LOCUS       sequence-353195-       11578 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11578)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 11578)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2115
FEATURES             Location/Qualifiers
     source          1..11578
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1190..1199
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    4516..5104
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4569..4589)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4987..4998)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5176..5409
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5487..5608
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5524..5543)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5578..5597
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5648..5783
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5803..5822)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5804..5822)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5832..5849)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5832..5848)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5841..5863)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5990..6445
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6077..6096)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6287..6308
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6471..6575
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6576..6644,6645..7436)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6794..6813)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7607..8195
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8096..8115
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8349..8366
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8483..8504
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8519..8524,8525..8542,8543..8549)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8557..8573
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8562..8584
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8581..8597
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8581..8597
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8616..8636
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8618..8636
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8664..8890
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8891..9071
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9118..9243
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9736..9969
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10154..10198
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        10429..10639
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        10451..10647
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      10498..10633
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             10679..11281
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(10679..10698)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     11175..11195
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    11324..11441
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatggt
     1201 tttggatgac cttccaaact tagaagacat ctatacttcc ttgtgttcat caacaatgga
     1261 agactcagag atggattttg actctggact agaagatgat gacacaaaaa gtgatagtat
     1321 tttggaggat tccacaattt ttgtggcctt caaaggaaat atagatgata aagacttcaa
     1381 atggaaatta gatgcaatat tgaaaaacgt gcccaatttg ttacacatgg agtccagcaa
     1441 gctaaaagta cagaaggtgg agccctggaa cagcgtgcgt gtgacattca acatcccccg
     1501 ggaagcagcg gagcggctac ggatccttgc tcagagcaac aaccagcagc ttcgggattt
     1561 agggattctc tccgttcaga ttgaagggga aggtgctatt aacctggctt tggctcagaa
     1621 ccgaagccaa gatgtgagaa tgaatggacc catgggagct ggaaattcag ttaggatgga
     1681 ggcgggattt cctatggcaa gtggtccagg aataataagg atgaacaacc ctgccactgt
     1741 tatgataccc ccgggtggaa atgtgtcatc ttccatgatg gcaccaggcc ccaatccaga
     1801 gctgcagccc aggactcctc gccctgcttc tcagtcagat gcaatggatc cactcctctc
     1861 tgggctccat atacagcagc aaagtcatcc ctcaggatct ttagctcccc cacatcaccc
     1921 aatgcagcct gtctctgtga acagacaaat gaacccagct aattttcccc agctgcagca
     1981 gcagcagcaa caacaacaac agcagcagca gcagcagcag cagcaacaac agcaacagca
     2041 gcaacaacag ttgcaggcaa gacccccaca gcaacatcag cagcaacagc cacagggaat
     2101 tcgaccccag tttactgccc caactcaggt gcctgttcct ccaggctgga accagctgcc
     2161 ttctggagcc cttcaacctc ctccagccca gggttctctg ggcacaatga ctgcaaacca
     2221 agggtggaag aaggctccct tgcccggccc aatgcaacag caactccagg caagaccatc
     2281 cttagccacg gtacagacgc cttcccaccc tccccctcca tatccctttg gcagccagca
     2341 agcctcacaa gcccacacaa actttcctca gatgagcaac ccaggccagt tcacagctcc
     2401 tcagatgaag agtttgcagg gagggccctc tagggtccca actcccttgc agcagcccca
     2461 cctcaccaac aagtctcctg cctcctcacc ctcctccttc cagcagggat cccctgcatc
     2521 ctccccaacg gttaaccaaa ctcagcagca gatgggacca aggccacctc aaaataaccc
     2581 acttccccag ggatttcagc agcctgtcag ctctccgggt cggaatccta tggttcaaca
     2641 gggaaatgtg ccacctaact tcatggtgat gcagcagcaa ccaccaaacc aggggccaca
     2701 gagtttacat ccaggcctag gaggaatgcc taaacgcctc ccacctggct tctcagcagg
     2761 acaggccaat ccgaacttta tgcaaggtca ggtgccttcg accacagcaa ccacccctgg
     2821 gaattcagga gcccctcagc tgcaagcaaa tcaaaatgtc cagcatgcag gtggtcaagg
     2881 agctggtcct cctcaaaacc agatgcaggt gtcccacggg ccgccaaata tgatgcagcc
     2941 cagcctcatg ggaattcatg gcaacatgaa caatcagcag gctggtactt ctggggttcc
     3001 tcaagtgaac ctcagcaaca tgcaaggcca gccccagcag ggcccaccat ctcagctgat
     3061 gggcatgcac cagcaaatcg tgccctccca gggccagatg gtccagcaac aaggaacctt
     3121 gaaccctcag aaccctatga tcctttcaag ggcccagctt atgccacagg gccagatgat
     3181 ggtgaacccc ccgagccaaa atcttgggcc ctcgccccaa aggatgaccc cacccaagca
     3241 gatgctttcc cagcagggcc cacaaatgat ggcgccacat aaccagatga tggggcctca
     3301 ggggcaggtt ttgctccaac agaacccaat gatagagcag attatgacca atcaaatgca
     3361 ggggaataag cagcagttta acactcagaa ccagtccaat gtcatgccgg gaccagccca
     3421 gataatgagg ggaccaactc caaacatgca aggaaatatg gtgcagttta cgggacagat
     3481 gtcaggacag atgctgcccc agcaagggcc tgtgaacaac agtccatctc aggttatggg
     3541 cattcaggga caggtcctgc ggccaccagg gcccagccca cacatggccc agcagcatgg
     3601 tgatcctgct actacagcaa ataacgatgt cagtttatct cagatgatgc ctgatgttag
     3661 cattcaacaa accaacatgg tcccccctca tgtgcaggcc atgcagggaa acagtgcctc
     3721 gggaaaccac ttctcaggcc atgggatgtc tttcaatgca cctttcagtg gagctcccaa
     3781 tggaaatcag atgtcctgtg gtcaaaatcc aggcttccca gtcaataagg atgtcacgct
     3841 aacgagccca ttgttggtca acttattgca gagtgacata tctgcaggcc attttggggt
     3901 aaacaataag caaaataata ccaacgcaaa taaaccgaag aagaagaaac cccctcggaa
     3961 gaagaaaaat agtcagcaag atctaaacac cccagatact cgcccagctg gtctggaaga
     4021 ggctgatcag ccaccgttgc ctggagaaca aggaattaac ttggataact caggccctaa
     4081 actgccagaa ttttcaaacc ggccaccagc cccgtcgcag aatttagtct caaaggaaac
     4141 ttcaaccaca gcactgcagg cctctgttgc cagaccagag ctggaggtaa atgctgccat
     4201 agtctctgga caaagcagtg agcccaaaga gatagttgaa aagtccaaaa tcccaggccg
     4261 aagaaactcc cgaactgaag agccaactgt ggcctctgaa agtgtggaaa atggacatcg
     4321 taaacgatct tctcgacctg cttcagcctc cagctctact aaagacataa ccagtgcggt
     4381 gcaatccaag cgaagaaaat ccaagtagta atgaactagt ccgtaacttg aaagtatttc
     4441 gatttcttgg ctttatatat cttgtggaaa ggacgattgc acagtaacgc gtactggtcc
     4501 acgcgttaag tcgacaatca acctctggat tacaaaattt gtgaaagatt gactggtatt
     4561 cttaactatg ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat
     4621 gctattgctt cccgtatggc tttcattttc tcctccttgt ataaatcctg gttgctgtct
     4681 ctttatgagg agttgtggcc cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct
     4741 gacgcaaccc ccactggttg gggcattgcc accacctgtc agctcctttc cgggactttc
     4801 gctttccccc tccctattgc cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg
     4861 acaggggctc ggctgttggg cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc
     4921 tttccttggc tgctcgcctg tgttgccacc tggattctgc gcgggacgtc cttctgctac
     4981 gtcccttcgg ccctcaatcc agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg
     5041 cctcttccgc gtcttcgcct tcgccctcag acgagtcgga tctccctttg ggccgcctcc
     5101 ccgcgtcgac tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     5161 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg
     5221 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     5281 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     5341 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     5401 tctctagcag tacgtatagt agttcatgtc atcttattat tcagtattta taacttgcaa
     5461 agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat
     5521 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
     5581 gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac
     5641 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     5701 aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta
     5761 gtgaggaggc ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat
     5821 tacgcgcgct cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc
     5881 caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc
     5941 cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt
     6001 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
     6061 agcgccctag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
     6121 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
     6181 cacctcgacc ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga
     6241 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
     6301 caaactggaa caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg
     6361 ccgatttcgg cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
     6421 aacaaaatat taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc
     6481 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     6541 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     6601 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     6661 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     6721 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     6781 cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac
     6841 tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     6901 agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     6961 ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     7021 ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     7081 aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc
     7141 gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     7201 tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     7261 ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     7321 cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     7381 atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     7441 cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     7501 ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     7561 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     7621 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     7681 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     7741 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     7801 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     7861 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     7921 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     7981 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     8041 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     8101 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     8161 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     8221 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     8281 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     8341 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     8401 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     8461 cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt
     8521 tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca
     8581 caggaaacag ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac
     8641 aaaagctgga gctgcaagct taatgtagtc ttatgcaata ctcttgtagt cttgcaacat
     8701 ggtaacgatg agttagcaac atgccttaca aggagagaaa aagcaccgtg catgccgatt
     8761 ggtggaagta aggtggtacg atcgtgcctt attaggaagg caacagacgg gtctgacatg
     8821 gattggacga accactgaat tgccgcattg cagagatatt gtatttaagt gcctagctcg
     8881 atacataaac gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact
     8941 agggaaccca ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc
     9001 ccgtctgttg tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa
     9061 aatctctagc agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc
     9121 tctcgacgca ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg
     9181 gtgagtacgc caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc
     9241 gtcagtatta agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg
     9301 ggaaagaaaa aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc
     9361 gcagttaatc ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta
     9421 caaccatccc ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc
     9481 ctctattgtg tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata
     9541 gaggaagagc aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc
     9601 tggaggagga gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa
     9661 aattgaacca ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa
     9721 aagagcagtg ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat
     9781 gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca
     9841 gcagcagaac aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt
     9901 ctggggcatc aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca
     9961 acagctcctg gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg
    10021 gaatgctagt tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga
    10081 gtgggacaga gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca
    10141 aaaccagcaa gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg
    10201 gaattggttt aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg
    10261 aggcttggta ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca
    10321 gggatattca ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccatgcattg
    10381 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
    10441 atgcaaagca tgcgtctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
    10501 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
    10561 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
    10621 ttttttggag gcctaggctt ttgcaaaaag ctttctagag gtaccaccgg agcttaccat
    10681 gaccgagtac aagcccacgg tgcgcctcgc cacccgcgac gacgtcccca gggccgtacg
    10741 caccctcgcc gccgcgttcg ccgactaccc cgccacgcgc cacaccgtcg atccggaccg
    10801 ccacatcgag cgggtcaccg agctgcaaga actcttcctc acgcgcgtcg ggctcgacat
    10861 cggcaaggtg tgggtcgcgg acgacggcgc cgccgtggcg gtctggacca cgccggagag
    10921 cgtcgaagcg ggggcggtgt tcgccgagat cggcccgcgc atggccgagt tgagcggttc
    10981 ccggctggcc gcgcagcaac agatggaagg cctcctggcg ccgcaccggc ccaaggagcc
    11041 cgcgtggttc ctggccaccg tcggcgtctc gcccgaccac cagggcaagg gtctgggcag
    11101 cgccgtcgtg ctccccggag tggaggcggc cgagcgcgcc ggggtgcccg ccttcctgga
    11161 gacctccgcg ccccgcaacc tccccttcta cgagcggctc ggcttcaccg tcaccgccga
    11221 cgtcgaggtg cccgaaggac cgcgcacctg gtgcatgacc cgcaagcccg gtgccagtta
    11281 acgaattcta gatcttgaga caaatggcag tattcatcca caattttaaa agaaaagggg
    11341 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
    11401 ctaaagaatt acaaaaacaa attacaaaaa ttcaaaattt tcgggtttat tacagggaca
    11461 gcagagatcc actttggcgc cggctcgagg cctgcaggtg caaagatgga taaagtttta
    11521 aacagagagg aatctttgca gctaatggac cttctaggtc ttgaaaggag tgggaatt
//
