LOCUS       sequence-353142-        8805 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables spatial detection of 
            CRISPR screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8805)
  AUTHORS   Dhainaut M, Rose SA, Akturk G, Wroblewska A, Nielsen SR, Park ES, 
            Buckup M, Roudko V, Pia L, Sweeney R, Le Berichel J, Wilk CM, 
            Bektesevic A, Lee BH, Bhardwaj N, Rahman AH, Baccarini A, Gnjatic S,
            Pe'er D, Merad M, Brown BD
  TITLE     Spatial CRISPR genomics identifies regulators of the tumor 
            microenvironment.
  JOURNAL   Cell. 2022 Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015.
  PUBMED    35290801
REFERENCE   2  (bases 1 to 8805)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2022 
            Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015."
COMMENT     Sequence Label: pLKO_HSV.S.V5_mCherry-NLS
FEATURES             Location/Qualifiers
     source          1..8805
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        88..192
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(193..261,262..1053)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(411..430)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1224..1812
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1713..1732
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1966..1983
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2100..2121
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2136..2141,2142..2159,2160..2166)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2174..2190
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2179..2201
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2198..2214
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2198..2214
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2233..2253
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2235..2253
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2281..2507
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2508..2688
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2735..2860
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3353..3586
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3771..3815
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        4113..4361
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     4113..4133
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     4284..4303
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        4380..4455
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    4503..4620
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4760..5938
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4991..5929
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5886..5906
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      5987..5996
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5999..6031
                     /codon_start=1
                     /product="HSV (herpes simplex virus) epitope tag"
                     /label=HSV tag
                     /translation="QPELAPEDPED"
     CDS             6050..6094
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             6113..6154
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6164..6871
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6309..6327)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6577..6596
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    6941..7529
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6994..7014)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7412..7423)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7604..7837
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7909..8030
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7946..7965)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8000..8019
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8070..8205
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8225..8244)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8226..8244)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8254..8271)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8254..8270)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8263..8285)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      join(8412..8805,1..62)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8499..8518)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8709..8730
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
       61 ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
      121 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
      181 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
      241 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
      301 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
      361 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
      421 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
      481 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
      541 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
      601 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
      661 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
      721 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
      781 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
      841 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
      901 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
      961 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     1021 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     1081 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     1141 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     1201 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     1261 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     1321 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     1381 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     1441 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     1501 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     1561 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     1621 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     1681 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     1741 gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     1801 agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     1861 tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     1921 tttgagtgag ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc
     1981 gaggaagcgg aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
     2041 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
     2101 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
     2161 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
     2221 tacgccaagc gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagcttaa
     2281 tgtagtctta tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg
     2341 ccttacaagg agagaaaaag caccgtgcat ggcgattggt ggaagtaagg tggtacgatc
     2401 gtgccttatt aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc
     2461 cgcattgcag agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt
     2521 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
     2581 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
     2641 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa
     2701 cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc
     2761 tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac
     2821 tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat
     2881 tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa
     2941 acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga
     3001 aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc
     3061 agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat
     3121 agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa
     3181 gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca
     3241 attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac
     3301 ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt
     3361 tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga
     3421 cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg
     3481 ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg
     3541 caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt
     3601 gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat
     3661 ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt
     3721 acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac
     3781 aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt
     3841 ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag
     3901 tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc
     3961 agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg
     4021 gagagagaga cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgatcac
     4081 gagactagta tcgagcggcc gcccccttca ccgagggcct atttcccatg attccttcat
     4141 atttgcatat acgatacaag gctgttagag agataattgg aattaatttg actgtaaaca
     4201 caaagatatt agtacaaaat acgtgacgta gaaagtaata atttcttggg tagtttgcag
     4261 ttttaaaatt atgttttaaa atggactatc atatgcttac cgtaacttga aagtatttcg
     4321 atttcttggc tttatatatc ttgtggaaag gacgaaacac cgggtcttcg agaagacctg
     4381 ttttagagct agaaatagca agttaaaata aggctagtcc gttatcaact tgaaaaagtg
     4441 gcaccgagtc ggtgcttttt ttaattctcg acctcgagac aaatggcagt attcatccac
     4501 aattttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     4561 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     4621 cgggtttatt acagggacag cagagatcca ctttggccgc ggctcgaggc gataagcttt
     4681 gcaaagatgg ataaagtttt aaacagagag gaatctttgc agctaatgga ccttctaggt
     4741 cttgaaagga gtgggaattg gctccggtgc ccgtcagtgg gcagagcgca catcgcccac
     4801 agtccccgag aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg
     4861 cggggtaaac tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg
     4921 agaaccgtat ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc
     4981 cagaacacag gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg
     5041 cccttgcgtg ccttgaatta cttccacctg gctgcagtac gtgattcttg atcccgagct
     5101 tcgggttgga agtgggtggg agagttcgag gccttgcgct taaggagccc cttcgcctcg
     5161 tgcttgagtt gaggcctggc ctgggcgctg gggccgccgc gtgcgaatct ggtggcacct
     5221 tcgcgcctgt ctcgctgctt tcgataagtc tctagccatt taaaattttt gatgacctgc
     5281 tgcgacgctt tttttctggc aagatagtct tgtaaatgcg ggccaagatc tgcacactgg
     5341 tatttcggtt tttggggccg cgggcggcga cggggcccgt gcgtcccagc gcacatgttc
     5401 ggcgaggcgg ggcctgcgag cgcggccacc gagaatcgga cgggggtagt ctcaagctgg
     5461 ccggcctgct ctggtgcctg gcctcgcgcc gccgtgtatc gccccgccct gggcggcaag
     5521 gctggcccgg tcggcaccag ttgcgtgagc ggaaagatgg ccgcttcccg gccctgctgc
     5581 agggagctca aaatggagga cgcggcgctc gggagagcgg gcgggtgagt cacccacaca
     5641 aaggaaaagg gcctttccgt cctcagccgt cgcttcatgt gactccacgg agtaccgggc
     5701 gccgtccagg cacctcgatt agttctcgag cttttggagt acgtcgtctt taggttgggg
     5761 ggaggggttt tatgcgatgg agtttcccca cactgagtgg gtggagactg aagttaggcc
     5821 agcttggcac ttgatgtaat tctccttgga atttgccctt tttgagtttg gatcttggtt
     5881 cattctcaag cctcagacag tggttcaaag tttttttctt ccatttcagg tgtcgtgagg
     5941 aattctgcag tcgatcgacg gtaccgcggg cccgggatcc acggtcgcca ccatgggcca
     6001 gcccgagctg gcccccgagg accccgagga cggaggaggc gggggaggga aggagaccgc
     6061 cgccgccaag ttcgagaggc agcacatgga cagcggagga ggcgggggag ggggcaaacc
     6121 aattccaaac ccactgctgg gactggattc cacaggcatg ctgatggtga gcaagggcga
     6181 ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc
     6241 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac
     6301 ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct
     6361 gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga
     6421 ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga
     6481 cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa
     6541 ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat
     6601 gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat
     6661 caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta
     6721 caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat
     6781 cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca
     6841 ctccaccggc ggcatggacg agctgtacaa gaaacggcca gccgaggata tggaggaaga
     6901 acaagccttt aaacgatctc gataaagcgg ccgcgtcgac aatcaacctc tggattacaa
     6961 aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata
     7021 cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc
     7081 cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg
     7141 tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac
     7201 ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat
     7261 cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt
     7321 ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg ccacctggat
     7381 tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc
     7441 ccgcggcctg ctgccggctc tgcggcctct tccgcatctt cgccttcgcc ctcagacgag
     7501 tcggatctcc ctttgggccg cctccccgcc tgggtacctt taagaccaat gacttacaag
     7561 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     7621 tcccaacgaa gataagatct gctttttgct tgtactgggt ctctctggtt agaccagatc
     7681 tgagcctggg agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg
     7741 ccttgagtgc ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc
     7801 ctcagaccct tttagtcagt gtggaaaatc tctagcagta gtagttcatg tcatcttatt
     7861 attcagtatt tataacttgc aaagaaatga atatcagaga gtgagaggaa cttgtttatt
     7921 gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa taaagcattt
     7981 ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta tcatgtctgg
     8041 ctctagctat cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     8101 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     8161 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggg acgtacccaa
     8221 ttcgccctat agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga
     8281 ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag
     8341 ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa
     8401 tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg
     8461 cagcgtgacc gctacacttg ccagcgccct agcgcccgct cctttcgctt tcttcccttc
     8521 ctttctcgcc acgttcgccg gctttccccg tcaagctcta aatcgggggc tccctttagg
     8581 gttccgattt agtgctttac ggcacctcga ccccaaaaaa cttgattagg gtgatggttc
     8641 acgtagtggg ccatcgccct gatagacggt ttttcgccct ttgacgttgg agtccacgtt
     8701 ctttaatagt ggactcttgt tccaaactgg aacaacactc aaccctatct cggtctattc
     8761 ttttgattta taagggattt tgccgatttc ggcctattgg ttaaa
//
