LOCUS       sequence-353164-       12683 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  EGFP fused to the N-terminus of PARP1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12683)
  AUTHORS   Koczor CA, Saville KM, Andrews JF, Clark J, Fang Q, Li J, 
            Al-Rahahleh RQ, Ibrahim M, McClellan S, Makarov MV, Migaud ME, Sobol
            RW
  TITLE     Temporal dynamics of base excision/single-strand break repair 
            protein complex assembly/disassembly are modulated by the 
            PARP/NAD(+)/SIRT6 axis.
  JOURNAL   Cell Rep. 2021 Nov 2;37(5):109917. doi: 
            10.1016/j.celrep.2021.109917.
  PUBMED    34731617
REFERENCE   2  (bases 1 to 12683)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2021-11-2"; volume: "37(5):109917. doi"; pages: " 
            10.1016/j.celrep.2021.109917"
COMMENT     Sequence Label: pLV-Hygro-EF1A-EGFP-PARP1
FEATURES             Location/Qualifiers
     source          1..12683
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        3..229
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             230..410
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    457..582
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1075..1308
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1493..1537
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1803..1920
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     protein_bind    1938..1958
                     /gene="mutant version of attB"
                     /label=attB4
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     promoter        1959..3137
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          2190..3128
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     3085..3105
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    3137..3161
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      3162..3171
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3168..3170,3171..3173,3174..3884)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3168..3170,3171..3173,3174..3884)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3213..3234)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3474..3493)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3821..3842
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     protein_bind    complement(6930..6954)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    6972..7560
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7025..7045)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7443..7454)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     promoter        7591..8090
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(7626..7649)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     8023..8042
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             8107..9132
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             8107..9132
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     LTR             9204..9437
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    9509..9630
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9546..9565)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9600..9619
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      9670..9805
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(9825..9844)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(9826..9844)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(9854..9871)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(9854..9870)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9863..9885)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      10012..10467
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(10099..10118)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     10309..10330
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        10493..10597
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(10598..10666,10667..11458)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(10816..10835)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      11629..12217
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     12118..12137
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     12371..12388
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    12505..12526
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(12541..12546,12547..12564,12565..12571)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12579..12595
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     12584..12606
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     12603..12619
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     12603..12619
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     12638..12658
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        12640..12658
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
ORIGIN
        1 aatgtagtct tatgcaatac tcttgtagtc ttgcaacatg gtaacgatga gttagcaaca
       61 tgccttacaa ggagagaaaa agcaccgtgc atgccgattg gtggaagtaa ggtggtacga
      121 tcgtgcctta ttaggaaggc aacagacggg tctgacatgg attggacgaa ccactgaatt
      181 gccgcattgc agagatattg tatttaagtg cctagctcga tacataaacg ggtctctctg
      241 gttagaccag atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc
      301 tcaataaagc ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg
      361 taactagaga tccctcagac ccttttagtc agtgtggaaa atctctagca gtggcgcccg
      421 aacagggact tgaaagcgaa agggaaacca gaggagctct ctcgacgcag gactcggctt
      481 gctgaagcgc gcacggcaag aggcgagggg cggcgactgg tgagtacgcc aaaaattttg
      541 actagcggag gctagaagga gagagatggg tgcgagagcg tcagtattaa gcgggggaga
      601 attagatcgc gatgggaaaa aattcggtta aggccagggg gaaagaaaaa atataaatta
      661 aaacatatag tatgggcaag cagggagcta gaacgattcg cagttaatcc tggcctgtta
      721 gaaacatcag aaggctgtag acaaatactg ggacagctac aaccatccct tcagacagga
      781 tcagaagaac ttagatcatt atataataca gtagcaaccc tctattgtgt gcatcaaagg
      841 atagagataa aagacaccaa ggaagcttta gacaagatag aggaagagca aaacaaaagt
      901 aagaccaccg cacagcaagc ggccgctgat cttcagacct ggaggaggag atatgaggga
      961 caattggaga agtgaattat ataaatataa agtagtaaaa attgaaccat taggagtagc
     1021 acccaccaag gcaaagagaa gagtggtgca gagagaaaaa agagcagtgg gaataggagc
     1081 tttgttcctt gggttcttgg gagcagcagg aagcactatg ggcgcagcgt caatgacgct
     1141 gacggtacag gccagacaat tattgtctgg tatagtgcag cagcagaaca atttgctgag
     1201 ggctattgag gcgcaacagc atctgttgca actcacagtc tggggcatca agcagctcca
     1261 ggcaagaatc ctggctgtgg aaagatacct aaaggatcaa cagctcctgg ggatttgggg
     1321 ttgctctgga aaactcattt gcaccactgc tgtgccttgg aatgctagtt ggagtaataa
     1381 atctctggaa cagatttgga atcacacgac ctggatggag tgggacagag aaattaacaa
     1441 ttacacaagc ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga
     1501 acaagaatta ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa
     1561 ttggctgtgg tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat
     1621 agtttttgct gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt
     1681 tcagacccac ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg
     1741 tggagagaga gacagagaca gatccattcg attagtgaac ggatctcgac ggtatcgcta
     1801 gcttttaaaa gaaaaggggg gattgggggg tacagtgcag gggaaagaat agtagacata
     1861 atagcaacag acatacaaac taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt
     1921 actagtgatt atcggatcaa ctttgtatag aaaagttggg ctccggtgcc cgtcagtggg
     1981 cagagcgcac atcgcccaca gtccccgaga agttgggggg aggggtcggc aattgaaccg
     2041 gtgcctagag aaggtggcgc ggggtaaact gggaaagtga tgtcgtgtac tggctccgcc
     2101 tttttcccga gggtggggga gaaccgtata taagtgcagt agtcgccgtg aacgttcttt
     2161 ttcgcaacgg gtttgccgcc agaacacagg taagtgccgt gtgtggttcc cgcgggcctg
     2221 gcctctttac gggttatggc ccttgcgtgc cttgaattac ttccacctgg ctgcagtacg
     2281 tgattcttga tcccgagctt cgggttggaa gtgggtggga gagttcgagg ccttgcgctt
     2341 aaggagcccc ttcgcctcgt gcttgagttg aggcctggcc tgggcgctgg ggccgccgcg
     2401 tgcgaatctg gtggcacctt cgcgcctgtc tcgctgcttt cgataagtct ctagccattt
     2461 aaaatttttg atgacctgct gcgacgcttt ttttctggca agatagtctt gtaaatgcgg
     2521 gccaagatct gcacactggt atttcggttt ttggggccgc gggcggcgac ggggcccgtg
     2581 cgtcccagcg cacatgttcg gcgaggcggg gcctgcgagc gcggccaccg agaatcggac
     2641 gggggtagtc tcaagctggc cggcctgctc tggtgcctgg tctcgcgccg ccgtgtatcg
     2701 ccccgccctg ggcggcaagg ctggcccggt cggcaccagt tgcgtgagcg gaaagatggc
     2761 cgcttcccgg ccctgctgca gggagctcaa aatggaggac gcggcgctcg ggagagcggg
     2821 cgggtgagtc acccacacaa aggaaaaggg cctttccgtc ctcagccgtc gcttcatgtg
     2881 actccacgga gtaccgggcg ccgtccaggc acctcgatta gttctcgagc ttttggagta
     2941 cgtcgtcttt aggttggggg gaggggtttt atgcgatgga gtttccccac actgagtggg
     3001 tggagactga agttaggcca gcttggcact tgatgtaatt ctccttggaa tttgcccttt
     3061 ttgagtttgg atcttggttc attctcaagc ctcagacagt ggttcaaagt ttttttcttc
     3121 catttcaggt gtcgtgacaa gtttgtacaa aaaagcaggc tgccaccatg gtgagcaagg
     3181 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     3241 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     3301 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
     3361 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
     3421 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     3481 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     3541 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     3601 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
     3661 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
     3721 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
     3781 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     3841 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caagatggcg gagtcttcgg
     3901 ataagctcta tcgagtcgag tacgccaaga gcgggcgcgc ctcttgcaag aaatgcagcg
     3961 agagcatccc caaggactcg ctccggatgg ccatcatggt gcagtcgccc atgtttgatg
     4021 gaaaagtccc acactggtac cacttctcct gcttctggaa ggtgggccac tccatccggc
     4081 accctgacgt tgaggtggat gggttctctg agcttcggtg ggatgaccag cagaaagtca
     4141 agaagacagc ggaagctgga ggagtgacag gcaaaggcca ggatggaatt ggtagcaagg
     4201 cagagaagac tctgggtgac tttgcagcag agtatgccaa gtccaacaga agtacgtgca
     4261 aggggtgtat ggagaagata gaaaagggcc aggtgcgcct gtccaagaag atggtggacc
     4321 cggagaagcc acagctaggc atgattgacc gctggtacca tccaggctgc tttgtcaaga
     4381 acagggagga gctgggtttc cggcccgagt acagtgcgag tcagctcaag ggcttcagcc
     4441 tccttgctac agaggataaa gaagccctga agaagcagct cccaggagtc aagagtgaag
     4501 gaaagagaaa aggcgatgag gtggatggag tggatgaagt ggcgaagaag aaatctaaaa
     4561 aagaaaaaga caaggatagt aagcttgaaa aagccctaaa ggctcagaac gacctgatct
     4621 ggaacatcaa ggacgagcta aagaaagtgt gttcaactaa tgacctgaag gagctactca
     4681 tcttcaacaa gcagcaagtg ccttctgggg agtcggcgat cttggaccga gtagctgatg
     4741 gcatggtgtt cggtgccctc cttccctgcg aggaatgctc gggtcagctg gtcttcaaga
     4801 gcgatgccta ttactgcact ggggacgtca ctgcctggac caagtgtatg gtcaagacac
     4861 agacacccaa ccggaaggag tgggtaaccc caaaggaatt ccgagaaatc tcttacctca
     4921 agaaattgaa ggttaaaaaa caggaccgta tattcccccc agaaaccagc gcctccgtgg
     4981 cggccacgcc tccgccctcc acagcctcgg ctcctgctgc tgtgaactcc tctgcttcag
     5041 cagataagcc attatccaac atgaagatcc tgactctcgg gaagctgtcc cggaacaagg
     5101 atgaagtgaa ggccatgatt gagaaactcg gggggaagtt gacggggacg gccaacaagg
     5161 cttccctgtg catcagcacc aaaaaggagg tggaaaagat gaataagaag atggaggaag
     5221 taaaggaagc caacatccga gttgtgtctg aggacttcct ccaggacgtc tccgcctcca
     5281 ccaagagcct tcaggagttg ttcttagcgc acatcttgtc cccttggggg gcagaggtga
     5341 aggcagagcc tgttgaagtt gtggccccaa gagggaagtc aggggctgcg ctctccaaaa
     5401 aaagcaaggg ccaggtcaag gaggaaggta tcaacaaatc tgaaaagaga atgaaattaa
     5461 ctcttaaagg aggagcagct gtggatcctg attctggact ggaacactct gcgcatgtcc
     5521 tggagaaagg tgggaaggtc ttcagtgcca cccttggcct ggtggacatc gttaaaggaa
     5581 ccaactccta ctacaagctg cagcttctgg aggacgacaa ggaaaacagg tattggatat
     5641 tcaggtcctg gggccgtgtg ggtacggtga tcggtagcaa caaactggaa cagatgccgt
     5701 ccaaggagga tgccattgag cacttcatga aattatatga agaaaaaacc gggaacgctt
     5761 ggcactccaa aaatttcacg aagtatccca aaaagttcta ccccctggag attgactatg
     5821 gccaggatga agaggcagtg aagaagctga cagtaaatcc tggcaccaag tccaagctcc
     5881 ccaagccagt tcaggacctc atcaagatga tctttgatgt ggaaagtatg aagaaagcca
     5941 tggtggagta tgagatcgac cttcagaaga tgcccttggg gaagctgagc aaaaggcaga
     6001 tccaggccgc atactccatc ctcagtgagg tccagcaggc ggtgtctcag ggcagcagcg
     6061 actctcagat cctggatctc tcaaatcgct tttacaccct gatcccccac gactttggga
     6121 tgaagaagcc tccgctcctg aacaatgcag acagtgtgca ggccaaggtg gaaatgcttg
     6181 acaacctgct ggacatcgag gtggcctaca gtctgctcag gggagggtct gatgatagca
     6241 gcaaggatcc catcgatgtc aactatgaga agctcaaaac tgacattaag gtggttgaca
     6301 gagattctga agaagccgag atcatcagga agtatgttaa gaacactcat gcaaccacac
     6361 acaatgcgta tgacttggaa gtcatcgata tctttaagat agagcgtgaa ggcgaatgcc
     6421 agcgttacaa gccctttaag cagcttcata accgaagatt gctgtggcac gggtccagga
     6481 ccaccaactt tgctgggatc ctgtcccagg gtcttcggat agccccgcct gaagcgcccg
     6541 tgacaggcta catgtttggt aaagggatct atttcgctga catggtctcc aagagtgcca
     6601 actactgcca tacgtctcag ggagacccaa taggcttaat cctgttggga gaagttgccc
     6661 ttggaaacat gtatgaactg aagcacgctt cacatatcag caagttaccc aagggcaagc
     6721 acagtgtcaa aggtttgggc aaaactaccc ctgatccttc agctaacatt agtctggatg
     6781 gtgtagacgt tcctcttggg accgggattt catctggtgt gaatgacacc tctctactat
     6841 ataacgagta cattgtctat gatattgctc aggtaaatct gaagtatctg ctgaaactga
     6901 aattcaattt taagacctcc ctgtggtaaa cccagctttc ttgtacaaag tggtgataat
     6961 cgaattccga taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     7021 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     7081 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     7141 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     7201 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     7261 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     7321 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaagctg acgtcctttc
     7381 catggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     7441 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     7501 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     7561 atcgggaatt cccgcggttc gaattctacc gggtagggga ggcgcttttc ccaaggcagt
     7621 ctggagcatg cgctttagca gccccgctgg gcacttggcg ctacacaagt ggcctctggc
     7681 ctcgcacaca ttccacatcc accggtaggc gccaaccggc tccgttcttt ggtggcccct
     7741 tcgcgccacc ttctactcct cccctagtca ggaagttccc ccccgccccg cagctcgcgt
     7801 cgtgcaggac gtgacaaatg gaagtagcac gtctcactag tctcgtgcag atggacagca
     7861 ccgctgagca atggaagcgg gtaggccttt ggggcagcgg ccaatagcag ctttgctcct
     7921 tcgctttctg ggctcagagg ctgggaaggg gtgggtccgg gggcgggctc aggggcgggc
     7981 tcaggggcgg ggcgggcgcc cgaaggtcct ccggaggccc ggcattctgc acgcttcaaa
     8041 agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acctcacgtg
     8101 gccaccatga aaaagcctga actcaccgcg acgtctgtcg agaagtttct gatcgaaaag
     8161 ttcgacagcg tctccgacct gatgcagctc tcggagggcg aagaatctcg tgctttcagc
     8221 ttcgatgtag gagggcgtgg atatgtcctg cgggtaaata gctgcgccga tggtttctac
     8281 aaagatcgtt atgtttatcg gcactttgca tcggccgcgc tcccgattcc ggaagtgctt
     8341 gacattgggg aatttagcga gagcctgacc tattgcatct cccgccgtgc acagggtgtc
     8401 acgttgcaag acctgcctga aaccgaactg cccgctgttc tgcagccggt cgcggaggcc
     8461 atggatgcga tcgctgcggc cgatcttagc cagacgagcg ggttcggccc attcggaccg
     8521 caaggaatcg gtcaatacac tacatggcgt gatttcatat gcgcgattgc tgatccccat
     8581 gtgtatcact ggcaaactgt gatggacgac accgtcagtg cgtccgtcgc gcaggctctc
     8641 gatgagctga tgctttgggc cgaggactgc cccgaagtcc ggcacctcgt gcacgcggat
     8701 ttcggctcca acaatgtcct gacggacaat ggccgcataa cagcggtcat tgactggagc
     8761 gaggcgatgt tcggggattc ccaatacgag gtcgccaaca tcttcttctg gaggccgtgg
     8821 ttggcttgta tggagcagca gacgcgctac ttcgagcgga ggcatccgga gcttgcagga
     8881 tcgccgcggc tccgggcgta tatgctccgc attggtcttg accaactcta tcagagcttg
     8941 gttgacggca atttcgatga tgcagcttgg gcgcagggtc gatgcgacgc aatcgtccga
     9001 tccggagccg ggactgtcgg gcgtacacaa atcgcccgca gaagcgcggc cgtctggacc
     9061 gatggctgtg tagaagtact cgccgatagt ggaaaccgac gccccagcac tcgtccgagg
     9121 gcaaaggaat agggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     9181 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatct
     9241 gctttttgct tgtactgggt ctctctggtt agaccagatc tgagcctggg agctctctgg
     9301 ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt
     9361 gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt
     9421 gtggaaaatc tctagcagta gtagttcatg tcatcttatt attcagtatt tataacttgc
     9481 aaagaaatga atatcagaga gtgagaggaa cttgtttatt gcagcttata atggttacaa
     9541 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
     9601 tggtttgtcc aaactcatca atgtatctta tcatgtctgg ctctagctat cccgccccta
     9661 actccgccca tcccgcccct aactccgccc agttccgccc attctccgcc ccatggctga
     9721 ctaatttttt ttatttatgc agaggccgag gccgcctcgg cctctgagct attccagaag
     9781 tagtgaggag gcttttttgg aggcctaggg acgtacccaa ttcgccctat agtgagtcgt
     9841 attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac cctggcgtta
     9901 cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat agcgaagagg
     9961 cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg gacgcgccct
    10021 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
    10081 ccagcgccct agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
    10141 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
    10201 ggcacctcga ccccaaaaaa cttgattagg gtgatggttc acgtagtggg ccatcgccct
    10261 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
    10321 tccaaactgg aacaacactc aaccctatct cggtctattc ttttgattta taagggattt
    10381 tgccgatttc ggcctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
    10441 ttaacaaaat attaacgctt acaatttagg tggcactttt cggggaaatg tgcgcggaac
    10501 ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga gacaataacc
    10561 ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac atttccgtgt
    10621 cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc cagaaacgct
    10681 ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca tcgaactgga
    10741 tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc caatgatgag
    10801 cacttttaaa gttctgctat gtggcgcggt attatcccgt attgacgccg ggcaagagca
    10861 actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac cagtcacaga
    10921 aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca taaccatgag
    10981 tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg agctaaccgc
    11041 ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac cggagctgaa
    11101 tgaagccata ccaaacgacg agcgtgacac cacgatgcct gtagcaatgg caacaacgtt
    11161 gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat taatagactg
    11221 gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg ctggctggtt
    11281 tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg cagcactggg
    11341 gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc aggcaactat
    11401 ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc attggtaact
    11461 gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt tttaatttaa
    11521 aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt aacgtgagtt
    11581 ttcgttccac tgagcgtcag accccgtaga aaagatcaaa ggatcttctt gagatccttt
    11641 ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg
    11701 tttgccggat caagagctac caactctttt tccgaaggta actggcttca gcagagcgca
    11761 gataccaaat actgttcttc tagtgtagcc gtagttaggc caccacttca agaactctgt
    11821 agcaccgcct acatacctcg ctctgctaat cctgttacca gtggctgctg ccagtggcga
    11881 taagtcgtgt cttaccgggt tggactcaag acgatagtta ccggataagg cgcagcggtc
    11941 gggctgaacg gggggttcgt gcacacagcc cagcttggag cgaacgacct acaccgaact
    12001 gagataccta cagcgtgagc tatgagaaag cgccacgctt cccgaagaga gaaaggcgga
    12061 caggtatccg gtaagcggca gggtcggaac aggagagcgc acgagggagc ttccaggggg
    12121 aaacgcctgg tatctttata gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt
    12181 tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac gccagcaacg cggccttttt
    12241 acggttcctg gccttttgct ggccttttgc tcacatgttc tttcctgcgt tatcccctga
    12301 ttctgtggat aaccgtatta ccgcctttga gtgagctgat accgctcgcc gcagccgaac
    12361 gaccgagcgc agcgagtcag tgagcgagga agcggaagag cgcccaatac gcaaaccgcc
    12421 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
    12481 agcgggcagt gagcgcaacg caattaatgt gagttagctc actcattagg caccccaggc
    12541 tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt gtgagcggat aacaatttca
    12601 cacaggaaac agctatgacc atgattacgc caagcgcgca attaaccctc actaaaggga
    12661 acaaaagctg gagctgcaag ctt
//
