LOCUS       sequence-35320-e         826 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: HL 2817 (=HL 716 + pHL 720)
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    140..158
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    165..183
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     terminator      complement(250..277)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(369..455)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     608..625
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     primer_bind     complement(711..731)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 tcgcctcgcc tcgatctcga actcgtggcc gttcacggag ccctccatgt gcaccttgaa
       61 gcgcatgaac tccttgatga tggccatgtt atcctcctcg cccttgctca ccattttttt
      121 tcctccttac ccgggagtat ctctatcact gatagggatg tcaatctcta tcactgatag
      181 ggaggatcct gagcggatac atatttgaat gtatttagaa aaataaacaa aagagtttgt
      241 agaaacgcaa aaaggccatc cgtcaggatg gccttctgct taatttgatg cctggcagtt
      301 tatggcgggc gtcctgcccg ccaccctccg ggccgttgct tcgcaacgtt caaatccgct
      361 cccggcggat ttgtcctact caggagagcg ttcaccgaca aacaacagat aaaacgaaag
      421 gcccagtctt tcgactgagc ctttcgtttt atttgatgcc tggcagttcc ctactctcgc
      481 atggggagac cccacactac catcggcgct acggcgtttc acttctgagt tcggcatggg
      541 gtcaggtggg accaccgcgc tactgccgcc aggcaaattc tgttttatca gaccgcttct
      601 gcgttctgat ttaatctgta tcaggctgaa aatcttctct catccgccaa aacagccaag
      661 ctagctttta tttgtagagc tcatccatgc catgtgtaat cccagcagct gttacaaact
      721 caagaaggac catgtggtct ctcttttcgt tgggatcttt cgaaagggca gattgtgtgg
      781 acaggtaatg gttgtctggt aaaaggacag ggccatcgcc aattgg
//
