LOCUS       sequence-35316-e         926 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: HL 3076 (=HL 716 + pHL 1069)
FEATURES             Location/Qualifiers
     source          1..926
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    230..246
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        304..408
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(627..646)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 tcttctcctt tacgcatgct cgcggtacct ttaatcaggt tgttcaggtt agtgacataa
       61 gtctgatggt gcttgccgta gtgatactcg atggtttccg cagaaatgtg cggtgccaga
      121 gcatctttag catatggtag tgcaggtaat tcgaatgaca ttgctactct cctttattat
      181 taatttgcat acgtacaata gccttattgt gcgtatggtc gacagtatct tgttatccgc
      241 tcacaatgtc aatgttatcc gctcacattt atgacgtcag gtggcacttt tcggggaaat
      301 gtgcgcggaa cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg
      361 agacaataac cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa
      421 catttccgtg tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac
      481 ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac
      541 atcgaactgg atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt
      601 ccaatgatga gcacttttaa agttctgcta tgtggcgcgg tattatcccg tattgacgcc
      661 gggcaagagc aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca
      721 ccagtcacag aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc
      781 ataaccatga gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag
      841 gagctaaccg cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa
      901 ccggagctga atgaagccat accaaa
//
