LOCUS       sequence-353206-        9139 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9139)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 9139)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2559
FEATURES             Location/Qualifiers
     source          1..9139
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     regulatory      1190..1199
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    2077..2665
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2130..2150)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2548..2559)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             2737..2970
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3048..3169
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3085..3104)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3139..3158
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      3209..3344
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3364..3383)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3365..3383)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3393..3410)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(3393..3409)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3402..3424)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3551..4006
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3638..3657)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3848..3869
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4032..4136
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4137..4205,4206..4997)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4355..4374)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      5168..5756
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5657..5676
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5910..5927
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6044..6065
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6080..6085,6086..6103,6104..6110)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6118..6134
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6123..6145
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6142..6158
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6142..6158
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     6177..6197
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6179..6197
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        6225..6451
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             6452..6632
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    6679..6804
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    7297..7530
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             7715..7759
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        7990..8200
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8012..8208
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8059..8194
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8240..8842
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(8240..8259)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8736..8756
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    8885..9002
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgga
     1201 acatctgggc ccgcatcatc tgcatccggg tcacgccgaa ccgatcagct tcggtatcga
     1261 tcagattctg aacagcccag atcaaggcgg ctgcatgggt ccagcgagcc gtctccaaga
     1321 tggcgaatac ggtctgggtt gtctggtggg tggcgcgtac acgtatggtg gtggtggtag
     1381 cgccgcggcc accggcgcgg gcggcgcggg cgcctacggt acgggtggtc cgggcggccc
     1441 gggcggtccg gcgggcggtg gcggtgcgtg cagtatgggt ccgctgaccg gcagctacaa
     1501 tgtgaatatg gcgctggccg gcggcccggg cccgggcggc ggtggtggta gtagcggtgg
     1561 tgccggtgcg ctgagcgccg cgggtgttat tcgcgttcca gcccatcgtc cactggcggg
     1621 tgcggttgcc catccacaac cactggcgac gggtctgccg accgttccga gtgttccggc
     1681 catgccgggc gttaacaatc tgaccggtct gacctttccg tggatggaaa gcaaccgccg
     1741 ctacaccaag gatcgcttca ccggccatcc gtatcagaat cgcaccccgc cgaagaagaa
     1801 aaaaccgcgc accagcttca cccgtctgca gatctgcgaa ctggaaaaac gcttccaccg
     1861 ccagaaatat ctggccagcg cggaacgcgc ggcgctggcg aaagcgctga aaatgacgga
     1921 tgcccaagtt aaaacgtggt tccagaaccg ccgcaccaaa tggcgctaat aatgaactag
     1981 tccgtaactt gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgagcc
     2041 cggcctttga gtccctccgg gacgcgttaa gtcgacaatc aacctctgga ttacaaaatt
     2101 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     2161 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     2221 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     2281 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     2341 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     2401 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     2461 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
     2521 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     2581 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     2641 atctcccttt gggccgcctc cccgcgtcga ctttaagacc aatgacttac aaggcagctg
     2701 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     2761 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     2821 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     2881 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     2941 ccttttagtc agtgtggaaa atctctagca gtacgtatag tagttcatgt catcttatta
     3001 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg
     3061 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     3121 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc
     3181 tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     3241 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     3301 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat
     3361 tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac
     3421 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     3481 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     3541 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     3601 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     3661 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     3721 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     3781 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     3841 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     3901 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     3961 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc
     4021 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     4081 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     4141 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     4201 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     4261 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     4321 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     4381 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     4441 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     4501 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     4561 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     4621 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     4681 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     4741 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     4801 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     4861 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     4921 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     4981 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     5041 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     5101 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     5161 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     5221 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     5281 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     5341 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     5401 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     5461 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     5521 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     5581 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     5641 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     5701 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     5761 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     5821 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     5881 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     5941 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     6001 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     6061 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     6121 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa
     6181 ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt cttatgcaat
     6241 actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac aaggagagaa
     6301 aaagcaccgt gcatgccgat tggtggaagt aaggtggtac gatcgtgcct tattaggaag
     6361 gcaacagacg ggtctgacat ggattggacg aaccactgaa ttgccgcatt gcagagatat
     6421 tgtatttaag tgcctagctc gatacataaa cgggtctctc tggttagacc agatctgagc
     6481 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     6541 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     6601 acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg
     6661 aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca
     6721 agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag
     6781 gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa
     6841 aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca
     6901 agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt
     6961 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
     7021 ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc
     7081 aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa
     7141 gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt
     7201 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag
     7261 aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt
     7321 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca
     7381 attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca
     7441 gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt
     7501 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat
     7561 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg
     7621 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
     7681 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
     7741 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
     7801 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
     7861 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
     7921 cccgagggga cccatgcatt gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     7981 ggctccccag caggcagaag tatgcaaagc atgcgtctca attagtcagc aaccatagtc
     8041 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     8101 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     8161 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctttctaga
     8221 ggtaccaccg gagcttacca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga
     8281 cgacgtcccc agggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
     8341 ccacaccgtc gatccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
     8401 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgccgtggc
     8461 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
     8521 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
     8581 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
     8641 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
     8701 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
     8761 cggcttcacc gtcaccgccg acgtcgaggt gcccgaagga ccgcgcacct ggtgcatgac
     8821 ccgcaagccc ggtgccagtt aacgaattct agatcttgag acaaatggca gtattcatcc
     8881 acaattttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga atagtagaca
     8941 taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa attcaaaatt
     9001 ttcgggttta ttacagggac agcagagatc cactttggcg ccggctcgag gcctgcaggt
     9061 gcaaagatgg ataaagtttt aaacagagag gaatctttgc agctaatgga ccttctaggt
     9121 cttgaaagga gtgggaatt
//
