LOCUS       sequence-353156-        2600 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  One-step assembly of custom RVD arrays to construct DdCBE, TALE, 
            etc..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 2600)
  AUTHORS   Guo J, Zhang X, Chen X, Sun H, Dai Y, Wang J, Qian X, Tan L, Lou X, 
            Shen B
  TITLE     Precision modeling of mitochondrial diseases in zebrafish via 
            DdCBE-mediated mtDNA base editing.
  JOURNAL   Cell Discov. 2021 Sep 3;7(1):78. doi: 10.1038/s41421-021-00307-9.
  PUBMED    34480028
REFERENCE   2  (bases 1 to 2600)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Discov."; date: "2021-09-3"; volume: "7(1):78. doi"; pages: " 
            10.1038/s41421-021-00307-9"
COMMENT     Sequence Label: RVD-AC10
FEATURES             Location/Qualifiers
     source          1..2600
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        complement(join(186..191,192..208,209..214))
                     /label=tet promoter
                     /note="E. coli promoter for tetracycline efflux protein 
                     gene"
     rep_origin      complement(341..929)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(421..440)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1334..1350
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1334..1350
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     1355..1374
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        1355..1373
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             complement(join(1548..2600,1..138))
                     /codon_start=1
                     /gene="tet"
                     /product="tetracycline efflux protein"
                     /label=TcR
                     /note="confers resistance to tetracycline"
                     /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASHY
                     GVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAGRI
                     VAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLHAPF
                     LAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIM
                     QLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAII
                     AGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSL
                     AALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST"
     primer_bind     2367..2386
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 gccatagtga ctggcgatgc tgtcggaatg gacgatatcc cgcaagaggc ccggcagtac
       61 cggcataacc aagcctatgc ctacagcatc cagggtgacg gtgccgagga tgacgatgag
      121 cgcattgtta gatttcatac acggtgcctg actgcgttag caatttaact gtgataaact
      181 accgcattaa agctaatcga tgataagctg tcaaacatga gaattaattc atgttctttc
      241 ctgcgttatc ccctgattct gtggataacg caggaaagaa catgtgagca aaaggccagc
      301 aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc
      361 ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat
      421 aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc
      481 cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct
      541 cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg
      601 aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc
      661 cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga
      721 ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa
      781 gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta
      841 gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc
      901 agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg
      961 acgctcagtg gaacgacgcg taactcacgt taagggattt tggtcatggg tggctcgacc
     1021 agatatggtc tcgctctaat attggtggca aacaggctct tgagacggtt cagcgcctcc
     1081 ttccagttct ctgtcaagcc cacggactca ccccagatca agttgtagcg attgctagtc
     1141 atgacggtgg caaacaggct cttgaaaccg tccaacgcct tctaccagtt ctctgtcaag
     1201 cccacggact aaccccagcg caagttgtag cgattgcgtc atgagaccag ctttggtcta
     1261 gaggaggcat gatgatatat ttttatcttg tgcaatgtaa catcagagat tttgagacac
     1321 gggccagagc tgccaggaaa cagctatgac catgtaatac gactcactat aggggatatt
     1381 ttattttctc ccaatcaggc ttgatcccca gtaagtcaaa aaatagctcg acatactgtt
     1441 cttccccgat caagggttgg tttgcgcatt cacagttctc cgcaagaatt gattggctcc
     1501 aattcttgga gtggtgaatc cgttagcgag gtgccgccgg cttccattca ggtcgaggtg
     1561 gcccggctcc atgcaccgcg acgcaacgcg gggaggcaga caaggtatag ggcggcgcct
     1621 acaatccatg ccaacccgtt ccatgtgctc gccgaggcgg cataaatcgc cgtgacgatc
     1681 agcggtccaa tgatcgaagt taggctggta agagccgcga gcgatccttg aagctgtccc
     1741 tgatggtcgt catctacctg cctggacagc atggcctgca acgcgggcat cccgatgccg
     1801 ccggaagcga gaagaatcat aatggggaag gccatccagc ctcgcgtcgc gaacgccagc
     1861 aagacgtagc ccagcgcgtc ggccgccatg ccggcgataa tggcctgctt ctcgccgaaa
     1921 cgtttggtgg cgggaccagt gacgaaggct tgagcgaggg cgtgcaagat tccgaatacc
     1981 gcaagcgaca ggccgatcat cgtcgcgctc cagcgaaagc ggtcctcgcc gaaaatgacc
     2041 cagagcgctg ccggcacctg tcctacgagt tgcatgataa agaagacagt cataagtgcg
     2101 gcgacgatag tcatgccccg cgcccaccgg aaggagctga ctgggttgaa ggctctcaag
     2161 ggcatgggtc ggcgctctcc cttatgcgac tcctgcatta ggaagcagcc cagtagtagg
     2221 ttgaggccgt tgagcaccgc cgccgcaagg aatggtgcat gtaaggagat ggcgcccaac
     2281 agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg
     2341 aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca
     2401 cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agagaatcca caggacgggt
     2461 gtggtcgcca tgatcgcgta gtcgatagtg gctccaagta gcgaagcgag caggactggg
     2521 cggcggccaa agcggtcgga cagtgctccg agaacgggtg cgcatagaaa ttgcatcaac
     2581 gcatatagcg ctagcagcac
//
