LOCUS       sequence-353137-        8808 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables spatial detection of 
            CRISPR screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8808)
  AUTHORS   Dhainaut M, Rose SA, Akturk G, Wroblewska A, Nielsen SR, Park ES, 
            Buckup M, Roudko V, Pia L, Sweeney R, Le Berichel J, Wilk CM, 
            Bektesevic A, Lee BH, Bhardwaj N, Rahman AH, Baccarini A, Gnjatic S,
            Pe'er D, Merad M, Brown BD
  TITLE     Spatial CRISPR genomics identifies regulators of the tumor 
            microenvironment.
  JOURNAL   Cell. 2022 Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015.
  PUBMED    35290801
REFERENCE   2  (bases 1 to 8808)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2022 
            Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015."
COMMENT     Sequence Label: pLKO_HSV.Ollas.S_mCherry-NLS
FEATURES             Location/Qualifiers
     source          1..8808
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     119..138
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    483..1071
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(536..556)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(954..965)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             1146..1379
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    1451..1572
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(1488..1507)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1542..1561
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1612..1747
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1767..1786)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1768..1786)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1796..1813)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1796..1812)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1805..1827)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1954..2409
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2041..2060)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2251..2272
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2435..2539
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2540..2608,2609..3400)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2758..2777)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3571..4159
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4060..4079
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     4313..4330
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    4447..4468
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(4483..4488,4489..4506,4507..4513)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4521..4537
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     4526..4548
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4545..4561
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4545..4561
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4580..4600
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4582..4600
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4628..4854
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4855..5035
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5082..5207
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5700..5933
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6118..6162
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        6460..6708
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     6460..6480
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     6631..6650
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        6727..6802
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    6850..6967
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        7107..8285
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          7338..8276
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     8233..8253
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      8334..8343
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             8346..8378
                     /codon_start=1
                     /product="HSV (herpes simplex virus) epitope tag"
                     /label=HSV tag
                     /translation="QPELAPEDPED"
     CDS             8397..8438
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             8460..8504
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             join(8514..8808,1..413)
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(8659..8677)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 agcgcgtgat gaacttcgag gacggcggcg tggtgaccgt gacccaggac tcctccctgc
       61 aggacggcga gttcatctac aaggtgaagc tgcgcggcac caacttcccc tccgacggcc
      121 ccgtaatgca gaagaagacc atgggctggg aggcctcctc cgagcggatg taccccgagg
      181 acggcgccct gaagggcgag atcaagcaga ggctgaagct gaaggacggc ggccactacg
      241 acgctgaggt caagaccacc tacaaggcca agaagcccgt gcagctgccc ggcgcctaca
      301 acgtcaacat caagttggac atcacctccc acaacgagga ctacaccatc gtggaacagt
      361 acgaacgcgc cgagggccgc cactccaccg gcggcatgga cgagctgtac aagaaacggc
      421 cagccgagga tatggaggaa gaacaagcct ttaaacgatc tcgataaagc ggccgcgtcg
      481 acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
      541 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
      601 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
      661 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
      721 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
      781 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
      841 tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc
      901 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
      961 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcatc
     1021 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg cctgggtacc
     1081 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     1141 gggactggaa gggctaattc actcccaacg aagataagat ctgctttttg cttgtactgg
     1201 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     1261 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     1321 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     1381 tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat gaatatcaga
     1441 gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca atagcatcac
     1501 aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat
     1561 caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc catcccgccc
     1621 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
     1681 gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt
     1741 ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg cgctcactgg
     1801 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
     1861 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
     1921 cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg
     1981 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
     2041 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
     2101 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
     2161 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
     2221 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
     2281 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
     2341 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc
     2401 ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     2461 ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     2521 atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     2581 tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     2641 tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     2701 ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     2761 atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca
     2821 ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     2881 catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     2941 cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     3001 ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     3061 cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg
     3121 cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     3181 tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     3241 agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     3301 ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     3361 gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     3421 atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     3481 cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     3541 agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     3601 ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     3661 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     3721 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     3781 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     3841 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     3901 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     3961 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     4021 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     4081 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     4141 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     4201 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     4261 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     4321 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     4381 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     4441 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     4501 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
     4561 ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc tggagctgca
     4621 agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag
     4681 caacatgcct tacaaggaga gaaaaagcac cgtgcatggc gattggtgga agtaaggtgg
     4741 tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact
     4801 gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct
     4861 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     4921 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     4981 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
     5041 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
     5101 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
     5161 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
     5221 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
     5281 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
     5341 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
     5401 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
     5461 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
     5521 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
     5581 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
     5641 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
     5701 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
     5761 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
     5821 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
     5881 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
     5941 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
     6001 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
     6061 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
     6121 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
     6181 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
     6241 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
     6301 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
     6361 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat
     6421 cgatcacgag actagtatcg agcggccgcc cccttcaccg agggcctatt tcccatgatt
     6481 ccttcatatt tgcatatacg atacaaggct gttagagaga taattggaat taatttgact
     6541 gtaaacacaa agatattagt acaaaatacg tgacgtagaa agtaataatt tcttgggtag
     6601 tttgcagttt taaaattatg ttttaaaatg gactatcata tgcttaccgt aacttgaaag
     6661 tatttcgatt tcttggcttt atatatcttg tggaaaggac gaaacaccgg gtcttcgaga
     6721 agacctgttt tagagctaga aatagcaagt taaaataagg ctagtccgtt atcaacttga
     6781 aaaagtggca ccgagtcggt gcttttttta attctcgacc tcgagacaaa tggcagtatt
     6841 catccacaat tttaaaagaa aaggggggat tggggggtac agtgcagggg aaagaatagt
     6901 agacataata gcaacagaca tacaaactaa agaattacaa aaacaaatta caaaaattca
     6961 aaattttcgg gtttattaca gggacagcag agatccactt tggccgcggc tcgaggcgat
     7021 aagctttgca aagatggata aagttttaaa cagagaggaa tctttgcagc taatggacct
     7081 tctaggtctt gaaaggagtg ggaattggct ccggtgcccg tcagtgggca gagcgcacat
     7141 cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaaccggt gcctagagaa
     7201 ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg
     7261 gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt
     7321 ttgccgccag aacacaggta agtgccgtgt gtggttcccg cgggcctggc ctctttacgg
     7381 gttatggccc ttgcgtgcct tgaattactt ccacctggct gcagtacgtg attcttgatc
     7441 ccgagcttcg ggttggaagt gggtgggaga gttcgaggcc ttgcgcttaa ggagcccctt
     7501 cgcctcgtgc ttgagttgag gcctggcctg ggcgctgggg ccgccgcgtg cgaatctggt
     7561 ggcaccttcg cgcctgtctc gctgctttcg ataagtctct agccatttaa aatttttgat
     7621 gacctgctgc gacgcttttt ttctggcaag atagtcttgt aaatgcgggc caagatctgc
     7681 acactggtat ttcggttttt ggggccgcgg gcggcgacgg ggcccgtgcg tcccagcgca
     7741 catgttcggc gaggcggggc ctgcgagcgc ggccaccgag aatcggacgg gggtagtctc
     7801 aagctggccg gcctgctctg gtgcctggcc tcgcgccgcc gtgtatcgcc ccgccctggg
     7861 cggcaaggct ggcccggtcg gcaccagttg cgtgagcgga aagatggccg cttcccggcc
     7921 ctgctgcagg gagctcaaaa tggaggacgc ggcgctcggg agagcgggcg ggtgagtcac
     7981 ccacacaaag gaaaagggcc tttccgtcct cagccgtcgc ttcatgtgac tccacggagt
     8041 accgggcgcc gtccaggcac ctcgattagt tctcgagctt ttggagtacg tcgtctttag
     8101 gttgggggga ggggttttat gcgatggagt ttccccacac tgagtgggtg gagactgaag
     8161 ttaggccagc ttggcacttg atgtaattct ccttggaatt tgcccttttt gagtttggat
     8221 cttggttcat tctcaagcct cagacagtgg ttcaaagttt ttttcttcca tttcaggtgt
     8281 cgtgaggaat tctgcagtcg atcgacggta ccgcgggccc gggatccacg gtcgccacca
     8341 tgggccagcc cgagctggcc cccgaggacc ccgaggacgg aggaggcggg ggagggagcg
     8401 gcttcgccaa cgagctgggc cccaggctga tgggcaagtg cggaggaggc gggggaggga
     8461 aggagaccgc cgccgccaag ttcgagaggc agcacatgga cagcggcatg ctgatggtga
     8521 gcaagggcga ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca
     8581 tggagggctc cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct
     8641 acgagggcac ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct
     8701 gggacatcct gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg
     8761 acatccccga ctacttgaag ctgtccttcc ccgagggctt caagtggg
//
