LOCUS       sequence-353149-        8778 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Nuclear Protein Barcode (nPC) for vector/cell tracking. Contains U6 
            trRNA cassette for sgRNA cloning. Enables spatial detection of 
            CRISPR screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8778)
  AUTHORS   Dhainaut M, Rose SA, Akturk G, Wroblewska A, Nielsen SR, Park ES, 
            Buckup M, Roudko V, Pia L, Sweeney R, Le Berichel J, Wilk CM, 
            Bektesevic A, Lee BH, Bhardwaj N, Rahman AH, Baccarini A, Gnjatic S,
            Pe'er D, Merad M, Brown BD
  TITLE     Spatial CRISPR genomics identifies regulators of the tumor 
            microenvironment.
  JOURNAL   Cell. 2022 Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015.
  PUBMED    35290801
REFERENCE   2  (bases 1 to 8778)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2022 
            Mar 10. pii: S0092-8674(22)00195-7. doi: 
            10.1016/j.cell.2022.02.015."
COMMENT     Sequence Label: pLKO_NWS.HA.V5_mCherry-NLS
FEATURES             Location/Qualifiers
     source          1..8778
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        42..146
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(147..215,216..1007)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(365..384)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      1178..1766
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1667..1686
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1920..1937
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    2054..2075
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(2090..2095,2096..2113,2114..2120)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2128..2144
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2133..2155
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     2152..2168
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     2152..2168
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     2187..2207
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        2189..2207
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        2235..2461
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             2462..2642
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    2689..2814
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    3307..3540
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             3725..3769
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        4067..4315
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     4067..4087
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     4238..4257
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        4334..4409
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     misc_feature    4457..4574
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        4714..5892
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4945..5883
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5840..5860
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             5953..5976
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             5995..6021
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6040..6081
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             6091..6798
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(6236..6254)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     6504..6523
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     misc_feature    6868..7456
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6921..6941)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7339..7350)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7531..7764
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    7836..7957
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7873..7892)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7927..7946
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      7997..8132
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8152..8171)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8153..8171)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8181..8198)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8181..8197)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8190..8212)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8339..8767
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8426..8445)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8636..8657
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
       61 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
      121 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
      181 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
      241 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
      301 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
      361 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
      421 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
      481 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
      541 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
      601 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
      661 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
      721 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
      781 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
      841 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
      901 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
      961 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     1021 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     1081 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     1141 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     1201 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     1261 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     1321 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     1381 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     1441 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     1501 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     1561 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     1621 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     1681 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     1741 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     1801 ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat tctgtggata
     1861 accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg accgagcgca
     1921 gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct ctccccgcgc
     1981 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcagtg
     2041 agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct ttacacttta
     2101 tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac acaggaaaca
     2161 gctatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg
     2221 agctgcaagc ttaatgtagt cttatgcaat actcttgtag tcttgcaaca tggtaacgat
     2281 gagttagcaa catgccttac aaggagagaa aaagcaccgt gcatggcgat tggtggaagt
     2341 aaggtggtac gatcgtgcct tattaggaag gcaacagacg ggtctgacat ggattggacg
     2401 aaccactgaa ttgccgcatt gcagagatat tgtatttaag tgcctagctc gatacataaa
     2461 cgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     2521 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     2581 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     2641 cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct ctctcgacgc
     2701 aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact ggtgagtacg
     2761 ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag cgtcagtatt
     2821 aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg gggaaagaaa
     2881 aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt cgcagttaat
     2941 cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct acaaccatcc
     3001 cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac cctctattgt
     3061 gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat agaggaagag
     3121 caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac ctggaggagg
     3181 agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa aaattgaacc
     3241 attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa aaagagcagt
     3301 gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta tgggcgcagc
     3361 gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc agcagcagaa
     3421 caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag tctggggcat
     3481 caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc aacagctcct
     3541 ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt ggaatgctag
     3601 ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg agtgggacag
     3661 agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc aaaaccagca
     3721 agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt ggaattggtt
     3781 taacataaca aattggctgt ggtatataaa attattcata atgatagtag gaggcttggt
     3841 aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc agggatattc
     3901 accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc ccgaaggaat
     3961 agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga acggatctcg
     4021 acggtatcga tcacgagact agtatcgagc ggccgccccc ttcaccgagg gcctatttcc
     4081 catgattcct tcatatttgc atatacgata caaggctgtt agagagataa ttggaattaa
     4141 tttgactgta aacacaaaga tattagtaca aaatacgtga cgtagaaagt aataatttct
     4201 tgggtagttt gcagttttaa aattatgttt taaaatggac tatcatatgc ttaccgtaac
     4261 ttgaaagtat ttcgatttct tggctttata tatcttgtgg aaaggacgaa acaccgggtc
     4321 ttcgagaaga cctgttttag agctagaaat agcaagttaa aataaggcta gtccgttatc
     4381 aacttgaaaa agtggcaccg agtcggtgct ttttttaatt ctcgacctcg agacaaatgg
     4441 cagtattcat ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa
     4501 gaatagtaga cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa
     4561 aaattcaaaa ttttcgggtt tattacaggg acagcagaga tccactttgg ccgcggctcg
     4621 aggcgataag ctttgcaaag atggataaag ttttaaacag agaggaatct ttgcagctaa
     4681 tggaccttct aggtcttgaa aggagtggga attggctccg gtgcccgtca gtgggcagag
     4741 cgcacatcgc ccacagtccc cgagaagttg gggggagggg tcggcaattg aaccggtgcc
     4801 tagagaaggt ggcgcggggt aaactgggaa agtgatgtcg tgtactggct ccgccttttt
     4861 cccgagggtg ggggagaacc gtatataagt gcagtagtcg ccgtgaacgt tctttttcgc
     4921 aacgggtttg ccgccagaac acaggtaagt gccgtgtgtg gttcccgcgg gcctggcctc
     4981 tttacgggtt atggcccttg cgtgccttga attacttcca cctggctgca gtacgtgatt
     5041 cttgatcccg agcttcgggt tggaagtggg tgggagagtt cgaggccttg cgcttaagga
     5101 gccccttcgc ctcgtgcttg agttgaggcc tggcctgggc gctggggccg ccgcgtgcga
     5161 atctggtggc accttcgcgc ctgtctcgct gctttcgata agtctctagc catttaaaat
     5221 ttttgatgac ctgctgcgac gctttttttc tggcaagata gtcttgtaaa tgcgggccaa
     5281 gatctgcaca ctggtatttc ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc
     5341 cagcgcacat gttcggcgag gcggggcctg cgagcgcggc caccgagaat cggacggggg
     5401 tagtctcaag ctggccggcc tgctctggtg cctggcctcg cgccgccgtg tatcgccccg
     5461 ccctgggcgg caaggctggc ccggtcggca ccagttgcgt gagcggaaag atggccgctt
     5521 cccggccctg ctgcagggag ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt
     5581 gagtcaccca cacaaaggaa aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc
     5641 acggagtacc gggcgccgtc caggcacctc gattagttct cgagcttttg gagtacgtcg
     5701 tctttaggtt ggggggaggg gttttatgcg atggagtttc cccacactga gtgggtggag
     5761 actgaagtta ggccagcttg gcacttgatg taattctcct tggaatttgc cctttttgag
     5821 tttggatctt ggttcattct caagcctcag acagtggttc aaagtttttt tcttccattt
     5881 caggtgtcgt gaggaattct gcagtcgatc gacggtaccg cgggcccggg atccacggtc
     5941 gccaccatga actggtccca cccccaattc gagaaaggag gaggcggggg agggtaccca
     6001 tacgatgtgc cagattacgc tggaggaggc gggggagggg gcaaaccaat tccaaaccca
     6061 ctgctgggac tggattccac aggcatgctg atggtgagca agggcgagga ggataacatg
     6121 gccatcatca aggagttcat gcgcttcaag gtgcacatgg agggctccgt gaacggccac
     6181 gagttcgaga tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag
     6241 ctgaaggtga ccaagggtgg ccccctgccc ttcgcctggg acatcctgtc ccctcagttc
     6301 atgtacggct ccaaggccta cgtgaagcac cccgccgaca tccccgacta cttgaagctg
     6361 tccttccccg agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggcgtggtg
     6421 accgtgaccc aggactcctc cctgcaggac ggcgagttca tctacaaggt gaagctgcgc
     6481 ggcaccaact tcccctccga cggccccgta atgcagaaga agaccatggg ctgggaggcc
     6541 tcctccgagc ggatgtaccc cgaggacggc gccctgaagg gcgagatcaa gcagaggctg
     6601 aagctgaagg acggcggcca ctacgacgct gaggtcaaga ccacctacaa ggccaagaag
     6661 cccgtgcagc tgcccggcgc ctacaacgtc aacatcaagt tggacatcac ctcccacaac
     6721 gaggactaca ccatcgtgga acagtacgaa cgcgccgagg gccgccactc caccggcggc
     6781 atggacgagc tgtacaagaa acggccagcc gaggatatgg aggaagaaca agcctttaaa
     6841 cgatctcgat aaagcggccg cgtcgacaat caacctctgg attacaaaat ttgtgaaaga
     6901 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg
     6961 cctttgtatc atgctattgc ttcccgtatg gctttcattt tctcctcctt gtataaatcc
     7021 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc
     7081 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccacctg tcagctcctt
     7141 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcatcgc cgcctgcctt
     7201 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg
     7261 aagctgacgt cctttccatg gctgctcgcc tgtgttgcca cctggattct gcgcgggacg
     7321 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg
     7381 ccggctctgc ggcctcttcc gcatcttcgc cttcgccctc agacgagtcg gatctccctt
     7441 tgggccgcct ccccgcctgg gtacctttaa gaccaatgac ttacaaggca gctgtagatc
     7501 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagat
     7561 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     7621 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     7681 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     7741 agtcagtgtg gaaaatctct agcagtagta gttcatgtca tcttattatt cagtatttat
     7801 aacttgcaaa gaaatgaata tcagagagtg agaggaactt gtttattgca gcttataatg
     7861 gttacaaata aagcaatagc atcacaaatt tcacaaataa agcatttttt tcactgcatt
     7921 ctagttgtgg tttgtccaaa ctcatcaatg tatcttatca tgtctggctc tagctatccc
     7981 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     8041 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     8101 ccagaagtag tgaggaggct tttttggagg cctagggacg tacccaattc gccctatagt
     8161 gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     8221 ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     8281 gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatgggac
     8341 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     8401 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     8461 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     8521 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     8581 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     8641 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     8701 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     8761 gcgaatttta acaaaata
//
