LOCUS       sequence-353179-        9853 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9853)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9853)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2002
FEATURES             Location/Qualifiers
     source          1..9853
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    2791..3379
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2844..2864)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3262..3273)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3451..3684
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3762..3883
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3799..3818)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3853..3872
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3923..4058
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4078..4097)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4079..4097)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4107..4124)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4107..4123)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4116..4138)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4265..4720
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4352..4371)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4562..4583
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4746..4850
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4851..4919,4920..5711)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5069..5088)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5882..6470
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6371..6390
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6624..6641
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6758..6779
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6794..6799,6800..6817,6818..6824)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6832..6848
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6837..6859
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6856..6872
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6856..6872
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6891..6911
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6893..6911
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6939..7165
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7166..7346
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7393..7518
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8011..8244
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8429..8473
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8704..8914
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8726..8922
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8773..8908
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8954..9556
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8954..8973)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     9450..9470
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    9599..9716
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgac
     1201 cctgggcagc tgctgctgcg agatcatgtc ctccgagagc tccccggccg cgctgtccga
     1261 ggccgacgca gacatagacg tggtgggcgg cggcagcggc gggggggagc tcccagctcg
     1321 ctccgggccc cgcgcccccc gggacgtgct cccccacggc cacgagcctc ccgcggagga
     1381 agccgaggca gacttagccg aggacgagga ggagtctggt ggctgctcgg acggcgagcc
     1441 ccgcgctctg gcgtcccggg gggcggcggc cgcagcgggg agcccggggc caggcgccgc
     1501 ggcggcccgc ggcgcagcgg ggcccgggcc gggaccgccg tcggggggcg cggcgacgcg
     1561 gagcccgctg gtgaagccgc cctactcgta catcgcgctc atcaccatgg ccatcctgca
     1621 gagccccaag aagcggctga cgttgagcga gatctgcgag ttcatcagcg gccgcttccc
     1681 ctactaccgg gagaagttcc ccgcctggca gaacagcatc cgccacaacc tctctctcaa
     1741 cgactgcttc gtcaagatcc cccgcgagcc gggcaacccg ggcaagggca actactggac
     1801 gctggacccg gagtcggccg acatgttcga caacggcagc ttcctgcggc gtcgcaagcg
     1861 cttcaagcgg cagcccctgc cgccgccgca cccacacccg caccctcacc cggagctgct
     1921 gctgcgtggc ggggccgcgg cggcggggga tcccggcgct ttcctgcccg gcttcgctgc
     1981 ctacggcgcc tacggctacg gctacgggct ggctctcccg gcctacggcg cacccccgcc
     2041 ggggccggcc ccgcatccgc acccgcaccc gcacgccttc gctttcgccg cggcagccgc
     2101 cgccgctcct tgccagctgt cggtaccccc aggccgcgcc gccgcgcctc cacccggacc
     2161 tccgacggcc tcggtgttcg caggcgcggg atcggcccca gctcctgcgc ctgcctcagg
     2221 ctcgggcccg ggcccgggcc ccgcaggcct gcccgccttc ctgggcgcgg agctgggctg
     2281 cgccaaagcc ttctacgcgg cgtccctgag tcctcccgca gccggcaccg cggcgggtct
     2341 gcccaccgca cttctgcgcc agggcctcaa gacggacgcg ggcggtggtg caggcggcgg
     2401 gggcgccggg gcagggcaga ggccttcctt ctctatagac cacatcatgg gccacggtgg
     2461 cggcggggca gcacccccgg gcgccggcga gggctctccg ggaccgccat tcgcggcagc
     2521 cgcgggtcct gggggccaag cccaggtctt ggccatgctg actgctccgg ccctggctcc
     2581 cgttgctggc cacattcgcc tctcgcatcc cggggacgcg ctgctgtcct cagggtcccg
     2641 gtttgccagc aaagtcgccg gccttagtgg ctgccacttc tagtaatgaa ctagtccgta
     2701 acttgaaagt atttcgattt cttggcttta tatatcttgt ggaaaggacg actcagtcac
     2761 acgcgaatca cgtttacgcg ttaagtcgac aatcaacctc tggattacaa aatttgtgaa
     2821 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     2881 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     2941 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     3001 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     3061 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     3121 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     3181 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     3241 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     3301 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     3361 ctttgggccg cctccccgcg tcgactttaa gaccaatgac ttacaaggca gctgtagatc
     3421 ttagccactt tttaaaagaa aaggggggac tggaagggct aattcactcc caacgaagac
     3481 aagatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
     3541 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
     3601 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
     3661 agtcagtgtg gaaaatctct agcagtacgt atagtagttc atgtcatctt attattcagt
     3721 atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt attgcagctt
     3781 ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac
     3841 tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tggctctagc
     3901 tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     3961 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     4021 gctattccag aagtagtgag gaggcttttt tggaggccta gggacgtacc caattcgccc
     4081 tatagtgagt cgtattacgc gcgctcactg gccgtcgttt tacaacgtcg tgactgggaa
     4141 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
     4201 aatagcgaag aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa
     4261 tgggacgcgc cctgtagcgg cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg
     4321 accgctacac ttgccagcgc cctagcgccc gctcctttcg ctttcttccc ttcctttctc
     4381 gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg ggctcccttt agggttccga
     4441 tttagtgctt tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt
     4501 gggccatcgc cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat
     4561 agtggactct tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat
     4621 ttataaggga ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa
     4681 tttaacgcga attttaacaa aatattaacg cttacaattt aggtggcact tttcggggaa
     4741 atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca
     4801 tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc
     4861 aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc
     4921 acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt
     4981 acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt
     5041 ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtattgacg
     5101 ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg gttgagtact
     5161 caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta tgcagtgctg
     5221 ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc ggaggaccga
     5281 aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt gatcgttggg
     5341 aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg cctgtagcaa
     5401 tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct tcccggcaac
     5461 aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc tcggcccttc
     5521 cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct cgcggtatca
     5581 ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac acgacgggga
     5641 gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc tcactgatta
     5701 agcattggta actgtcagac caagtttact catatatact ttagattgat ttaaaacttc
     5761 atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     5821 cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     5881 cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     5941 cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     6001 tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta ggccaccact
     6061 tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     6121 ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     6181 aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     6241 cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     6301 ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     6361 agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     6421 ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     6481 acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     6541 cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc
     6601 gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgcccaa
     6661 tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg cacgacaggt
     6721 ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag ctcactcatt
     6781 aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga attgtgagcg
     6841 gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc
     6901 ctcactaaag ggaacaaaag ctggagctgc aagcttaatg tagtcttatg caatactctt
     6961 gtagtcttgc aacatggtaa cgatgagtta gcaacatgcc ttacaaggag agaaaaagca
     7021 ccgtgcatgc cgattggtgg aagtaaggtg gtacgatcgt gccttattag gaaggcaaca
     7081 gacgggtctg acatggattg gacgaaccac tgaattgccg cattgcagag atattgtatt
     7141 taagtgccta gctcgataca taaacgggtc tctctggtta gaccagatct gagcctggga
     7201 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     7261 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     7321 ttagtcagtg tggaaaatct ctagcagtgg cgcccgaaca gggacttgaa agcgaaaggg
     7381 aaaccagagg agctctctcg acgcaggact cggcttgctg aagcgcgcac ggcaagaggc
     7441 gaggggcggc gactggtgag tacgccaaaa attttgacta gcggaggcta gaaggagaga
     7501 gatgggtgcg agagcgtcag tattaagcgg gggagaatta gatcgcgatg ggaaaaaatt
     7561 cggttaaggc cagggggaaa gaaaaaatat aaattaaaac atatagtatg ggcaagcagg
     7621 gagctagaac gattcgcagt taatcctggc ctgttagaaa catcagaagg ctgtagacaa
     7681 atactgggac agctacaacc atcccttcag acaggatcag aagaacttag atcattatat
     7741 aatacagtag caaccctcta ttgtgtgcat caaaggatag agataaaaga caccaaggaa
     7801 gctttagaca agatagagga agagcaaaac aaaagtaaga ccaccgcaca gcaagcggcc
     7861 gctgatcttc agacctggag gaggagatat gagggacaat tggagaagtg aattatataa
     7921 atataaagta gtaaaaattg aaccattagg agtagcaccc accaaggcaa agagaagagt
     7981 ggtgcagaga gaaaaaagag cagtgggaat aggagctttg ttccttgggt tcttgggagc
     8041 agcaggaagc actatgggcg cagcgtcaat gacgctgacg gtacaggcca gacaattatt
     8101 gtctggtata gtgcagcagc agaacaattt gctgagggct attgaggcgc aacagcatct
     8161 gttgcaactc acagtctggg gcatcaagca gctccaggca agaatcctgg ctgtggaaag
     8221 atacctaaag gatcaacagc tcctggggat ttggggttgc tctggaaaac tcatttgcac
     8281 cactgctgtg ccttggaatg ctagttggag taataaatct ctggaacaga tttggaatca
     8341 cacgacctgg atggagtggg acagagaaat taacaattac acaagcttaa tacactcctt
     8401 aattgaagaa tcgcaaaacc agcaagaaaa gaatgaacaa gaattattgg aattagataa
     8461 atgggcaagt ttgtggaatt ggtttaacat aacaaattgg ctgtggtata taaaattatt
     8521 cataatgata gtaggaggct tggtaggttt aagaatagtt tttgctgtac tttctatagt
     8581 gaatagagtt aggcagggat attcaccatt atcgtttcag acccacctcc caaccccgag
     8641 gggacccatg cattgcatct caattagtca gcaaccaggt gtggaaagtc cccaggctcc
     8701 ccagcaggca gaagtatgca aagcatgcgt ctcaattagt cagcaaccat agtcccgccc
     8761 ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc gccccatggc
     8821 tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga gctattccag
     8881 aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagctttc tagaggtacc
     8941 accggagctt accatgaccg agtacaagcc cacggtgcgc ctcgccaccc gcgacgacgt
     9001 ccccagggcc gtacgcaccc tcgccgccgc gttcgccgac taccccgcca cgcgccacac
     9061 cgtcgatccg gaccgccaca tcgagcgggt caccgagctg caagaactct tcctcacgcg
     9121 cgtcgggctc gacatcggca aggtgtgggt cgcggacgac ggcgccgccg tggcggtctg
     9181 gaccacgccg gagagcgtcg aagcgggggc ggtgttcgcc gagatcggcc cgcgcatggc
     9241 cgagttgagc ggttcccggc tggccgcgca gcaacagatg gaaggcctcc tggcgccgca
     9301 ccggcccaag gagcccgcgt ggttcctggc caccgtcggc gtctcgcccg accaccaggg
     9361 caagggtctg ggcagcgccg tcgtgctccc cggagtggag gcggccgagc gcgccggggt
     9421 gcccgccttc ctggagacct ccgcgccccg caacctcccc ttctacgagc ggctcggctt
     9481 caccgtcacc gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa
     9541 gcccggtgcc agttaacgaa ttctagatct tgagacaaat ggcagtattc atccacaatt
     9601 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     9661 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg
     9721 tttattacag ggacagcaga gatccacttt ggcgccggct cgaggcctgc aggtgcaaag
     9781 atggataaag ttttaaacag agaggaatct ttgcagctaa tggaccttct aggtcttgaa
     9841 aggagtggga att
//
