LOCUS       sequence-353188-       14299 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14299)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 14299)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2039
FEATURES             Location/Qualifiers
     source          1..14299
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    7237..7825
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7290..7310)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7708..7719)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7897..8130
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    8208..8329
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8245..8264)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8299..8318
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      8369..8504
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(8524..8543)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(8525..8543)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(8553..8570)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(8553..8569)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8562..8584)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      8711..9166
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8798..8817)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9008..9029
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        9192..9296
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9297..9365,9366..10157)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9515..9534)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10328..10916
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     10817..10836
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11070..11087
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11204..11225
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11240..11245,11246..11263,11264..11270)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11278..11294
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11283..11305
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11302..11318
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11302..11318
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     11337..11357
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        11339..11357
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        11385..11611
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             11612..11792
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    11839..11964
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    12457..12690
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             12875..12919
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        13150..13360
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        13172..13368
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      13219..13354
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             13400..14002
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(13400..13419)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     13896..13916
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    14045..14162
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgcc
     1201 tggggagcag atggacccta ctggaagtca gttggattca gatttctctc agcaagatac
     1261 tccttgcctg ataattgaag attctcagcc tgaaagccag gttctagagg atgattctgg
     1321 ttctcacttc agtatgctat ctcgacacct tcctaatctc cagacgcaca aagaaaatcc
     1381 tgtgttggat gttgtgtcca atcctgaaca aacagctgga gaagaacgag gagacggtaa
     1441 tagtgggttc aatgaacatt tgaaagaaaa caaggttgca gaccctgtgg attcttctaa
     1501 cttggacaca tgtggttcca tcagtcaggt cattgagcag ttacctcagc caaacaggac
     1561 aagcagtgtt ctgggaatgt cagtggaatc tgctcctgct gtggaggaag agaagggaga
     1621 agagttggaa cagaaggaga aagagaagga agaagatact tcaggcaata ctacacattc
     1681 ccttggtgct gaagatactg cctcatcaca gttgggtttt ggggttctgg aactctccca
     1741 gagccaggat gttgaggaaa atactgtgcc atatgaagtg gacaaagagc agctacaatc
     1801 agtaaccacc aactctggtt ataccaggct gtctgatgtg gatgctaata ctgcaattaa
     1861 gcatgaagaa cagtccaacg aagatatccc catagcagaa cagtccagca aggacatccc
     1921 tgtgacagca cagcccagta aggatgtaca tgttgtaaaa gagcaaaatc caccacctgc
     1981 aaggtcagag gacatgcctt ttagccccaa agcatctgtt gctgctatgg aagcaaaaga
     2041 acagttgtct gcacaagaac ttatggaaag tggactgcag attcagaagt caccagagcc
     2101 tgaggttttg tcaactcagg aagacttgtt tgaccagagc aataaaacag tatcttctga
     2161 tggttgctct actccttcaa gggaggaagg tgggtgttct ttggcttcca ctcctgccac
     2221 cactctgcat ctcctgcagc tctctggtca gaggtccctt gttcaggaca gtctttccac
     2281 gaattcttca gatcttgttg ctccttctcc tgatgctttc cgatctactc cttttatcgt
     2341 tcctagcagt cccacagagc aagaagggag acaagataag ccaatggaca cgtcagtgtt
     2401 atctgaagaa ggaggagagc cttttcagaa gaaacttcaa agtggtgaac cagtggagtt
     2461 agaaaacccc cctctcctgc ctgagtccac tgtatcacca caagcctcaa caccaatatc
     2521 tcagagcaca ccagtcttcc ctcctgggtc acttcctatc ccatcccagc ctcagttttc
     2581 tcatgacatt tttattcctt ccccaagtct ggaagaacaa tcaaatgatg ggaagaaaga
     2641 tggagatatg catagttcat ctttgacagt tgagtgttct aaaacttcag agattgaacc
     2701 aaagaattcc cctgaggatc ttgggctatc tttgacaggg gattcttgca agttgatgct
     2761 ttctacaagt gaatatagtc agtccccaaa gatggagagc ttgagttctc acagaattga
     2821 tgaagatgga gaaaacacac agattgagga tacggaaccc atgtctccag ttctcaattc
     2881 taaatttgtt cctgctgaaa atgatagtat cctgatgaat ccagcacagg atggtgaagt
     2941 acaactgagt cagaatgatg acaaaacaaa gggagatgat acagacacca gggatgacat
     3001 tagtatttta gccactggtt gcaagggcag agaagaaacg gtagcagaag atgtttgtat
     3061 tgatctcact tgtgattcgg ggagtcaggc agttccgtca ccagctactc gatctgaggc
     3121 actttctagt gtgttagatc aggaggaagc tatggaaatt aaagaacacc atccagagga
     3181 ggggtcttca gggtctgagg tggaagaaat ccctgagaca ccttgtgaaa gtcaaggaga
     3241 ggaactcaaa gaagaaaata tggagagtgt tccgttgcac ctttctctga ctgaaactca
     3301 gtcccaaggg ttgtgtcttc aaaaggaaat gccaaaaaaa gaatgctcag aagctatgga
     3361 agttgaaacc agtgtgatta gtattgattc ccctcaaaag ttggcaatac ttgaccaaga
     3421 attggaacat aaggaacagg aagcttggga agaagctact tcagaggact ccagtgttgt
     3481 cattgtagat gtgaaagagc catctcccag agttgatgtt tcttgtgaac ctttggaggg
     3541 agtggagaag tgctcagatt cccagtcatg ggaggatatt gctccagaaa tagaaccatg
     3601 tgctgagaat agattagaca ccaaggaaga aaagagtgta gaatatgaag gagatctgaa
     3661 atcagggact gcagaaacag aacctgtaga gcaagattct tcacagcctt ccttaccttt
     3721 agtgagagca gatgatcctt taagacttga ccaggagttg cagcagcccc aaactcagga
     3781 gaaaacaagt aattcattaa cagaagactc aaaaatggct aatgcaaagc agctaagctc
     3841 agatgcagag gcccagaagc tggggaagcc ctctgcccat gcctcacaaa gcttctgtga
     3901 aagttctagt gaaaccccat ttcatttcac tttgcctaaa gaaggtgata tcatcccacc
     3961 attgactggt gcaaccccac ctcttattgg gcacctaaaa ttggagccca agagacacag
     4021 tactcctatt ggtattagca actatccaga aagcaccata gcaaccagtg atgtcatgtc
     4081 tgaaagcatg gtggagaccc atgatcccat acttgggagt ggaaaagggg attctggggc
     4141 tgccccagac gtggatgata aattatgtct aagaatgaaa ctggttagtc ctgagactga
     4201 ggcgagtgaa gagtctttgc agttcaacct ggaaaagcct gcaactggtg aaagaaaaaa
     4261 tggatctact gctgttgctg agtctgttgc cagtccccag aagaccatgt ctgtgttgag
     4321 ctgtatctgt gaagccaggc aagagaatga ggctcgaagt gaggatcccc ccaccacacc
     4381 catcaggggg aacttgctcc actttccaag ttctcaagga gaagaggaga aagaaaaatt
     4441 ggagggtgac catacaatca ggcagagtca acagcctatg aagcccatta gtcctgtcaa
     4501 ggaccctgtt tctcctgctt cccagaagat ggtcatacaa gggccatcca gtcctcaagg
     4561 agaggcaatg gtgacagatg tgctagaaga ccagaaagaa ggacggagta ctaataagga
     4621 aaatcctagt aaggccttga ttgaaaggcc cagccaaaat aacataggaa tccaaaccat
     4681 ggagtgttcc ttgagggtcc cagaaactgt ttcagcagca acccagacta taaagaatgt
     4741 gtgtgagcag gggaccagta cagtggacca gaactttgga aagcaagatg ccacagttca
     4801 gactgagagg gggagtggtg agaaaccagt cagtgctcct ggggatgata cagagtcgct
     4861 ccatagccag ggagaagaag agtttgatat gcctcagcct ccacatggcc atgtcttaca
     4921 tcgtcacatg agaacaatcc gggaagtacg cacacttgtc actcgtgtca ttacagatgt
     4981 gtattatgtg gatggaacag aagtagaaag aaaagtaact gaggagactg aagagccaat
     5041 tgtagagtgt caggagtgtg aaactgaagt ttccccttca cagactgggg gctcctcagg
     5101 tgacctgggg gatatcagct ccttctcctc caaggcatcc agcttacacc gcacatcaag
     5161 tgggacaagt ctctcagcta tgcacagcag tggaagctca gggaaaggag ccggaccact
     5221 cagagggaaa accagcggga cagaacccgc agattttgcc ttacccagct cccgaggagg
     5281 cccaggaaaa ctgagtccta gaaaaggggt cagtcagaca gggacgccag tgtgtgagga
     5341 ggatggtgat gcaggccttg gcatcagaca gggagggaag gctccagtca cgcctcgtgg
     5401 gcgtgggcga aggggccgcc caccttctcg gaccactgga accagagaaa cagctgtgcc
     5461 tggccccttg ggcatagagg acatttcacc taacttgtca ccagatgata aatccttcag
     5521 ccgtgtcgtg ccccgagtgc cagactccac cagacgaaca gatgtgggtg ctggtgcttt
     5581 gcgtcgtagt gactctccag aaattccttt ccaggctgct gctggccctt ctgatggctt
     5641 agatgcctcc tctccaggaa atagctttgt agggctccgt gttgtagcca agtggtcatc
     5701 caatggctac ttttactctg ggaaaatcac acgagatgtc ggagctggga agtataaatt
     5761 gctctttgat gatgggtacg aatgtgatgt gttgggcaaa gacattctgt tatgtgaccc
     5821 catcccgctg gacactgaag tgacggccct ctcggaggat gagtatttca gtgcaggagt
     5881 ggtgaaagga cataggaagg agtctgggga actgtactac agcattgaaa aagaaggcca
     5941 aagaaagtgg tataagcgaa tggctgtcat cctgtccttg gagcaaggaa acagactgag
     6001 agagcagtat gggcttggcc cctatgaagc agtaacacct cttacaaagg cagcagatat
     6061 cagcttagac aatttggtgg aagggaagcg gaaacggcgc agtaacgtca gctccccagc
     6121 cacccctact gcctccagta gcagcagcac aacccctacc cgaaagatca cagaaagtcc
     6181 tcgtgcctcc atgggagttc tctcaggcaa aagaaaactt atcacttctg aagaggaacg
     6241 gtcccctgcc aagcgaggtc gcaagtctgc cacagtaaaa cctggtgcag taggggcagg
     6301 agagtttgtg agcccctgtg agagtggaga caacaccggt gaaccctctg ccctggaaga
     6361 gcagagaggg cctttgcctc tcaacaagac cttgtttctg ggctacgcat ttctccttac
     6421 catggccaca accagtgaca agttggccag ccgctccaaa ctgccagatg gtcctacagg
     6481 aagcagtgaa gaagaggagg aatttttgga aattcctcct ttcaacaagc agtatacaga
     6541 atcccagctt cgagcaggag ctggctatat ccttgaagat ttcaatgaag cccagtgtaa
     6601 cacagcttac cagtgtcttc taattgcgga tcagcattgt cgaacccgga agtacttcct
     6661 gtgccttgcc agtgggattc cttgtgtgtc tcatgtctgg gtccatgata gttgccatgc
     6721 caaccagctc cagaactacc gtaattatct gttgccagct gggtacagcc ttgaggagca
     6781 aagaattctg gactggcaac cccgtgaaaa tcctttccag aatctgaagg tactcttggt
     6841 atcagaccaa cagcagaact tcctggagct ctggtctgag atcctcatga ctggtggtgc
     6901 agcctctgtg aagcagcacc attcaagtgc ccataacaaa gatattgctt taggggtatt
     6961 tgatgtggtg gtgacggacc cctcatgccc agcctcggtg ctgaagtgtg ctgaagcatt
     7021 gcagctgcct gtggtgtcac aagagtgggt gatccagtgc ctcattgttg gggagagaat
     7081 tggattcaag cagcatccaa aatataaaca cgattatgtt tctcactagt aatgaactag
     7141 tccgtaactt gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgaatt
     7201 tgttcgtcta gacgttgata gacgcgttaa gtcgacaatc aacctctgga ttacaaaatt
     7261 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     7321 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     7381 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     7441 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     7501 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     7561 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     7621 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
     7681 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     7741 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     7801 atctcccttt gggccgcctc cccgcgtcga ctttaagacc aatgacttac aaggcagctg
     7861 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     7921 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     7981 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     8041 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     8101 ccttttagtc agtgtggaaa atctctagca gtacgtatag tagttcatgt catcttatta
     8161 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg
     8221 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     8281 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc
     8341 tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     8401 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     8461 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat
     8521 tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac
     8581 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     8641 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     8701 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     8761 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     8821 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     8881 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     8941 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     9001 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     9061 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     9121 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc
     9181 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     9241 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     9301 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     9361 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     9421 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     9481 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     9541 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     9601 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     9661 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     9721 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     9781 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     9841 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     9901 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     9961 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
    10021 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
    10081 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
    10141 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
    10201 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
    10261 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
    10321 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
    10381 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
    10441 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
    10501 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
    10561 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
    10621 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
    10681 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
    10741 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
    10801 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
    10861 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
    10921 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
    10981 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
    11041 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
    11101 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
    11161 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
    11221 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
    11281 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa
    11341 ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt cttatgcaat
    11401 actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac aaggagagaa
    11461 aaagcaccgt gcatgccgat tggtggaagt aaggtggtac gatcgtgcct tattaggaag
    11521 gcaacagacg ggtctgacat ggattggacg aaccactgaa ttgccgcatt gcagagatat
    11581 tgtatttaag tgcctagctc gatacataaa cgggtctctc tggttagacc agatctgagc
    11641 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
    11701 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
    11761 acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg
    11821 aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca
    11881 agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag
    11941 gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa
    12001 aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca
    12061 agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt
    12121 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
    12181 ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc
    12241 aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa
    12301 gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt
    12361 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag
    12421 aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt
    12481 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca
    12541 attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca
    12601 gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt
    12661 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat
    12721 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg
    12781 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
    12841 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
    12901 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
    12961 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
    13021 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
    13081 cccgagggga cccatgcatt gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
    13141 ggctccccag caggcagaag tatgcaaagc atgcgtctca attagtcagc aaccatagtc
    13201 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
    13261 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
    13321 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctttctaga
    13381 ggtaccaccg gagcttacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga
    13441 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
    13501 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
    13561 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgccgtggc
    13621 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
    13681 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
    13741 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
    13801 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
    13861 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
    13921 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
    13981 ccgcaagccc ggtgccagtt aacgaattct agatcttgag acaaatggca gtattcatcc
    14041 acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
    14101 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt
    14161 ttcgggttta ttacagggac agcagagatc cactttggcg ccggctcgag gcctgcaggt
    14221 gcaaagatgg ataaagtttt aaacagagag gaatctttgc agctaatgga ccttctaggt
    14281 cttgaaagga gtgggaatt
//
