LOCUS       sequence-353191-       11542 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11542)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 11542)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2042
FEATURES             Location/Qualifiers
     source          1..11542
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    4480..5068
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4533..4553)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4951..4962)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5140..5373
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5451..5572
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5488..5507)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5542..5561
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5612..5747
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5767..5786)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5768..5786)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5796..5813)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5796..5812)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5805..5827)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5954..6409
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6041..6060)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6251..6272
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6435..6539
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6540..6608,6609..7400)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6758..6777)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7571..8159
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8060..8079
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8313..8330
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8447..8468
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8483..8488,8489..8506,8507..8513)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8521..8537
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8526..8548
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8545..8561
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8545..8561
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8580..8600
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8582..8600
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8628..8854
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8855..9035
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9082..9207
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9700..9933
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10118..10162
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        10393..10603
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        10415..10611
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      10462..10597
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             10643..11245
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(10643..10662)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     11139..11159
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    11288..11405
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgaa
     1201 catggagggt ttagttatgt ttcaagattt aagcatcgat ttcagccaag aagaatggga
     1261 gtgtttagat gccgcccaga aggatctgta tcgcgacgtg atgatggaga attacagctc
     1321 tttagtttct ttagacagtg agttccgctg taaaaccaaa gacagttgtt taccgaaaga
     1381 aatctatgag gttaccagca gccaatgggt gcgtatggag aaatgccata gtctggtggg
     1441 cagcagtgtt cgtgatgatt gggagtgcaa aggccagttc caacatcaag atattaatca
     1501 agagcgctac ttagagaagg ccatcatgac ctacgagacc accccgacat tttgcttaca
     1561 gacaagttta accttacatc atcgcatcca tccgggtgag aagctgtata aaagtaccga
     1621 gtgtatggcc ttcaagtacg gtagcgagct gacccagcag caagaaaccc atactggtga
     1681 aaagctgtac aagtgcaagg agtgcggcaa agcctttcat catttcagct atttagttaa
     1741 acatcagcgt atccacaccg gcgagaaacc gtgcgcttgt aaagagtatg gcaaggcctt
     1801 tatcagcggt agccatttaa tccagcacca gaagatgtac accgatgaac gtccgcatga
     1861 atgccaagaa agtgtcaagg ccttccgccc gagcgcccac ttaattcaac actggcgtat
     1921 tcataccggt gataaaccct atgagtgtaa agagtgcggc aagagtttca ccagcggcag
     1981 caccttaaat cagcaccagc aaatccacac tggcgaaaag ccgtaccatt gcaagcaatg
     2041 tggtaagtct ttcaccgtgg gttctacttt aattcgtcat caacagatcc acaccggcga
     2101 gaaaccttac gattgtaaag aatgtggtaa gagcttcgca agtggtagcg ccttaatccg
     2161 tcatcaacgt atccataccg gcgaaaaacc ttatgactgt aaggaatgtg gcaagagctt
     2221 tacctttcat agcgcactga ttcgtcacca acgcatccac accggtgaga agccttatga
     2281 ctgtaaggag tgtggtaaga gcttcacctt tcgcagcggc ttaatcggtc atcaagctat
     2341 tcacaccggt gaaaagccct acgattgcaa ggagtgtggt aagagtttta cagctggtag
     2401 cactttaatt caacaccaac gcatccatac tggcgaaaag ccttatgact gcaaagaatg
     2461 cggcaagagc tttgccagcg gtagcgcttt attacaacac caacgcattc acactggcga
     2521 aaagccttat tgttgcaagg aatgtggtaa atcttttaca ttccgtagca cccgcaatcg
     2581 ccaccaacgt atccacactg gtgagaagcc gtacaattgt aaggagtgcg gcaagagctt
     2641 cgccagtggt agcgcattac tgcaacacca gcgcattcat accggcgaaa aaccttatca
     2701 ctgcaaggag tgtggtaaat cctttacctt tcgtagcggt ttaatcggcc accaagctgt
     2761 tcatactggt gaaaagcctt acgactgcaa agaatgtggt aagtctttta ccagccgctc
     2821 tgctttaatc cagcaccaac gcattcatac cggcgaaaaa ccctaccatt gcaaagaatg
     2881 tggcaaatcc ttcaccgttg gtagtacttt actgcagcat cagcagattc ataccggtga
     2941 gaaaccctat gattgcaaag agtgcggcaa ggcttttcgc ttacgtttac gtttaaccca
     3001 acatcagcaa attcacactg gtgagaaacc gtaccaatgt caagaatgtg gtaaagcatt
     3061 tgtgagcgtt agtggtttaa cacagcacca tcgtatccac actggtgaga agccctacga
     3121 gtgccccgat tgcggtaagg ctttccgcca gcgcacctat ttaaatcaac atcgtcgtat
     3181 ccacactggt gagaagcctt acgagtgcaa agaatgtggc aagtctttta ccttctgcag
     3241 tggcttaatc caacaccaac agaaccacac cgacgagaag ccctatgatg gtaaagaatg
     3301 tggcaagagc ttcacaagcc acagcacttt aattcaacat caacaaatcc acactggtga
     3361 gaagccctac gactgcaagg agtgcggcaa atccttcacc agccatagta cactgattca
     3421 acaccaacaa atccatactg gcgagaaact gtatgattgc aaagaatgtg gtaagtcttt
     3481 caccagtcat tctactttga ttcagcacca gcctttacat actggtgaga aaccttacca
     3541 ttgcaaagag tgcggcaagt cctttacctt acgcagcgct ctgatccaac accgcccggt
     3601 gcacactggt gaaaaacgct attcttgtaa agaatgtggc aaatctttta cttctcgcag
     3661 tactctgatt gagcaccagc gcatccacac tggtgagaaa ccgtatcatt gtaaggagtg
     3721 tggtaagagt ttcgccttcc gcagtgcaat tatccagcac cgccgtatcc acactggcga
     3781 gaaaccctac gattgtaaag aatgcggtaa agcattccgt cgccgtagca aactgactca
     3841 gcatcaacgc atccacactg gtgagaaacc ttaccgttgc catgagtgcg gcaaagcttt
     3901 tgtgcgcttc agcggtctga caaaacacca tagcatccat accggcgaga aaccctatga
     3961 atgcaagact tgtggtaaaa gttttcgcca gcgtacacac ttaactttac atcaacgtat
     4021 tcacactggc gatcgtcctt acgagtgtaa ggaatgcggc aagtccttca cttgcggtag
     4081 tgagctgatc cgccatcagc gcacacacac cggcgaaaag ccttacgatt gcaaagagtg
     4141 tggtaaagct tttcgttgcc cgagccagct gagtcaacac aagcgcatcc ataccggtga
     4201 aaaaacatac cagtgtccgg aatgcggcaa ggccttcttc tacgccagtg gtctgagtcg
     4261 ccaccaaagc gtccacaccg gtgaaaagcc gtatgagtgt aaaacatgtg gcaaagcctt
     4321 caagcaactg acccaactga cacgccacca acgtatccat gatctgacat aataatgaac
     4381 tagtccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga
     4441 tagtcacatt aacatgaacc agcaacgcgt taagtcgaca atcaacctct ggattacaaa
     4501 atttgtgaaa gattgactgg tattcttaac tatgttgctc cttttacgct atgtggatac
     4561 gctgctttaa tgcctttgta tcatgctatt gcttcccgta tggctttcat tttctcctcc
     4621 ttgtataaat cctggttgct gtctctttat gaggagttgt ggcccgttgt caggcaacgt
     4681 ggcgtggtgt gcactgtgtt tgctgacgca acccccactg gttggggcat tgccaccacc
     4741 tgtcagctcc tttccgggac tttcgctttc cccctcccta ttgccacggc ggaactcatc
     4801 gccgcctgcc ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg
     4861 gtgttgtcgg ggaaatcatc gtcctttcct tggctgctcg cctgtgttgc cacctggatt
     4921 ctgcgcggga cgtccttctg ctacgtccct tcggccctca atccagcgga ccttccttcc
     4981 cgcggcctgc tgccggctct gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt
     5041 cggatctccc tttgggccgc ctccccgcgt cgactttaag accaatgact tacaaggcag
     5101 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
     5161 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
     5221 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
     5281 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
     5341 gaccctttta gtcagtgtgg aaaatctcta gcagtacgta tagtagttca tgtcatctta
     5401 ttattcagta tttataactt gcaaagaaat gaatatcaga gagtgagagg aacttgttta
     5461 ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     5521 ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct
     5581 ggctctagct atcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     5641 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     5701 ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag ggacgtaccc
     5761 aattcgccct atagtgagtc gtattacgcg cgctcactgg ccgtcgtttt acaacgtcgt
     5821 gactgggaaa accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc
     5881 agctggcgta atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg
     5941 aatggcgaat gggacgcgcc ctgtagcggc gcattaagcg cggcgggtgt ggtggttacg
     6001 cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc tttcttccct
     6061 tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg gctcccttta
     6121 gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta gggtgatggt
     6181 tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt ggagtccacg
     6241 ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat ctcggtctat
     6301 tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa tgagctgatt
     6361 taacaaaaat ttaacgcgaa ttttaacaaa atattaacgc ttacaattta ggtggcactt
     6421 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     6481 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     6541 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     6601 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     6661 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     6721 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     6781 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     6841 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     6901 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     6961 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     7021 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     7081 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     7141 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     7201 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     7261 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     7321 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     7381 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     7441 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     7501 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     7561 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     7621 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     7681 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
     7741 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     7801 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     7861 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     7921 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     7981 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     8041 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     8101 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     8161 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     8221 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     8281 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     8341 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     8401 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     8461 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     8521 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcgcg
     8581 caattaaccc tcactaaagg gaacaaaagc tggagctgca agcttaatgt agtcttatgc
     8641 aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct tacaaggaga
     8701 gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg ccttattagg
     8761 aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc attgcagaga
     8821 tattgtattt aagtgcctag ctcgatacat aaacgggtct ctctggttag accagatctg
     8881 agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc
     8941 ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct
     9001 cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag ggacttgaaa
     9061 gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga agcgcgcacg
     9121 gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag cggaggctag
     9181 aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag atcgcgatgg
     9241 gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca tatagtatgg
     9301 gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac atcagaaggc
     9361 tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga agaacttaga
     9421 tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga gataaaagac
     9481 accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac caccgcacag
     9541 caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt ggagaagtga
     9601 attatataaa tataaagtag taaaaattga accattagga gtagcaccca ccaaggcaaa
     9661 gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt tccttgggtt
     9721 cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg tacaggccag
     9781 acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta ttgaggcgca
     9841 acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa gaatcctggc
     9901 tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct ctggaaaact
     9961 catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc tggaacagat
    10021 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
    10081 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
    10141 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
    10201 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
    10261 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
    10321 aaccccgagg ggacccatgc attgcatctc aattagtcag caaccaggtg tggaaagtcc
    10381 ccaggctccc cagcaggcag aagtatgcaa agcatgcgtc tcaattagtc agcaaccata
    10441 gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg
    10501 ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag
    10561 ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctttct
    10621 agaggtacca ccggagctta ccatgaccga gtacaagccc acggtgcgcc tcgccacccg
    10681 cgacgacgtc cccagggccg tacgcaccct cgccgccgcg ttcgccgact accccgccac
    10741 gcgccacacc gtcgatccgg accgccacat cgagcgggtc accgagctgc aagaactctt
    10801 cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg gcgccgccgt
    10861 ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg agatcggccc
    10921 gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg aaggcctcct
    10981 ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg tctcgcccga
    11041 ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg cggccgagcg
    11101 cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct tctacgagcg
    11161 gctcggcttc accgtcaccg ccgacgtcga ggtgcccgaa ggaccgcgca cctggtgcat
    11221 gacccgcaag cccggtgcca gttaacgaat tctagatctt gagacaaatg gcagtattca
    11281 tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa agaatagtag
    11341 acataatagc aacagacata caaactaaag aattacaaaa acaaattaca aaaattcaaa
    11401 attttcgggt ttattacagg gacagcagag atccactttg gcgccggctc gaggcctgca
    11461 ggtgcaaaga tggataaagt tttaaacaga gaggaatctt tgcagctaat ggaccttcta
    11521 ggtcttgaaa ggagtgggaa tt
//
