LOCUS       sequence-353143-        8802 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables spatial detection of 
            CRISPR screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8802)
  AUTHORS   Dhainaut M, Rose SA, Akturk G, Wroblewska A, Nielsen SR, Park ES, 
            Buckup M, Roudko V, Pia L, Sweeney R, Le Berichel J, Wilk CM, 
            Bektesevic A, Lee BH, Bhardwaj N, Rahman AH, Baccarini A, Gnjatic S,
            Pe'er D, Merad M, Brown BD
  TITLE     Spatial CRISPR genomics identifies regulators of the tumor 
            microenvironment.
  JOURNAL   Cell. 2022 Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015.
  PUBMED    35290801
REFERENCE   2  (bases 1 to 8802)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2022 
            Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015."
COMMENT     Sequence Label: pLKO_NWS.Ollas.S_mCherry-NLS
FEATURES             Location/Qualifiers
     source          1..8802
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    198..319
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(235..254)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     289..308
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      359..494
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(514..533)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(515..533)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(543..560)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(543..559)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(552..574)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      701..1156
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(788..807)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     998..1019
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1182..1286
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1287..1355,1356..2147)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1505..1524)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2318..2906
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     2807..2826
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3060..3077
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3194..3215
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3230..3235,3236..3253,3254..3260)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3268..3284
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3273..3295
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3292..3308
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3292..3308
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     3327..3347
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        3329..3347
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        3375..3601
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             3602..3782
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    3829..3954
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    4447..4680
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             4865..4909
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        5207..5455
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     5207..5227
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     5378..5397
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        5474..5549
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    5597..5714
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        5854..7032
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          6085..7023
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     6980..7000
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      7081..7090
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             7096..7119
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             7138..7179
                     /codon_start=1
                     /product="Escherichia coli OmpF Linker and mouse Langerin 
                     fusion sequence tag (Park et al., 2008)"
                     /label=OLLAS tag
                     /translation="SGFANELGPRLMGK"
     CDS             7201..7245
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             7255..7962
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(7400..7418)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     7668..7687
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    8032..8620
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8085..8105)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(8503..8514)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             join(8695..8802,1..126)
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
ORIGIN
        1 acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc
       61 tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg tggaaaatct
      121 ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca aagaaatgaa
      181 tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa taaagcaata
      241 gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca
      301 aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa ctccgcccat
      361 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
      421 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
      481 cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta ttacgcgcgc
      541 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
      601 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
      661 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
      721 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
      781 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
      841 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
      901 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
      961 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     1021 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     1081 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     1141 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     1201 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     1261 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     1321 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     1381 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     1441 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     1501 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     1561 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     1621 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     1681 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     1741 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     1801 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     1861 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     1921 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     1981 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     2041 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     2101 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     2161 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     2221 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     2281 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     2341 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     2401 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     2461 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     2521 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     2581 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     2641 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     2701 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     2761 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     2821 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     2881 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     2941 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     3001 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     3061 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     3121 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     3181 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     3241 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     3301 gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg
     3361 agctgcaagc ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat
     3421 gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatggcgat tggtggaagt
     3481 aaggtggtac gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg
     3541 aaccactgaa ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa
     3601 cgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     3661 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     3721 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     3781 cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc
     3841 aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg
     3901 ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt
     3961 aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa
     4021 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
     4081 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
     4141 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
     4201 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
     4261 caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg
     4321 agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc
     4381 attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt
     4441 gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc
     4501 gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa
     4561 caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat
     4621 caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct
     4681 ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag
     4741 ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag
     4801 agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca
     4861 agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt
     4921 taacataaca aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt
     4981 aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc
     5041 accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat
     5101 agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg
     5161 acggtatcga tcacgagact agtatcgagc ggccgccccc ttcaccgagg gcctatttcc
     5221 catgattcct tcatatttgc atatacgata caaggctgtt agagagataa ttggaattaa
     5281 tttgactgta aacacaaaga tattagtaca aaatacgtga cgtagaaagt aataatttct
     5341 tgggtagttt gcagttttaa aattatgttt taaaatggac tatcatatgc ttaccgtaac
     5401 ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgaa acaccgggtc
     5461 ttcgagaaga cctgttttag agctagaaat agcaagttaa aataaggcta gtccgttatc
     5521 aacttgaaaa agtggcaccg agtcggtgct ttttttaatt ctcgacctcg agacaaatgg
     5581 cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa
     5641 gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa
     5701 aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccactttgg ccgcggctcg
     5761 aggcgataag ctttgcaaag atggataaag ttttaaacag agaggaatct ttgcagctaa
     5821 tggaccttct aggtcttgaa aggagtggga attggctccg gtgcccgtca gtgggcagag
     5881 cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg aaccggtgcc
     5941 tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt
     6001 cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc
     6061 aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg gcctggcctc
     6121 tttacgggtt atggcccttg cgtgccttga attacttcca cctggctgca gtacgtgatt
     6181 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga
     6241 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga
     6301 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat
     6361 ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa
     6421 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc
     6481 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg
     6541 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg
     6601 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt
     6661 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt
     6721 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc
     6781 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg
     6841 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag
     6901 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag
     6961 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt
     7021 caggtgtcgt gaggaattct gcagtcgatc gacggtaccg cgggcccggg atccacggtc
     7081 gccaccatgg gcaactggtc ccacccccaa ttcgagaaag gaggaggcgg gggagggagc
     7141 ggcttcgcca acgagctggg ccccaggctg atgggcaagt gcggaggagg cgggggaggg
     7201 aaggagaccg ccgccgccaa gttcgagagg cagcacatgg acagcggcat gctgatggtg
     7261 agcaagggcg aggaggataa catggccatc atcaaggagt tcatgcgctt caaggtgcac
     7321 atggagggct ccgtgaacgg ccacgagttc gagatcgagg gcgagggcga gggccgcccc
     7381 tacgagggca cccagaccgc caagctgaag gtgaccaagg gtggccccct gcccttcgcc
     7441 tgggacatcc tgtcccctca gttcatgtac ggctccaagg cctacgtgaa gcaccccgcc
     7501 gacatccccg actacttgaa gctgtccttc cccgagggct tcaagtggga gcgcgtgatg
     7561 aacttcgagg acggcggcgt ggtgaccgtg acccaggact cctccctgca ggacggcgag
     7621 ttcatctaca aggtgaagct gcgcggcacc aacttcccct ccgacggccc cgtaatgcag
     7681 aagaagacca tgggctggga ggcctcctcc gagcggatgt accccgagga cggcgccctg
     7741 aagggcgaga tcaagcagag gctgaagctg aaggacggcg gccactacga cgctgaggtc
     7801 aagaccacct acaaggccaa gaagcccgtg cagctgcccg gcgcctacaa cgtcaacatc
     7861 aagttggaca tcacctccca caacgaggac tacaccatcg tggaacagta cgaacgcgcc
     7921 gagggccgcc actccaccgg cggcatggac gagctgtaca agaaacggcc agccgaggat
     7981 atggaggaag aacaagcctt taaacgatct cgataaagcg gccgcgtcga caatcaacct
     8041 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     8101 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     8161 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
     8221 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
     8281 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
     8341 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
     8401 gacaattccg tggtgttgtc ggggaagctg acgtcctttc catggctgct cgcctgtgtt
     8461 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
     8521 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcatct tcgccttcgc
     8581 cctcagacga gtcggatctc cctttgggcc gcctccccgc ctgggtacct ttaagaccaa
     8641 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
     8701 ggctaattca ctcccaacga agataagatc tgctttttgc ttgtactggg tctctctggt
     8761 tagaccagat ctgagcctgg gagctctctg gctaactagg ga
//
