LOCUS       sequence-35319-e         904 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 904)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 904)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: HL 2818 (=HL 865 + pHL 489)
FEATURES             Location/Qualifiers
     source          1..904
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    205..223
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    230..248
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     complement(269..287)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        355..459
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(678..697)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 ttcttctcct ttactatcat gaactttcag cgtattctga ctcataaggt ggctcctacc
       61 cgtgatccct tgacggaaca ttcaagcaaa agcctggttc cgccgattta tcgctgcggc
      121 aaataacgca gcgggtttac ggatttcccc ttgtaacgaa tttcaaaatg caagcgtgtt
      181 gaactggttc cggtgtcgac agtatctcta tcactgatag ggatgtcaat ctctatcact
      241 gatagggact cgaggtgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat
      301 gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
      361 acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
      421 ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
      481 gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
      541 ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
      601 gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
      661 agcactttta aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag
      721 caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
      781 gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
      841 agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
      901 gctt
//
