LOCUS       sequence-353135-        8787 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables spatial detection of 
            CRISPR screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8787)
  AUTHORS   Dhainaut M, Rose SA, Akturk G, Wroblewska A, Nielsen SR, Park ES, 
            Buckup M, Roudko V, Pia L, Sweeney R, Le Berichel J, Wilk CM, 
            Bektesevic A, Lee BH, Bhardwaj N, Rahman AH, Baccarini A, Gnjatic S,
            Pe'er D, Merad M, Brown BD
  TITLE     Spatial CRISPR genomics identifies regulators of the tumor 
            microenvironment.
  JOURNAL   Cell. 2022 Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015.
  PUBMED    35290801
REFERENCE   2  (bases 1 to 8787)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2022 
            Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015."
COMMENT     Sequence Label: pLKO_HSV.NWS.V5_mCherry-NLS
FEATURES             Location/Qualifiers
     source          1..8787
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    279..400
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(316..335)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     370..389
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      440..575
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(595..614)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(596..614)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(624..641)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(624..640)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(633..655)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      782..1237
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(869..888)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1079..1100
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1263..1367
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1368..1436,1437..2228)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1586..1605)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2399..2987
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2888..2907
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3141..3158
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3275..3296
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3311..3316,3317..3334,3335..3341)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3349..3365
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3354..3376
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3373..3389
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3373..3389
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3408..3428
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3410..3428
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        3456..3682
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             3683..3863
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3910..4035
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4528..4761
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4946..4990
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        5288..5536
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     5288..5308
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     5459..5478
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        5555..5630
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    5678..5795
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5935..7113
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6166..7104
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     7061..7081
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      7162..7171
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7174..7206
                     /codon_start=1
                     /product="HSV (herpes simplex virus) epitope tag"
                     /label=HSV tag
                     /translation="QPELAPEDPED"
     CDS             7228..7251
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             7270..7311
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             7321..8028
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(7466..7484)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7734..7753
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    8098..8686
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8151..8171)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8569..8580)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             join(8761..8787,1..207)
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
ORIGIN
        1 gataagatct gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg
       61 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
      121 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
      181 tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt
      241 tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata
      301 atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc
      361 attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat
      421 cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc
      481 ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct
      541 attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat
      601 agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac
      661 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
      721 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
      781 gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc
      841 gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc
      901 acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt
      961 agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg
     1021 ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt
     1081 ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta
     1141 taagggattt tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt
     1201 aacgcgaatt ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg
     1261 tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     1321 gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     1381 atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     1441 cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     1501 tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     1561 caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg
     1621 ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     1681 cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     1741 taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     1801 agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     1861 cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg
     1921 caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     1981 taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     2041 ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     2101 cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     2161 aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     2221 attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     2281 tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     2341 aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt
     2401 gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag
     2461 cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta actggcttca
     2521 gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca
     2581 agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg
     2641 ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg
     2701 cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct
     2761 acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaaggga
     2821 gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc
     2881 ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg
     2941 agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg
     3001 cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt
     3061 tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc
     3121 gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac
     3181 gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc
     3241 ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg
     3301 caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat
     3361 aacaatttca cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc
     3421 actaaaggga acaaaagctg gagctgcaag cttaatgtag tcttatgcaa tactcttgta
     3481 gtcttgcaac atggtaacga tgagttagca acatgcctta caaggagaga aaaagcaccg
     3541 tgcatggcga ttggtggaag taaggtggta cgatcgtgcc ttattaggaa ggcaacagac
     3601 gggtctgaca tggattggac gaaccactga attgccgcat tgcagagata ttgtatttaa
     3661 gtgcctagct cgatacataa acgggtctct ctggttagac cagatctgag cctgggagct
     3721 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     3781 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     3841 gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg acttgaaagc gaaagggaaa
     3901 ccagaggagc tctctcgacg caggactcgg cttgctgaag cgcgcacggc aagaggcgag
     3961 gggcggcgac tggtgagtac gccaaaaatt ttgactagcg gaggctagaa ggagagagat
     4021 gggtgcgaga gcgtcagtat taagcggggg agaattagat cgcgatggga aaaaattcgg
     4081 ttaaggccag ggggaaagaa aaaatataaa ttaaaacata tagtatgggc aagcagggag
     4141 ctagaacgat tcgcagttaa tcctggcctg ttagaaacat cagaaggctg tagacaaata
     4201 ctgggacagc tacaaccatc ccttcagaca ggatcagaag aacttagatc attatataat
     4261 acagtagcaa ccctctattg tgtgcatcaa aggatagaga taaaagacac caaggaagct
     4321 ttagacaaga tagaggaaga gcaaaacaaa agtaagacca ccgcacagca agcggccgct
     4381 gatcttcaga cctggaggag gagatatgag ggacaattgg agaagtgaat tatataaata
     4441 taaagtagta aaaattgaac cattaggagt agcacccacc aaggcaaaga gaagagtggt
     4501 gcagagagaa aaaagagcag tgggaatagg agctttgttc cttgggttct tgggagcagc
     4561 aggaagcact atgggcgcag cgtcaatgac gctgacggta caggccagac aattattgtc
     4621 tggtatagtg cagcagcaga acaatttgct gagggctatt gaggcgcaac agcatctgtt
     4681 gcaactcaca gtctggggca tcaagcagct ccaggcaaga atcctggctg tggaaagata
     4741 cctaaaggat caacagctcc tggggatttg gggttgctct ggaaaactca tttgcaccac
     4801 tgctgtgcct tggaatgcta gttggagtaa taaatctctg gaacagattt ggaatcacac
     4861 gacctggatg gagtgggaca gagaaattaa caattacaca agcttaatac actccttaat
     4921 tgaagaatcg caaaaccagc aagaaaagaa tgaacaagaa ttattggaat tagataaatg
     4981 ggcaagtttg tggaattggt ttaacataac aaattggctg tggtatataa aattattcat
     5041 aatgatagta ggaggcttgg taggtttaag aatagttttt gctgtacttt ctatagtgaa
     5101 tagagttagg cagggatatt caccattatc gtttcagacc cacctcccaa ccccgagggg
     5161 acccgacagg cccgaaggaa tagaagaaga aggtggagag agagacagag acagatccat
     5221 tcgattagtg aacggatctc gacggtatcg atcacgagac tagtatcgag cggccgcccc
     5281 cttcaccgag ggcctatttc ccatgattcc ttcatatttg catatacgat acaaggctgt
     5341 tagagagata attggaatta atttgactgt aaacacaaag atattagtac aaaatacgtg
     5401 acgtagaaag taataatttc ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga
     5461 ctatcatatg cttaccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg
     5521 gaaaggacga aacaccgggt cttcgagaag acctgtttta gagctagaaa tagcaagtta
     5581 aaataaggct agtccgttat caacttgaaa aagtggcacc gagtcggtgc tttttttaat
     5641 tctcgacctc gagacaaatg gcagtattca tccacaattt taaaagaaaa ggggggattg
     5701 gggggtacag tgcaggggaa agaatagtag acataatagc aacagacata caaactaaag
     5761 aattacaaaa acaaattaca aaaattcaaa attttcgggt ttattacagg gacagcagag
     5821 atccactttg gccgcggctc gaggcgataa gctttgcaaa gatggataaa gttttaaaca
     5881 gagaggaatc tttgcagcta atggaccttc taggtcttga aaggagtggg aattggctcc
     5941 ggtgcccgtc agtgggcaga gcgcacatcg cccacagtcc ccgagaagtt ggggggaggg
     6001 gtcggcaatt gaaccggtgc ctagagaagg tggcgcgggg taaactggga aagtgatgtc
     6061 gtgtactggc tccgcctttt tcccgagggt gggggagaac cgtatataag tgcagtagtc
     6121 gccgtgaacg ttctttttcg caacgggttt gccgccagaa cacaggtaag tgccgtgtgt
     6181 ggttcccgcg ggcctggcct ctttacgggt tatggccctt gcgtgccttg aattacttcc
     6241 acctggctgc agtacgtgat tcttgatccc gagcttcggg ttggaagtgg gtgggagagt
     6301 tcgaggcctt gcgcttaagg agccccttcg cctcgtgctt gagttgaggc ctggcctggg
     6361 cgctggggcc gccgcgtgcg aatctggtgg caccttcgcg cctgtctcgc tgctttcgat
     6421 aagtctctag ccatttaaaa tttttgatga cctgctgcga cgcttttttt ctggcaagat
     6481 agtcttgtaa atgcgggcca agatctgcac actggtattt cggtttttgg ggccgcgggc
     6541 ggcgacgggg cccgtgcgtc ccagcgcaca tgttcggcga ggcggggcct gcgagcgcgg
     6601 ccaccgagaa tcggacgggg gtagtctcaa gctggccggc ctgctctggt gcctggcctc
     6661 gcgccgccgt gtatcgcccc gccctgggcg gcaaggctgg cccggtcggc accagttgcg
     6721 tgagcggaaa gatggccgct tcccggccct gctgcaggga gctcaaaatg gaggacgcgg
     6781 cgctcgggag agcgggcggg tgagtcaccc acacaaagga aaagggcctt tccgtcctca
     6841 gccgtcgctt catgtgactc cacggagtac cgggcgccgt ccaggcacct cgattagttc
     6901 tcgagctttt ggagtacgtc gtctttaggt tggggggagg ggttttatgc gatggagttt
     6961 ccccacactg agtgggtgga gactgaagtt aggccagctt ggcacttgat gtaattctcc
     7021 ttggaatttg ccctttttga gtttggatct tggttcattc tcaagcctca gacagtggtt
     7081 caaagttttt ttcttccatt tcaggtgtcg tgaggaattc tgcagtcgat cgacggtacc
     7141 gcgggcccgg gatccacggt cgccaccatg ggccagcccg agctggcccc cgaggacccc
     7201 gaggacggag gaggcggggg agggaactgg tcccaccccc aattcgagaa aggaggaggc
     7261 gggggagggg gcaaaccaat tccaaaccca ctgctgggac tggattccac aggcatgctg
     7321 atggtgagca agggcgagga ggataacatg gccatcatca aggagttcat gcgcttcaag
     7381 gtgcacatgg agggctccgt gaacggccac gagttcgaga tcgagggcga gggcgagggc
     7441 cgcccctacg agggcaccca gaccgccaag ctgaaggtga ccaagggtgg ccccctgccc
     7501 ttcgcctggg acatcctgtc ccctcagttc atgtacggct ccaaggccta cgtgaagcac
     7561 cccgccgaca tccccgacta cttgaagctg tccttccccg agggcttcaa gtgggagcgc
     7621 gtgatgaact tcgaggacgg cggcgtggtg accgtgaccc aggactcctc cctgcaggac
     7681 ggcgagttca tctacaaggt gaagctgcgc ggcaccaact tcccctccga cggccccgta
     7741 atgcagaaga agaccatggg ctgggaggcc tcctccgagc ggatgtaccc cgaggacggc
     7801 gccctgaagg gcgagatcaa gcagaggctg aagctgaagg acggcggcca ctacgacgct
     7861 gaggtcaaga ccacctacaa ggccaagaag cccgtgcagc tgcccggcgc ctacaacgtc
     7921 aacatcaagt tggacatcac ctcccacaac gaggactaca ccatcgtgga acagtacgaa
     7981 cgcgccgagg gccgccactc caccggcggc atggacgagc tgtacaagaa acggccagcc
     8041 gaggatatgg aggaagaaca agcctttaaa cgatctcgat aaagcggccg cgtcgacaat
     8101 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     8161 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     8221 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     8281 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     8341 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     8401 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     8461 ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc
     8521 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     8581 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcatcttcgc
     8641 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg gtacctttaa
     8701 gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa aaggggggac
     8761 tggaagggct aattcactcc caacgaa
//
