LOCUS       sequence-353133-        8790 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables spatial detection of 
            CRISPR screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8790)
  AUTHORS   Dhainaut M, Rose SA, Akturk G, Wroblewska A, Nielsen SR, Park ES, 
            Buckup M, Roudko V, Pia L, Sweeney R, Le Berichel J, Wilk CM, 
            Bektesevic A, Lee BH, Bhardwaj N, Rahman AH, Baccarini A, Gnjatic S,
            Pe'er D, Merad M, Brown BD
  TITLE     Spatial CRISPR genomics identifies regulators of the tumor 
            microenvironment.
  JOURNAL   Cell. 2022 Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015.
  PUBMED    35290801
REFERENCE   2  (bases 1 to 8790)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2022 
            Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015."
COMMENT     Sequence Label: pLKO_HSV.NWS.S_mCherry-NLS
FEATURES             Location/Qualifiers
     source          1..8790
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        56..160
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(161..229,230..1021)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(379..398)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1192..1780
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1681..1700
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1934..1951
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2068..2089
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2104..2109,2110..2127,2128..2134)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2142..2158
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2147..2169
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2166..2182
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2166..2182
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2201..2221
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2203..2221
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2249..2475
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2476..2656
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2703..2828
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3321..3554
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3739..3783
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        4081..4329
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     4081..4101
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     4252..4271
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        4348..4423
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    4471..4588
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4728..5906
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4959..5897
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5854..5874
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      5955..5964
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5967..5999
                     /codon_start=1
                     /product="HSV (herpes simplex virus) epitope tag"
                     /label=HSV tag
                     /translation="QPELAPEDPED"
     CDS             6021..6044
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             6063..6107
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             6117..6824
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6262..6280)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6530..6549
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    6894..7482
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6947..6967)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7365..7376)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7557..7790
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7862..7983
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7899..7918)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7953..7972
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8023..8158
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8178..8197)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8179..8197)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8207..8224)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8207..8223)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8216..8238)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      join(8365..8790,1..3)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8452..8471)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8662..8683
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa atgtgcgcgg
       61 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
      121 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
      181 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
      241 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
      301 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
      361 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg ccgggcaaga
      421 gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact caccagtcac
      481 agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg ccataaccat
      541 gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga aggagctaac
      601 cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg aaccggagct
      661 gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa tggcaacaac
      721 gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac aattaataga
      781 ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc cggctggctg
      841 gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca ttgcagcact
      901 ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga gtcaggcaac
      961 tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta agcattggta
     1021 actgtcagac caagtttact catatatact ttagattgat ttaaaacttc atttttaatt
     1081 taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga
     1141 gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc
     1201 tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt
     1261 ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc
     1321 gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc
     1381 tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg
     1441 cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg
     1501 gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga
     1561 actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc
     1621 ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg
     1681 gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg
     1741 atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt
     1801 tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc
     1861 tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg
     1921 aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa tacgcaaacc
     1981 gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt ttcccgactg
     2041 gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt aggcacccca
     2101 ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg gataacaatt
     2161 tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc ctcactaaag
     2221 ggaacaaaag ctggagctgc aagcttaatg tagtcttatg caatactctt gtagtcttgc
     2281 aacatggtaa cgatgagtta gcaacatgcc ttacaaggag agaaaaagca ccgtgcatgg
     2341 cgattggtgg aagtaaggtg gtacgatcgt gccttattag gaaggcaaca gacgggtctg
     2401 acatggattg gacgaaccac tgaattgccg cattgcagag atattgtatt taagtgccta
     2461 gctcgataca taaacgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     2521 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     2581 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     2641 tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg aaaccagagg
     2701 agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc gaggggcggc
     2761 gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga gatgggtgcg
     2821 agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt cggttaaggc
     2881 cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg gagctagaac
     2941 gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa atactgggac
     3001 agctacaacc atcccttcag acaggatcag aagaacttag atcattatat aatacagtag
     3061 caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa gctttagaca
     3121 agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc gctgatcttc
     3181 agacctggag gaggagatat gagggacaat tggagaagtg aattatataa atataaagta
     3241 gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt ggtgcagaga
     3301 gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc agcaggaagc
     3361 actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt gtctggtata
     3421 gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct gttgcaactc
     3481 acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag atacctaaag
     3541 gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac cactgctgtg
     3601 ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca cacgacctgg
     3661 atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt aattgaagaa
     3721 tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa atgggcaagt
     3781 ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt cataatgata
     3841 gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt gaatagagtt
     3901 aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag gggacccgac
     3961 aggcccgaag gaatagaaga agaaggtgga gagagagaca gagacagatc cattcgatta
     4021 gtgaacggat ctcgacggta tcgatcacga gactagtatc gagcggccgc ccccttcacc
     4081 gagggcctat ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag
     4141 ataattggaa ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga
     4201 aagtaataat ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat
     4261 atgcttaccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
     4321 cgaaacaccg ggtcttcgag aagacctgtt ttagagctag aaatagcaag ttaaaataag
     4381 gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcttttttt aattctcgac
     4441 ctcgagacaa atggcagtat tcatccacaa ttttaaaaga aaagggggga ttggggggta
     4501 cagtgcaggg gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca
     4561 aaaacaaatt acaaaaattc aaaattttcg ggtttattac agggacagca gagatccact
     4621 ttggccgcgg ctcgaggcga taagctttgc aaagatggat aaagttttaa acagagagga
     4681 atctttgcag ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc
     4741 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
     4801 attgaaccgg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
     4861 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
     4921 acgttctttt tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc
     4981 gcgggcctgg cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc
     5041 tgcagtacgt gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc
     5101 cttgcgctta aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg
     5161 gccgccgcgt gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc
     5221 tagccattta aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg
     5281 taaatgcggg ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg
     5341 gggcccgtgc gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga
     5401 gaatcggacg ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc
     5461 cgtgtatcgc cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg
     5521 aaagatggcc gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg
     5581 gagagcgggc gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg
     5641 cttcatgtga ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct
     5701 tttggagtac gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca
     5761 ctgagtgggt ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat
     5821 ttgccctttt tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt
     5881 tttttcttcc atttcaggtg tcgtgaggaa ttctgcagtc gatcgacggt accgcgggcc
     5941 cgggatccac ggtcgccacc atgggccagc ccgagctggc ccccgaggac cccgaggacg
     6001 gaggaggcgg gggagggaac tggtcccacc cccaattcga gaaaggagga ggcgggggag
     6061 ggaaggagac cgccgccgcc aagttcgaga ggcagcacat ggacagcggc atgctgatgg
     6121 tgagcaaggg cgaggaggat aacatggcca tcatcaagga gttcatgcgc ttcaaggtgc
     6181 acatggaggg ctccgtgaac ggccacgagt tcgagatcga gggcgagggc gagggccgcc
     6241 cctacgaggg cacccagacc gccaagctga aggtgaccaa gggtggcccc ctgcccttcg
     6301 cctgggacat cctgtcccct cagttcatgt acggctccaa ggcctacgtg aagcaccccg
     6361 ccgacatccc cgactacttg aagctgtcct tccccgaggg cttcaagtgg gagcgcgtga
     6421 tgaacttcga ggacggcggc gtggtgaccg tgacccagga ctcctccctg caggacggcg
     6481 agttcatcta caaggtgaag ctgcgcggca ccaacttccc ctccgacggc cccgtaatgc
     6541 agaagaagac catgggctgg gaggcctcct ccgagcggat gtaccccgag gacggcgccc
     6601 tgaagggcga gatcaagcag aggctgaagc tgaaggacgg cggccactac gacgctgagg
     6661 tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc cggcgcctac aacgtcaaca
     6721 tcaagttgga catcacctcc cacaacgagg actacaccat cgtggaacag tacgaacgcg
     6781 ccgagggccg ccactccacc ggcggcatgg acgagctgta caagaaacgg ccagccgagg
     6841 atatggagga agaacaagcc tttaaacgat ctcgataaag cggccgcgtc gacaatcaac
     6901 ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta
     6961 cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt
     7021 tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg
     7081 ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg
     7141 gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca
     7201 cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca
     7261 ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg
     7321 ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag
     7381 cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcat cttcgccttc
     7441 gccctcagac gagtcggatc tccctttggg ccgcctcccc gcctgggtac ctttaagacc
     7501 aatgacttac aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga
     7561 agggctaatt cactcccaac gaagataaga tctgcttttt gcttgtactg ggtctctctg
     7621 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
     7681 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
     7741 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc
     7801 atgtcatctt attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag
     7861 gaacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     7921 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     7981 ttatcatgtc tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg
     8041 cccagttccg cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc
     8101 gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta
     8161 gggacgtacc caattcgccc tatagtgagt cgtattacgc gcgctcactg gccgtcgttt
     8221 tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
     8281 cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
     8341 tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     8401 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     8461 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     8521 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     8581 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     8641 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     8701 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     8761 atgagctgat ttaacaaaaa tttaacgcga
//
