041 aacatgcctt acaaggagag aaaaagcacc gtgcatggcg attggtggaa gtaaggtggt
     5101 acgatcgtgc cttattagga aggcaacaga cgggtctgac atggattgga cgaaccactg
     5161 aattgccgca ttgcagagat attgtattta agtgcctagc tcgatacata aacgggtctc
     5221 tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta
     5281 agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact
     5341 ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagtggcg
     5401 cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac gcaggactcg
     5461 gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta cgccaaaaat
     5521 tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta ttaagcgggg
     5581 gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga aaaaatataa
     5641 attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta atcctggcct
     5701 gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat cccttcagac
     5761 aggatcagaa gaacttagat cattatataa tacagtagca accctctatt gtgtgcatca
     5821 aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag agcaaaacaa
     5881 aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga ggagatatga
     5941 gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa ccattaggag
     6001 tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca gtgggaatag
     6061 gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca gcgtcaatga
     6121 cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag aacaatttgc
     6181 tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc atcaagcagc
     6241 tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc ctggggattt
     6301 ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct agttggagta
     6361 ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac agagaaatta
     6421 acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag caagaaaaga
     6481 atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg tttaacataa
     6541 caaattggct gtggtatata aaattattca taatgatagt aggaggcttg gtaggtttaa
     6601 gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat tcaccattat
     6661 cgtttcagac ccacctccca accccgaggg gacccgacag gcccgaagga atagaagaag
     6721 aaggtggaga gagagacaga gacagatcca ttcgattagt gaacggatct cgacggtatc
     6781 gatcacgaga ctagtatcga gcggccgccc ccttcaccga gggcctattt cccatgattc
     6841 cttcatattt gcatatacga tacaaggctg ttagagagat aattggaatt aatttgactg
     6901 taaacacaaa gatattagta caaaatacgt gacgtagaaa gtaataattt cttgggtagt
     6961 ttgcagtttt aaaattatgt tttaaaatgg actatcatat gcttaccgta acttgaaagt
     7021 atttcgattt cttggcttta tatatcttgt ggaaaggacg aaacaccggg tcttcgagaa
     7081 gacctgtttt agagctagaa atagcaagtt aaaataaggc tagtccgtta tcaacttgaa
     7141 aaagtggcac cgagtcggtg ctttttttaa ttctcgacct cgagacaaat ggcagtattc
     7201 atccacaatt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta
     7261 gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa
     7321 aattttcggg tttattacag ggacagcaga gatccacttt ggccgcggct cgaggcgata
     7381 agctttgcaa agatggataa agttttaaac agagaggaat ctttgcagct aatggacctt
     7441 ctaggtcttg aaaggagtgg gaattggctc cggtgcccgt cagtgggcag agcgcacatc
     7501 gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaaccggtg cctagagaag
     7561 gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt ttcccgaggg
     7621 tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc gcaacgggtt
     7681 tgccgccaga acacaggtaa gtgccgtgtg tggttcccgc gggcctggcc tctttacggg
     7741 ttatggccct tgcgtgcctt gaattacttc cacctggctg cagtacgtga ttcttgatcc
     7801 cgagcttcgg gttggaagtg ggtgggagag ttcgaggcct tgcgcttaag gagccccttc
     7861 gcctcgtgct tgagttgagg cctggcctgg gcgctggggc cgccgcgtgc gaatctggtg
     7921 gcaccttcgc gcctgtctcg ctgctttcga taagtctcta gccatttaaa atttttgatg
     7981 acctgctgcg acgctttttt tctggcaaga tagtcttgta aatgcgggcc aagatctgca
     8041 cactggtatt tcggtttttg gggccgcggg cggcgacggg gcccgtgcgt cccagcgcac
     8101 atgttcggcg aggcggggcc tgcgagcgcg gccaccgaga atcggacggg ggtagtctca
     8161 agctggccgg cctgctctgg tgcctggcct cgcgccgccg tgtatcgccc cgccctgggc
     8221 ggcaaggctg gcccggtcgg caccagttgc gtgagcggaa agatggccgc ttcccggccc
     8281 tgctgcaggg agctcaaaat ggaggacgcg gcgctcggga gagcgggcgg gtgagtcacc
     8341 cacacaaagg aaaagggcct ttccgtcctc agccgtcgct tcatgtgact ccacggagta
     8401 ccgggcgccg tccaggcacc tcgattagtt ctcgagcttt tggagtacgt cgtctttagg
     8461 ttggggggag gggttttatg cgatggagtt tccccacact gagtgggtgg agactgaagt
     8521 taggccagct tggcacttga tgtaattctc cttggaattt gccctttttg agtttggatc
     8581 ttggttcatt ctcaagcctc agacagtggt tcaaagtttt tttcttccat ttcaggtgtc
     8641 gtgaggaatt ctgcagtcga tcgacggtac cgcgggcccg ggatccacgg tcgccaccat
     8701 gggctaccca tacgatgtgc cagattacgc tggaggaggc gggggagggt atacagatat
     8761 cgaaatgaat agactcggaa agggagg
//
