LOCUS       sequence-353189-       11638 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11638)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 11638)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2041
FEATURES             Location/Qualifiers
     source          1..11638
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    4576..5164
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4629..4649)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5047..5058)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5236..5469
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5547..5668
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5584..5603)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5638..5657
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5708..5843
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5863..5882)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5864..5882)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5892..5909)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5892..5908)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5901..5923)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6050..6505
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6137..6156)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6347..6368
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6531..6635
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6636..6704,6705..7496)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6854..6873)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7667..8255
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8156..8175
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8409..8426
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8543..8564
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8579..8584,8585..8602,8603..8609)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8617..8633
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8622..8644
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8641..8657
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8641..8657
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8676..8696
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8678..8696
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8724..8950
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8951..9131
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9178..9303
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9796..10029
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10214..10258
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        10489..10699
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        10511..10707
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      10558..10693
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             10739..11341
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(10739..10758)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     11235..11255
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    11384..11501
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgaa
     1201 catggagggt ctggtgatgt tccaagattt aagcatcgac tttagccaag aagagtggga
     1261 gtgtttagat gccgcacaaa aagatttata tcgcgatgtt atgatggaga actatagctc
     1321 tttagtgtct ttaggtttaa gcatcccgaa gccggatgtt atttctttat tagagcaagg
     1381 taaggagccg tggatggtta gtcgcgacgt tttaggtggc tggtgccgcg atagcgaatt
     1441 ccgctgcaaa accaaggata gttgtttacc gaaagagatc tacgaagtga ccagcagtca
     1501 gtgggtgcgc atggaaaaat gccacagtct ggtgggcagc agcgttcgcg atgattggga
     1561 gtgcaaaggc cagttccagc accaagacat taatcaagaa cgttatttag agaaagccat
     1621 tatgacatat gagaccaccc cgaccttctg tttacagacc tctttaacct tgcatcatcg
     1681 catccatccg ggtgagaaac tgtataaaag tacagagtgt atggcattta aatatggtag
     1741 cgagttaacc cagcagcaag aaacccatac tggtgaaaag ctgtataagt gcaaggaatg
     1801 cggcaaagcc tttcaccatt tcagctattt agtgaagcac caacgcattc acaccggtga
     1861 gaagccgtgc gcttgtaagg aatacggcaa agcttttatc agcggcagtc atttaattca
     1921 gcaccagaaa atgtacaccg acgaacgccc tcatgaatgc caagaaagcg tgaaagcatt
     1981 tcgtccgagc gcccatttaa tccaacactg gcgcatccat actggcgata agccgtacga
     2041 atgtaaggaa tgcggcaagt ctttcactag cggcagcacc ttaaaccagc atcaacaaat
     2101 tcacaccggt gagaaaccct accattgtaa gcagtgtggc aaatctttca cagttggtag
     2161 cacattaatt cgtcaccaac agatccacac cggcgaaaag ccttatgact gcaaagaatg
     2221 cggcaagagt tttgccagcg gttctgcttt aatccgccac cagcgcattc atactggtga
     2281 gaagccttat gactgtaaag aatgtggcaa gtccttcacc tttcacagcg ctttaatccg
     2341 ccatcaacgc atccataccg gcgaaaagcc ctatgactgc aaggagtgtg gcaaatcttt
     2401 tacctttcgt agcggtttaa ttggccacca agctatccat actggcgaaa agccttacga
     2461 ctgtaaagag tgtggtaaaa gctttaccgc cggctctacc ttgatccagc accagcgtat
     2521 ccacaccggt gagaagcctt acgattgtaa agagtgtggt aagtccttcg cctctggtag
     2581 cgcattactg caacaccaac gcattcacac tggtgagaaa ccgtattgct gtaaggaatg
     2641 tggcaagtct tttaccttcc gtagcacccg taaccgtcat cagcgcattc acaccggcga
     2701 aaaaccctac aattgtaagg agtgtggcaa aagctttgca agcggcagcg cactgttgca
     2761 gcatcagcgt attcacaccg gcgagaaacc gtatcactgc aaagaatgtg gcaagtcttt
     2821 tacttttcgc agtggtttaa tcggccatca agctgttcat accggcgaga agccttatga
     2881 ttgcaaggaa tgcggtaagt cttttactag tcgcagtgct ttaattcaac accagcgtat
     2941 tcacaccggc gagaagcctt accactgtaa agagtgtggc aagagcttca cagtgggcag
     3001 tactttactg cagcaccaac aaatccatac cggcgaaaaa ccctatgact gcaaagaatg
     3061 tggcaaagct tttcgcttac gcttacgttt aactcagcat cagcaaattc ataccggcga
     3121 aaaaccttac caatgccaag aatgcggcaa ggccttcgtt agcgttagtg gtttaacaca
     3181 acatcaccgt atccataccg gcgagaagcc gtacgagtgt ccggattgtg gcaaagcttt
     3241 ccgccagcgt acatatctga atcaacaccg tcgtattcac actggtgaga aaccctatga
     3301 gtgcaaagaa tgtggtaaaa gttttacatt ctgtagtggc ttaattcaac atcaacagaa
     3361 tcataccgac gaaaagccct acgacggcaa ggaatgcggc aagtctttta ccagccatag
     3421 cactttgatc caacatcaac agatccatac tggtgaaaag ccctacgatt gtaaagagtg
     3481 cggcaaatct tttacaagtc acagtacact gattcaacat caacaaatcc acaccggtga
     3541 aaaactgtat gattgcaagg aatgcggcaa atcttttacc agccacagca ccttaattca
     3601 gcatcagcct ctgcatactg gcgagaagcc ctaccattgt aaggagtgtg gtaagtcctt
     3661 cactttacgc agtgctttaa ttcaacaccg tccggttcac actggtgaga aacgctacag
     3721 ctgtaaagaa tgtggcaaat cctttaccag ccgcagcaca ctgattgaac atcaacgtat
     3781 tcatactggt gagaaacctt atcattgtaa agagtgcggt aaatctttcg ccttccgcag
     3841 cgccatcatt cagcaccgcc gcatccatac cggcgagaag ccttacgact gcaaggaatg
     3901 cggtaaggct ttccgccgtc gtagcaagct gacacagcat caacgtatcc acactggcga
     3961 aaagccctat cgttgtcacg aatgcggtaa agcattcgtg cgctttagcg gcttaaccaa
     4021 gcatcatagc attcacactg gtgagaagcc ctacgagtgc aaaacttgtg gtaaaagttt
     4081 ccgtcagcgc acccatctga cactgcacca acgcatccac accggtgatc gcccgtatga
     4141 gtgtaaggag tgcggcaaat ctttcacttg tggcagcgaa ctgattcgtc accagcgtac
     4201 acacactggt gaaaaaccct acgactgcaa ggaatgcggt aaggcatttc gttgcccgag
     4261 tcagctgagt cagcataaac gtattcatac tggtgaaaag acctatcaat gtccggagtg
     4321 tggtaaagcc ttcttttatg caagcggtct gagccgccac cagagtgttc acaccggcga
     4381 aaagccttac gagtgtaaaa cttgtggtaa ggccttcaag cagctgaccc agctgacacg
     4441 ccaccaacgc attcatgact taacctaata atgaactagt ccgtaacttg aaagtatttc
     4501 gatttcttgg ctttatatat cttgtggaaa ggacgaggtt ggctatatgt ttgtgctttg
     4561 acgcgttaag tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     4621 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     4681 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     4741 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     4801 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     4861 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     4921 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     4981 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     5041 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     5101 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     5161 ccgcgtcgac tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     5221 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg
     5281 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     5341 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     5401 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     5461 tctctagcag tacgtatagt agttcatgtc atcttattat tcagtattta taacttgcaa
     5521 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat
     5581 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     5641 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac
     5701 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     5761 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     5821 gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat
     5881 tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     5941 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     6001 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt
     6061 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     6121 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     6181 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     6241 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     6301 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     6361 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     6421 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     6481 aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc
     6541 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     6601 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     6661 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     6721 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     6781 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     6841 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     6901 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     6961 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     7021 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     7081 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     7141 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     7201 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     7261 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     7321 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     7381 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     7441 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     7501 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     7561 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     7621 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     7681 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     7741 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     7801 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     7861 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     7921 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     7981 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     8041 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     8101 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     8161 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     8221 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     8281 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     8341 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     8401 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     8461 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     8521 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     8581 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     8641 caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac
     8701 aaaagctgga gctgcaagct taatgtagtc ttatgcaata ctcttgtagt cttgcaacat
     8761 ggtaacgatg agttagcaac atgccttaca aggagagaaa aagcaccgtg catgccgatt
     8821 ggtggaagta aggtggtacg atcgtgcctt attaggaagg caacagacgg gtctgacatg
     8881 gattggacga accactgaat tgccgcattg cagagatatt gtatttaagt gcctagctcg
     8941 atacataaac gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     9001 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     9061 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     9121 aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc
     9181 tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg
     9241 gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc
     9301 gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg
     9361 ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc
     9421 gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta
     9481 caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc
     9541 ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata
     9601 gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc
     9661 tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa
     9721 aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa
     9781 aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat
     9841 gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca
     9901 gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt
     9961 ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca
    10021 acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg
    10081 gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga
    10141 gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca
    10201 aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg
    10261 gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg
    10321 aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca
    10381 gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccatgcattg
    10441 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
    10501 atgcaaagca tgcgtctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
    10561 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
    10621 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
    10681 ttttttggag gcctaggctt ttgcaaaaag ctttctagag gtaccaccgg agcttaccat
    10741 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg
    10801 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg
    10861 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
    10921 cggcaaggtg tgggtcgcgg acgacggcgc cgccgtggcg gtctggacca cgccggagag
    10981 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
    11041 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
    11101 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
    11161 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
    11221 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
    11281 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgccagtta
    11341 acgaattcta gatcttgaga caaatggcag tattcatcca caattttaaa agaaaagggg
    11401 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
    11461 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
    11521 gcagagatcc actttggcgc cggctcgagg cctgcaggtg caaagatgga taaagtttta
    11581 aacagagagg aatctttgca gctaatggac cttctaggtc ttgaaaggag tgggaatt
//
