LOCUS       sequence-353210-       11545 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Lentiviral vector for overexpressing transcription factor ORFs with 
            unique 24-bp barcodes. Barcodes facilitate identification of 
            transcription factors in pooled screens..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11545)
  AUTHORS   Joung J, Ma S, Tay T, Geiger-Schuller KR, Kirchgatterer PC, Verdine 
            VK, Guo B, Arias-Garcia MA, Allen WE, Singh A, Kuksenko O, Abudayyeh
            OO, Gootenberg JS, Fu Z, Macrae RK, Buenrostro JD, Regev A, Zhang F
  TITLE     A transcription factor atlas of directed differentiation.
  JOURNAL   Cell. 2023 Jan 5;186(1):209-229.e26. doi: 
            10.1016/j.cell.2022.11.026.
  PUBMED    36608654
REFERENCE   2  (bases 1 to 11545)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2022.11"; journalName: "Cell"; date: "2023-01-5- 5";
            volume: "186"; issue: "1"; pages: "209-229.e26"
COMMENT     Sequence Label: TFORF2560
FEATURES             Location/Qualifiers
     source          1..11545
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..1179
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          232..1170
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1127..1147
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     misc_feature    4483..5071
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4536..4556)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4954..4965)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5143..5376
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5454..5575
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5491..5510)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5545..5564
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5615..5750
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5770..5789)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(5771..5789)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(5799..5816)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(5799..5815)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5808..5830)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5957..6412
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6044..6063)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6254..6275
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        6438..6542
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6543..6611,6612..7403)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6761..6780)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7574..8162
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8063..8082
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8316..8333
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    8450..8471
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(8486..8491,8492..8509,8510..8516)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8524..8540
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     8529..8551
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     8548..8564
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     8548..8564
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     8583..8603
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        8585..8603
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        8631..8857
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8858..9038
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    9085..9210
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9703..9936
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             10121..10165
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        10396..10606
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        10418..10614
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      10465..10600
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             10646..11248
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGAS"
     primer_bind     complement(10646..10665)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     11142..11162
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    11291..11408
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctccggtg cccgtcagtg ggcagagcgc acatcgccca cagtccccga gaagttgggg
       61 ggaggggtcg gcaattgaac cggtgcctag agaaggtggc gcggggtaaa ctgggaaagt
      121 gatgtcgtgt actggctccg cctttttccc gagggtgggg gagaaccgta tataagtgca
      181 gtagtcgccg tgaacgttct ttttcgcaac gggtttgccg ccagaacaca ggtaagtgcc
      241 gtgtgtggtt cccgcgggcc tggcctcttt acgggttatg gcccttgcgt gccttgaatt
      301 acttccacct ggctgcagta cgtgattctt gatcccgagc ttcgggttgg aagtgggtgg
      361 gagagttcga ggccttgcgc ttaaggagcc ccttcgcctc gtgcttgagt tgaggcctgg
      421 cctgggcgct ggggccgccg cgtgcgaatc tggtggcacc ttcgcgcctg tctcgctgct
      481 ttcgataagt ctctagccat ttaaaatttt tgatgacctg ctgcgacgct ttttttctgg
      541 caagatagtc ttgtaaatgc gggccaagat ctgcacactg gtatttcggt ttttggggcc
      601 gcgggcggcg acggggcccg tgcgtcccag cgcacatgtt cggcgaggcg gggcctgcga
      661 gcgcggccac cgagaatcgg acgggggtag tctcaagctg gccggcctgc tctggtgcct
      721 ggcctcgcgc cgccgtgtat cgccccgccc tgggcggcaa ggctggcccg gtcggcacca
      781 gttgcgtgag cggaaagatg gccgcttccc ggccctgctg cagggagctc aaaatggagg
      841 acgcggcgct cgggagagcg ggcgggtgag tcacccacac aaaggaaaag ggcctttccg
      901 tcctcagccg tcgcttcatg tgactccacg gagtaccggg cgccgtccag gcacctcgat
      961 tagttctcga gcttttggag tacgtcgtct ttaggttggg gggaggggtt ttatgcgatg
     1021 gagtttcccc acactgagtg ggtggagact gaagttaggc cagcttggca cttgatgtaa
     1081 ttctccttgg aatttgccct ttttgagttt ggatcttggt tcattctcaa gcctcagaca
     1141 gtggttcaaa gtttttttct tccatttcag gtgtcgtgag gctagcaccg ccaccatgaa
     1201 catgtctcag gcatcagtgt cattccagga cgtgactgtg gaattcaccc gggaggagtg
     1261 gcagcacctg ggccctgtcg agaggacgct gtacagagat gtgatgctgg agaactacag
     1321 ccacctcatc tcagtgggat attgcattac taaacctaag gtgatctcca agttggagaa
     1381 aggagaagag ccatggtctt tagaagatga attcctgaac cagaggtacc cagggtattt
     1441 taaagttgat cacatcaaag ggatccggga aaaacaagaa aaacctctgt ggcaagaaat
     1501 attcatcagt gatgctgaca aaacattgag taaagaagga cagaaagttt tagaaaaacc
     1561 atttaatctg gaaatagctc cagagctttc agaaaaaata tcctgtaaat gtgactcaca
     1621 cagaatgaat ttgccagttg cttctcaatt aattataagt gaaagaaaat attcaagaaa
     1681 gaagactgaa tacatgaatg tgtgtgagaa actgcagctt gatattaagc atgagaaagc
     1741 tcatgctgaa gagaaatctt atgaacatgg tgaaaatgct aaagctttca gttataagaa
     1801 agatcagcat tggaaatttc aaactttgga ggaatctttt gaatgtgatg gatctggaca
     1861 aggtttatat gataagacaa tttgtattac acctcagagt tttctaacag gagaaaagtc
     1921 ctgtaaggat gatgaattta gaaaaaactt tgataaaatc actttattta accacatgag
     1981 aactgacaca agggggaaat gctctgatct taatgaatat gggacatcct gtgacaaaac
     2041 caccgctgtt gaatacaata aagttcacat ggctatgaca cactatgagt gtaatgaaag
     2101 ggggattaat ttcagtagga agtcacccct cactcaatct cagagaacta ttacaggatg
     2161 gagtgctttt gaaagcaata aatgtgaaga aaattttagc cagagctcag cccatatagt
     2221 acatcagaaa acacaagctg gagataaatt tggtgaacat aatgaatgta cagatgccct
     2281 ctaccagaaa ttagacttta cagcacatca gagaattcac acagaagata aattctacct
     2341 ttctgatgaa catgggaaat gcagaaaatc cttttaccgg aaagcacacc tcattcagca
     2401 tcagaggccc cactcaggag agaaaactta ccaatatgag gaatgtgcaa aatccttttg
     2461 ttcaagttca catcctattc agcatcctgg aacttatgtg ggattcaaac tttatgaatg
     2521 taatgaatgt gggaaagctt tctgtcagaa ttcaaacctc agtaaacatc tgagaattca
     2581 cacaaaagag aaaccttgtg ataacaatgg ctgtgggaga tcttacaagt cacccctcat
     2641 aggacaccag aaaacagatg cagagatgga actctgtggt ggcagtgaat atgggaagac
     2701 atcacatctc aaaggacatc agagaattct catgggggag aaaccctatg aatgtattga
     2761 atgtgggaaa actttctcca agacatcaca tctcagagca catcagagaa ttcacacagg
     2821 tgaaaaaccc tatgaatgtg ttgaatgtga gaaaactttc tctcacaaga cacacctcag
     2881 tgtacatcag agagttcaca caggggagaa accctatgaa tgtaatgact gtgggaaatc
     2941 ttttacctat aactcagccc tgagagcaca tcaaagaatt cacacaggtg agaagcccta
     3001 tgaatgcagt gactgtgaga aaacttttgc ccataattca gccctcagag cacatcatag
     3061 aattcacacg ggggagaaac cttatgaatg taatgaatgt ggaaggtctt ttgcccatat
     3121 ttctgttctc aaggcacatc aaagaattca cacaggggag aaaccctatg aatgtaatga
     3181 atgtgggaga tctttcacct acaattcagc cctgagagca catcagagaa ttcacacagg
     3241 tagaaaaccc tatgaatgta gtgactgtga gaaaactttt gcccataatt cagccctcaa
     3301 aatacatcag agaattcaca cgggggagaa accctatgaa tgtaatgaat gtgagaaaac
     3361 atttgcccat aattcagccc ttagagcaca tcagaatatc cacacagggg agaaactcta
     3421 tgaatgtagt gaatgtggaa aaactttttt ccagaagaca cgcctcagta cacatcggag
     3481 aattcacaca ggggagaaac cctatgaatg tagcaagtgt gggaaaactt tctcccagaa
     3541 atcatacctc agtggacatg agagaattca cacaggggaa aaaccgtatg aatgtaacgt
     3601 atgtgggaaa acttttgtct ataaggcagc cctcatagtg catcaaagaa ttcacacagg
     3661 ggagaaaccc tatgaatgta accaatgtgg gaaaactttc tcccaaagaa cacacctctg
     3721 tgcacatcag agaattcata ctggggaaaa accctatgag tgtaatgaat gtgggaaaac
     3781 gtttgctgat aattcagccc tcagggcaca tcacagaatt cacacagggg agaaacccta
     3841 tgaatgtaat gactgtggga agactttctc caagacatca catctcagag cacatcttag
     3901 aactcgctca ggggagaaac cctatgaatg cagtgaatgt gggaaaacct tctctgagaa
     3961 gtcatatgtt agtgcacatc agagagttca tacgggggag aaaccctacg aatgtaatgt
     4021 atgtgggaag ccatttgccc ataattcaac cctcagagta catcaaagaa ttcacacagg
     4081 ggagaaatcc tatgaatgta atgattgtgg gaaaacgttc tcccagaaat cacaccttag
     4141 tgcacaccag agaattcaca caggggagaa accctatgag tgtaatgaat gcggaaaagc
     4201 ttttgcccaa aattcaactc tcagagtaca ccagagaatt cacacagggg agaaacccta
     4261 tgaatgtgat gaatgtggga aaacttttgt ccgtaaggca gctcttaggg tacatcacac
     4321 cagaatgcat accagagaga aaaccctagc atgtaatgga tttgggaagt cctagtaatg
     4381 aactagtccg taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga
     4441 cgaaaacacc gctccgtggg cagaagaacg cgttaagtcg acaatcaacc tctggattac
     4501 aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac gctatgtgga
     4561 tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt cattttctcc
     4621 tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt tgtcaggcaa
     4681 cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg cattgccacc
     4741 acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac ggcggaactc
     4801 atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac tgacaattcc
     4861 gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt tgccacctgg
     4921 attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc ggaccttcct
     4981 tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg ccctcagacg
     5041 agtcggatct ccctttgggc cgcctccccg cgtcgacttt aagaccaatg acttacaagg
     5101 cagctgtaga tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact
     5161 cccaacgaag acaagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct
     5221 gagcctggga gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc
     5281 cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc
     5341 tcagaccctt ttagtcagtg tggaaaatct ctagcagtac gtatagtagt tcatgtcatc
     5401 ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag aggaacttgt
     5461 ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc acaaataaag
     5521 catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta tcttatcatg
     5581 tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc
     5641 cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc
     5701 ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc tagggacgta
     5761 cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt
     5821 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     5881 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     5941 ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt
     6001 acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt cgctttcttc
     6061 ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct
     6121 ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga ttagggtgat
     6181 ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc
     6241 acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc tatctcggtc
     6301 tattcttttg atttataagg gattttgccg atttcggcct attggttaaa aaatgagctg
     6361 atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat ttaggtggca
     6421 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
     6481 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
     6541 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
     6601 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
     6661 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
     6721 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
     6781 cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct cagaatgact
     6841 tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca gtaagagaat
     6901 tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt ctgacaacga
     6961 tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat gtaactcgcc
     7021 ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt gacaccacga
     7081 tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta cttactctag
     7141 cttcccggca acaattaata gactggatgg aggcggataa agttgcagga ccacttctgc
     7201 gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt gagcgtgggt
     7261 ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc gtagttatct
     7321 acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct gagataggtg
     7381 cctcactgat taagcattgg taactgtcag accaagttta ctcatatata ctttagattg
     7441 atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt gataatctca
     7501 tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc gtagaaaaga
     7561 tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa
     7621 aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact ctttttccga
     7681 aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg tagccgtagt
     7741 taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg ctaatcctgt
     7801 taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac tcaagacgat
     7861 agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca cagcccagct
     7921 tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga gaaagcgcca
     7981 cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc ggaacaggag
     8041 agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct gtcgggtttc
     8101 gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg agcctatgga
     8161 aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct tttgctcaca
     8221 tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc tttgagtgag
     8281 ctgataccgc tcgccgcagc cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg
     8341 aagagcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     8401 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     8461 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     8521 gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgccaagc
     8581 gcgcaattaa ccctcactaa agggaacaaa agctggagct gcaagcttaa tgtagtctta
     8641 tgcaatactc ttgtagtctt gcaacatggt aacgatgagt tagcaacatg ccttacaagg
     8701 agagaaaaag caccgtgcat gccgattggt ggaagtaagg tggtacgatc gtgccttatt
     8761 aggaaggcaa cagacgggtc tgacatggat tggacgaacc actgaattgc cgcattgcag
     8821 agatattgta tttaagtgcc tagctcgata cataaacggg tctctctggt tagaccagat
     8881 ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc aataaagctt
     8941 gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta actagagatc
     9001 cctcagaccc ttttagtcag tgtggaaaat ctctagcagt ggcgcccgaa cagggacttg
     9061 aaagcgaaag ggaaaccaga ggagctctct cgacgcagga ctcggcttgc tgaagcgcgc
     9121 acggcaagag gcgaggggcg gcgactggtg agtacgccaa aaattttgac tagcggaggc
     9181 tagaaggaga gagatgggtg cgagagcgtc agtattaagc gggggagaat tagatcgcga
     9241 tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat ataaattaaa acatatagta
     9301 tgggcaagca gggagctaga acgattcgca gttaatcctg gcctgttaga aacatcagaa
     9361 ggctgtagac aaatactggg acagctacaa ccatcccttc agacaggatc agaagaactt
     9421 agatcattat ataatacagt agcaaccctc tattgtgtgc atcaaaggat agagataaaa
     9481 gacaccaagg aagctttaga caagatagag gaagagcaaa acaaaagtaa gaccaccgca
     9541 cagcaagcgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     9601 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     9661 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     9721 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     9781 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     9841 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     9901 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     9961 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
    10021 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
    10081 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
    10141 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
    10201 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
    10261 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
    10321 cccaaccccg aggggaccca tgcattgcat ctcaattagt cagcaaccag gtgtggaaag
    10381 tccccaggct ccccagcagg cagaagtatg caaagcatgc gtctcaatta gtcagcaacc
    10441 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
    10501 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctcggcctct
    10561 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctt
    10621 tctagaggta ccaccggagc ttaccatgac cgagtacaag cccacggtgc gcctcgccac
    10681 ccgcgacgac gtccccaggg ccgtacgcac cctcgccgcc gcgttcgccg actaccccgc
    10741 cacgcgccac accgtcgatc cggaccgcca catcgagcgg gtcaccgagc tgcaagaact
    10801 cttcctcacg cgcgtcgggc tcgacatcgg caaggtgtgg gtcgcggacg acggcgccgc
    10861 cgtggcggtc tggaccacgc cggagagcgt cgaagcgggg gcggtgttcg ccgagatcgg
    10921 cccgcgcatg gccgagttga gcggttcccg gctggccgcg cagcaacaga tggaaggcct
    10981 cctggcgccg caccggccca aggagcccgc gtggttcctg gccaccgtcg gcgtctcgcc
    11041 cgaccaccag ggcaagggtc tgggcagcgc cgtcgtgctc cccggagtgg aggcggccga
    11101 gcgcgccggg gtgcccgcct tcctggagac ctccgcgccc cgcaacctcc ccttctacga
    11161 gcggctcggc ttcaccgtca ccgccgacgt cgaggtgccc gaaggaccgc gcacctggtg
    11221 catgacccgc aagcccggtg ccagttaacg aattctagat cttgagacaa atggcagtat
    11281 tcatccacaa ttttaaaaga aaagggggga ttggggggta cagtgcaggg gaaagaatag
    11341 tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt acaaaaattc
    11401 aaaattttcg ggtttattac agggacagca gagatccact ttggcgccgg ctcgaggcct
    11461 gcaggtgcaa agatggataa agttttaaac agagaggaat ctttgcagct aatggacctt
    11521 ctaggtcttg aaaggagtgg gaatt
//
