LOCUS       sequence-35321-e         864 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 864)
  AUTHORS   Hussein R, Lim HN
  TITLE     Disruption of small RNA signaling caused by competition for Hfq.
  JOURNAL   Proc Natl Acad Sci U S A. 2011 Jan 18;108(3):1110-5. doi: 
            10.1073/pnas.1010082108. Epub 2010 Dec 28.
  PUBMED    21189298
REFERENCE   2  (bases 1 to 864)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1010082108"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2011-01-18- 18"; volume: "108"; issue: "3"; pages: "1110-5"
COMMENT     Sequence Label: HL 2627 (=HL 770 + pHL 908)
FEATURES             Location/Qualifiers
     source          1..864
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             128..139
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     protein_bind    281..297
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     terminator      complement(390..417)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(509..595)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     748..765
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
ORIGIN
        1 cggcctcgcc ctcgccctcg atctcgaact cgtggccgtt cacggagccc tccatgtgca
       61 ccttgaagcg catgaactcc ttgatgatgg ccatgttatc ctcctcgccc ttgctcacca
      121 tcgtacgttt ctcctcttta ataagcttgt ggataaattg agaacgaaag atcaaaaaaa
      181 aagccagcac ccggctggct aagtaatact ggaagcaatg tgagcaatgt cgtgctttca
      241 ggttctccgc gagggtcttc ctgatcgccc cgggagtatc ttgttatccg ctcacaatgt
      301 caatgttatc cgctcacatt tatggatcct gagcggatac atatttgaat gtatttagaa
      361 aaataaacaa aagagtttgt agaaacgcaa aaaggccatc cgtcaggatg gccttctgct
      421 taatttgatg cctggcagtt tatggcgggc gtcctgcccg ccaccctccg ggccgttgct
      481 tcgcaacgtt caaatccgct cccggcggat ttgtcctact caggagagcg ttcaccgaca
      541 aacaacagat aaaacgaaag gcccagtctt tcgactgagc ctttcgtttt atttgatgcc
      601 tggcagttcc ctactctcgc atggggagac cccacactac catcggcgct acggcgtttc
      661 acttctgagt tcggcatggg gtcaggtggg accaccgcgc tactgccgcc aggcaaattc
      721 tgttttatca gaccgcttct gcgttctgat ttaatctgta tcaggctgaa aatcttctct
      781 catccgccaa aacagccaag ctagctttta tttgtagagc tcatccatgc catgtgtaat
      841 cccagcagct gttacaaact caag
//
