LOCUS       sequence-353152-       11774 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EGFP fused to the N-terminus of POLB, linked by T2A to N-terminus 
            Myc-tagged SIRT6 with the mutation Gly60Ala, a mutation in the PAM 
            site used by SIRT6-KO gRNA1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11774)
  AUTHORS   Koczor CA, Saville KM, Andrews JF, Clark J, Fang Q, Li J, 
            Al-Rahahleh RQ, Ibrahim M, McClellan S, Makarov MV, Migaud ME, Sobol
            RW
  TITLE     Temporal dynamics of base excision/single-strand break repair 
            protein complex assembly/disassembly are modulated by the 
            PARP/NAD(+)/SIRT6 axis.
  JOURNAL   Cell Rep. 2021 Nov 2;37(5):109917. doi: 
            10.1016/j.celrep.2021.109917.
  PUBMED    34731617
REFERENCE   2  (bases 1 to 11774)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-11-2"; volume: "37(5):109917. doi"; pages: " 
            10.1016/j.celrep.2021.109917"
COMMENT     Sequence Label: pLV-Hygro-EF1a-EGFP-PolB-T2A-Myc-SIRT6(PAMmut-...
FEATURES             Location/Qualifiers
     source          1..11774
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1803..1920
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1938..1958
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        1959..3137
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2190..3128
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3085..3105
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3137..3161
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      3162..3171
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3168..3170,3171..3173,3174..3884)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3168..3170,3171..3173,3174..3884)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3213..3234)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3474..3493)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3821..3842
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4899..4952
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     protein_bind    complement(6021..6045)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    6063..6651
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6116..6136)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6534..6545)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        6682..7181
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(6717..6740)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     7114..7133
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             7198..8223
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             7198..8223
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     LTR             8295..8528
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8600..8721
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8637..8656)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8691..8710
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8761..8896
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8916..8935)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8917..8935)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8945..8962)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8945..8961)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8954..8976)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      9103..9558
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9190..9209)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9400..9421
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9584..9688
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9689..9757,9758..10549)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9907..9926)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10720..11308
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11209..11228
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11462..11479
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11596..11617
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11632..11637,11638..11655,11656..11662)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11670..11686
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11675..11697
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11694..11710
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11694..11710
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11729..11749
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11731..11749
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtgatt atcggatcaa ctttgtatag aaaagttggg ctccggtgcc cgtcagtggg
     1981 cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaaccg
     2041 gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc
     2101 tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt
     2161 ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg
     2221 gcctctttac gggttatggc ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg
     2281 tgattcttga tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt
     2341 aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg
     2401 tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt
     2461 aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg
     2521 gccaagatct gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg
     2581 cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac
     2641 gggggtagtc tcaagctggc cggcctgctc tggtgcctgg tctcgcgccg ccgtgtatcg
     2701 ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc
     2761 cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg
     2821 cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg
     2881 actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta
     2941 cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg
     3001 tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt
     3061 ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc
     3121 catttcaggt gtcgtgacaa gtttgtacaa aaaagcaggc tgccaccatg gtgagcaagg
     3181 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     3241 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     3301 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
     3361 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
     3421 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     3481 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     3541 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     3601 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
     3661 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
     3721 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
     3781 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     3841 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagatgagc aaacggaagg
     3901 cgccgcagga gactctcaac gggggaatca ccgacatgct cacagaactc gcaaactttg
     3961 agaagaacgt gagccaagct atccacaagt acaatgctta cagaaaagca gcatctgtta
     4021 tagcaaaata cccacacaaa ataaagagtg gagctgaagc taagaaattg cctggagtag
     4081 gaacaaaaat tgctgaaaag attgatgagt ttttagcaac tggaaaatta cgtaaactgg
     4141 aaaagattcg gcaggatgat acgagttcat ccatcaattt cctgactcga gttagtggca
     4201 ttggtccatc tgctgcaagg aagtttgtag atgaaggaat taaaacacta gaagatctca
     4261 gaaaaaatga agataaattg aaccatcatc agcgaattgg gctgaaatat tttggggact
     4321 ttgaaaaaag aattcctcgt gaagagatgt tacaaatgca agatattgta ctaaatgaag
     4381 ttaaaaaagt ggattctgaa tacattgcta cagtctgtgg cagtttcaga agaggtgcag
     4441 agtccagtgg tgacatggat gttctcctga cccatcccag cttcacttca gaatcaacca
     4501 aacagccaaa actgttacat caggttgtgg agcagttaca aaaggttcat tttatcacag
     4561 ataccctgtc aaagggtgag acaaagttca tgggtgtttg ccagcttccc agtaaaaatg
     4621 atgaaaaaga atatccacac agaagaattg atatcaggtt gatacccaaa gatcagtatt
     4681 actgtggtgt tctctatttc actgggagtg atattttcaa taagaatatg agggctcatg
     4741 ccctagaaaa gggtttcaca atcaatgagt acaccatccg tcccttggga gtcactggag
     4801 ttgcaggaga acccctgcca gtggatagtg aaaaagacat ctttgattac atccagtgga
     4861 aataccggga acccaaggac cggagcgaag gaagcggaga gggcagggga agtcttctaa
     4921 catgcgggga cgtggaggaa aatcccggcc ccatgtcggt gaattacgca gcagggctgt
     4981 cgccgtacgc ggacaagggc aagtgcggcc tcccggagat cttcgacccc ccggaggagc
     5041 tggagcggaa ggtgtgggaa ctggcgaggc tggtctggca gtcttccagt gtggtgttcc
     5101 acacgggtgc cggcatcagc actgcctctg ccatccccga cttcaggggt ccccacggag
     5161 tctggaccat ggaggagcga ggtctggccc ccaagttcga caccaccttt gagagcgcgc
     5221 ggcccacgca gacccacatg gcgctggtgc agctggagcg cgtgggcctc ctccgcttcc
     5281 tggtcagcca gaacgtggac gggctccatg tgcgctcagg cttccccagg gacaaactgg
     5341 cagagctcca cgggaacatg tttgtggaag aatgtgccaa gtgtaagacg cagtacgtcc
     5401 gagacacagt cgtgggcacc atgggcctga aggccacggg ccggctctgc accgtggcta
     5461 aggcaagggg gctgcgagcc tgcaggggag agctgaggga caccatccta gactgggagg
     5521 actccctgcc cgaccgggac ctggcactcg ccgatgaggc cagcaggaac gccgacctgt
     5581 ccatcacgct gggtacatcg ctgcagatcc ggcccagcgg gaacctgccg ctggctacca
     5641 agcgccgggg aggccgcctg gtcatcgtca acctgcagcc caccaagcac gaccgccatg
     5701 ctgacctccg catccatggc tacgttgacg aggtcatgac ccggctcatg aagcacctgg
     5761 ggctggagat ccccgcctgg gacggccccc gtgtgctgga gagggcgctg ccacccctgc
     5821 cccgcccgcc cacccccaag ctggagccca aggaggaatc tcccacccgg atcaacggct
     5881 ctatccccgc cggccccaag caggagccct gcgcccagca caacggctca gagcccgcca
     5941 gccccaaacg ggagcggccc accagccctg ccccccacag accccccaaa agggtgaagg
     6001 ccaaggcggt ccccagctga acccagcttt cttgtacaaa gtggtgataa tcgaattccg
     6061 ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg
     6121 ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc
     6181 gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt
     6241 tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca
     6301 ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc
     6361 ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     6421 tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt ccatggctgc
     6481 tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc
     6541 tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc
     6601 ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgggaat
     6661 tcccgcggtt cgaattctac cgggtagggg aggcgctttt cccaaggcag tctggagcat
     6721 gcgctttagc agccccgctg ggcacttggc gctacacaag tggcctctgg cctcgcacac
     6781 attccacatc caccggtagg cgccaaccgg ctccgttctt tggtggcccc ttcgcgccac
     6841 cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg tcgtgcagga
     6901 cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc accgctgagc
     6961 aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc ttcgctttct
     7021 gggctcagag gctgggaagg ggtgggtccg ggggcgggct caggggcggg ctcaggggcg
     7081 gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa aagcgcacgt
     7141 ctgccgcgct gttctcctct tcctcatctc cgggcctttc gacctcacgt ggccaccatg
     7201 aaaaagcctg aactcaccgc gacgtctgtc gagaagtttc tgatcgaaaa gttcgacagc
     7261 gtctccgacc tgatgcagct ctcggagggc gaagaatctc gtgctttcag cttcgatgta
     7321 ggagggcgtg gatatgtcct gcgggtaaat agctgcgccg atggtttcta caaagatcgt
     7381 tatgtttatc ggcactttgc atcggccgcg ctcccgattc cggaagtgct tgacattggg
     7441 gaatttagcg agagcctgac ctattgcatc tcccgccgtg cacagggtgt cacgttgcaa
     7501 gacctgcctg aaaccgaact gcccgctgtt ctgcagccgg tcgcggaggc catggatgcg
     7561 atcgctgcgg ccgatcttag ccagacgagc gggttcggcc cattcggacc gcaaggaatc
     7621 ggtcaataca ctacatggcg tgatttcata tgcgcgattg ctgatcccca tgtgtatcac
     7681 tggcaaactg tgatggacga caccgtcagt gcgtccgtcg cgcaggctct cgatgagctg
     7741 atgctttggg ccgaggactg ccccgaagtc cggcacctcg tgcacgcgga tttcggctcc
     7801 aacaatgtcc tgacggacaa tggccgcata acagcggtca ttgactggag cgaggcgatg
     7861 ttcggggatt cccaatacga ggtcgccaac atcttcttct ggaggccgtg gttggcttgt
     7921 atggagcagc agacgcgcta cttcgagcgg aggcatccgg agcttgcagg atcgccgcgg
     7981 ctccgggcgt atatgctccg cattggtctt gaccaactct atcagagctt ggttgacggc
     8041 aatttcgatg atgcagcttg ggcgcagggt cgatgcgacg caatcgtccg atccggagcc
     8101 gggactgtcg ggcgtacaca aatcgcccgc agaagcgcgg ccgtctggac cgatggctgt
     8161 gtagaagtac tcgccgatag tggaaaccga cgccccagca ctcgtccgag ggcaaaggaa
     8221 tagggtacct ttaagaccaa tgacttacaa ggcagctgta gatcttagcc actttttaaa
     8281 agaaaagggg ggactggaag ggctaattca ctcccaacga agacaagatc tgctttttgc
     8341 ttgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     8401 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     8461 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     8521 ctctagcagt agtagttcat gtcatcttat tattcagtat ttataacttg caaagaaatg
     8581 aatatcagag agtgagagga acttgtttat tgcagcttat aatggttaca aataaagcaa
     8641 tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     8701 caaactcatc aatgtatctt atcatgtctg gctctagcta tcccgcccct aactccgccc
     8761 atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg actaattttt
     8821 tttatttatg cagaggccga ggccgcctcg gcctctgagc tattccagaa gtagtgagga
     8881 ggcttttttg gaggcctagg gacgtaccca attcgcccta tagtgagtcg tattacgcgc
     8941 gctcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
     9001 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
     9061 atcgcccttc ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg
     9121 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc
     9181 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     9241 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     9301 accccaaaaa acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     9361 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     9421 gaacaacact caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt
     9481 cggcctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     9541 tattaacgct tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     9601 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     9661 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     9721 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     9781 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     9841 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     9901 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     9961 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
    10021 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
    10081 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
    10141 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
    10201 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
    10261 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
    10321 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
    10381 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
    10441 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
    10501 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
    10561 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
    10621 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
    10681 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
    10741 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
    10801 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
    10861 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
    10921 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
    10981 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
    11041 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
    11101 acagcgtgag ctatgagaaa gcgccacgct tcccgaagag agaaaggcgg acaggtatcc
    11161 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
    11221 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
    11281 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
    11341 ggccttttgc tggccttttg ctcacatgtt ctttcctgcg ttatcccctg attctgtgga
    11401 taaccgtatt accgcctttg agtgagctga taccgctcgc cgcagccgaa cgaccgagcg
    11461 cagcgagtca gtgagcgagg aagcggaaga gcgcccaata cgcaaaccgc ctctccccgc
    11521 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
    11581 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
    11641 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
    11701 cagctatgac catgattacg ccaagcgcgc aattaaccct cactaaaggg aacaaaagct
    11761 ggagctgcaa gctt
//
