LOCUS       sequence-353147-        8799 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables spatial detection of 
            CRISPR screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8799)
  AUTHORS   Dhainaut M, Rose SA, Akturk G, Wroblewska A, Nielsen SR, Park ES, 
            Buckup M, Roudko V, Pia L, Sweeney R, Le Berichel J, Wilk CM, 
            Bektesevic A, Lee BH, Bhardwaj N, Rahman AH, Baccarini A, Gnjatic S,
            Pe'er D, Merad M, Brown BD
  TITLE     Spatial CRISPR genomics identifies regulators of the tumor 
            microenvironment.
  JOURNAL   Cell. 2022 Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015.
  PUBMED    35290801
REFERENCE   2  (bases 1 to 8799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2022 
            Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015."
COMMENT     Sequence Label: pLKO_NWS.S.V5_mCherry-NLS
FEATURES             Location/Qualifiers
     source          1..8799
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        67..171
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(172..240,241..1032)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(390..409)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1203..1791
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1692..1711
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1945..1962
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2079..2100
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2115..2120,2121..2138,2139..2145)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2153..2169
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2158..2180
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2177..2193
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2177..2193
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2212..2232
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2214..2232
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2260..2486
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2487..2667
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2714..2839
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3332..3565
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3750..3794
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        4092..4340
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     4092..4112
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     4263..4282
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        4359..4434
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    4482..4599
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4739..5917
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4970..5908
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5865..5885
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      5966..5975
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             5981..6004
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             6023..6067
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     CDS             6086..6127
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6137..6844
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6282..6300)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6550..6569
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    6914..7502
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6967..6987)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7385..7396)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7577..7810
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7882..8003
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7919..7938)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7973..7992
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8043..8178
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8198..8217)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8199..8217)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8227..8244)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8227..8243)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8236..8258)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      join(8385..8799,1..41)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8472..8491)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8682..8703
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 atttaacgcg aattttaaca aaatattaac gcttacaatt taggtggcac ttttcgggga
       61 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
      121 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
      181 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
      241 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
      301 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
      361 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
      421 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
      481 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
      541 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
      601 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
      661 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
      721 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
      781 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
      841 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
      901 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
      961 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
     1021 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
     1081 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
     1141 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
     1201 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
     1261 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
     1321 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
     1381 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
     1441 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
     1501 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
     1561 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
     1621 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
     1681 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
     1741 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
     1801 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gttctttcct
     1861 gcgttatccc ctgattctgt ggataaccgt attaccgcct ttgagtgagc tgataccgct
     1921 cgccgcagcc gaacgaccga gcgcagcgag tcagtgagcg aggaagcgga agagcgccca
     1981 atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg
     2041 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
     2101 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
     2161 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagcg cgcaattaac
     2221 cctcactaaa gggaacaaaa gctggagctg caagcttaat gtagtcttat gcaatactct
     2281 tgtagtcttg caacatggta acgatgagtt agcaacatgc cttacaagga gagaaaaagc
     2341 accgtgcatg gcgattggtg gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac
     2401 agacgggtct gacatggatt ggacgaacca ctgaattgcc gcattgcaga gatattgtat
     2461 ttaagtgcct agctcgatac ataaacgggt ctctctggtt agaccagatc tgagcctggg
     2521 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
     2581 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     2641 tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg
     2701 gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg
     2761 cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag
     2821 agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat
     2881 tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag
     2941 ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca
     3001 aatactggga cagctacaac catcccttca gacaggatca gaagaactta gatcattata
     3061 taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga
     3121 agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc
     3181 cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata
     3241 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag
     3301 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag
     3361 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat
     3421 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc
     3481 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa
     3541 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca
     3601 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc
     3661 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct
     3721 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata
     3781 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat
     3841 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag
     3901 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga
     3961 ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat
     4021 ccattcgatt agtgaacgga tctcgacggt atcgatcacg agactagtat cgagcggccg
     4081 cccccttcac cgagggccta tttcccatga ttccttcata tttgcatata cgatacaagg
     4141 ctgttagaga gataattgga attaatttga ctgtaaacac aaagatatta gtacaaaata
     4201 cgtgacgtag aaagtaataa tttcttgggt agtttgcagt tttaaaatta tgttttaaaa
     4261 tggactatca tatgcttacc gtaacttgaa agtatttcga tttcttggct ttatatatct
     4321 tgtggaaagg acgaaacacc gggtcttcga gaagacctgt tttagagcta gaaatagcaa
     4381 gttaaaataa ggctagtccg ttatcaactt gaaaaagtgg caccgagtcg gtgctttttt
     4441 taattctcga cctcgagaca aatggcagta ttcatccaca attttaaaag aaaagggggg
     4501 attggggggt acagtgcagg ggaaagaata gtagacataa tagcaacaga catacaaact
     4561 aaagaattac aaaaacaaat tacaaaaatt caaaattttc gggtttatta cagggacagc
     4621 agagatccac tttggccgcg gctcgaggcg ataagctttg caaagatgga taaagtttta
     4681 aacagagagg aatctttgca gctaatggac cttctaggtc ttgaaaggag tgggaattgg
     4741 ctccggtgcc cgtcagtggg cagagcgcac atcgcccaca gtccccgaga agttgggggg
     4801 aggggtcggc aattgaaccg gtgcctagag aaggtggcgc ggggtaaact gggaaagtga
     4861 tgtcgtgtac tggctccgcc tttttcccga gggtggggga gaaccgtata taagtgcagt
     4921 agtcgccgtg aacgttcttt ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt
     4981 gtgtggttcc cgcgggcctg gcctctttac gggttatggc ccttgcgtgc cttgaattac
     5041 ttccacctgg ctgcagtacg tgattcttga tcccgagctt cgggttggaa gtgggtggga
     5101 gagttcgagg ccttgcgctt aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc
     5161 tgggcgctgg ggccgccgcg tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt
     5221 cgataagtct ctagccattt aaaatttttg atgacctgct gcgacgcttt ttttctggca
     5281 agatagtctt gtaaatgcgg gccaagatct gcacactggt atttcggttt ttggggccgc
     5341 gggcggcgac ggggcccgtg cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc
     5401 gcggccaccg agaatcggac gggggtagtc tcaagctggc cggcctgctc tggtgcctgg
     5461 cctcgcgccg ccgtgtatcg ccccgccctg ggcggcaagg ctggcccggt cggcaccagt
     5521 tgcgtgagcg gaaagatggc cgcttcccgg ccctgctgca gggagctcaa aatggaggac
     5581 gcggcgctcg ggagagcggg cgggtgagtc acccacacaa aggaaaaggg cctttccgtc
     5641 ctcagccgtc gcttcatgtg actccacgga gtaccgggcg ccgtccaggc acctcgatta
     5701 gttctcgagc ttttggagta cgtcgtcttt aggttggggg gaggggtttt atgcgatgga
     5761 gtttccccac actgagtggg tggagactga agttaggcca gcttggcact tgatgtaatt
     5821 ctccttggaa tttgcccttt ttgagtttgg atcttggttc attctcaagc ctcagacagt
     5881 ggttcaaagt ttttttcttc catttcaggt gtcgtgagga attctgcagt cgatcgacgg
     5941 taccgcgggc ccgggatcca cggtcgccac catgggcaac tggtcccacc cccaattcga
     6001 gaaaggagga ggcgggggag ggaaggagac cgccgccgcc aagttcgaga ggcagcacat
     6061 ggacagcgga ggaggcgggg gagggggcaa accaattcca aacccactgc tgggactgga
     6121 ttccacaggc atgctgatgg tgagcaaggg cgaggaggat aacatggcca tcatcaagga
     6181 gttcatgcgc ttcaaggtgc acatggaggg ctccgtgaac ggccacgagt tcgagatcga
     6241 gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga aggtgaccaa
     6301 gggtggcccc ctgcccttcg cctgggacat cctgtcccct cagttcatgt acggctccaa
     6361 ggcctacgtg aagcaccccg ccgacatccc cgactacttg aagctgtcct tccccgaggg
     6421 cttcaagtgg gagcgcgtga tgaacttcga ggacggcggc gtggtgaccg tgacccagga
     6481 ctcctccctg caggacggcg agttcatcta caaggtgaag ctgcgcggca ccaacttccc
     6541 ctccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcct ccgagcggat
     6601 gtaccccgag gacggcgccc tgaagggcga gatcaagcag aggctgaagc tgaaggacgg
     6661 cggccactac gacgctgagg tcaagaccac ctacaaggcc aagaagcccg tgcagctgcc
     6721 cggcgcctac aacgtcaaca tcaagttgga catcacctcc cacaacgagg actacaccat
     6781 cgtggaacag tacgaacgcg ccgagggccg ccactccacc ggcggcatgg acgagctgta
     6841 caagaaacgg ccagccgagg atatggagga agaacaagcc tttaaacgat ctcgataaag
     6901 cggccgcgtc gacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
     6961 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
     7021 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
     7081 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
     7141 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
     7201 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
     7261 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt
     7321 tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
     7381 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
     7441 tcttccgcat cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
     7501 gcctgggtac ctttaagacc aatgacttac aaggcagctg tagatcttag ccacttttta
     7561 aaagaaaagg ggggactgga agggctaatt cactcccaac gaagataaga tctgcttttt
     7621 gcttgtactg ggtctctctg gttagaccag atctgagcct gggagctctc tggctaacta
     7681 gggaacccac tgcttaagcc tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc
     7741 cgtctgttgt gtgactctgg taactagaga tccctcagac ccttttagtc agtgtggaaa
     7801 atctctagca gtagtagttc atgtcatctt attattcagt atttataact tgcaaagaaa
     7861 tgaatatcag agagtgagag gaacttgttt attgcagctt ataatggtta caaataaagc
     7921 aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg
     7981 tccaaactca tcaatgtatc ttatcatgtc tggctctagc tatcccgccc ctaactccgc
     8041 ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt
     8101 tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag
     8161 gaggcttttt tggaggccta gggacgtacc caattcgccc tatagtgagt cgtattacgc
     8221 gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg ttacccaact
     8281 taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag aggcccgcac
     8341 cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tgggacgcgc cctgtagcgg
     8401 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     8461 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     8521 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     8581 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     8641 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     8701 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     8761 ttcggcctat tggttaaaaa atgagctgat ttaacaaaa
//
