LOCUS       sequence-35318-e         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: HL 2839 (=HL 2752 + pHL 720)
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    142..160
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    167..185
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      complement(252..279)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(371..457)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     610..627
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     primer_bind     complement(713..733)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 ctcgcctcgc cctcgatctc gaactcgtgg ccgttcacgg agccctccat gtgcaccttg
       61 aagcgcatga actccttgat gatggccatg ttatcctcct cgcccttgct caccattttt
      121 tttcctcctt acccgggagt atctctatca ctgataggga tgtcaatctc tatcactgat
      181 agggaggatc ctgagcggat acatatttga atgtatttag aaaaataaac aaaagagttt
      241 gtagaaacgc aaaaaggcca tccgtcagga tggccttctg cttaatttga tgcctggcag
      301 tttatggcgg gcgtcctgcc cgccaccctc cgggccgttg cttcgcaacg ttcaaatccg
      361 ctcccggcgg atttgtccta ctcaggagag cgttcaccga caaacaacag ataaaacgaa
      421 aggcccagtc tttcgactga gcctttcgtt ttatttgatg cctggcagtt ccctactctc
      481 gcatggggag accccacact accatcggcg ctacggcgtt tcacttctga gttcggcatg
      541 gggtcaggtg ggaccaccgc gctactgccg ccaggcaaat tctgttttat cagaccgctt
      601 ctgcgttctg atttaatctg tatcaggctg aaaatcttct ctcatccgcc aaaacagcca
      661 agctagcttt tatttgtaga gctcatccat gccatgtgta atcccagcag ctgttacaaa
      721 ctcaagaagg accatgtggt ctctcttttc gttgggatct ttcgaaaggg cagattgtgt
      781 ggacaggtaa tggttgtctg gtaaaaggac agggccatcg ccaattggag tattttgttg
      841 ataatggtct gctagttgaa cgcttccatc ttcaatgttg tgtctaattt tgaagttaac
      901 tttgattcca ttcttttgtt
//
